Purification and cryo-EM structure determination of Arabidopsis thaliana GLR3.4

Ionotropic glutamate receptors (iGluRs) are ligand-gated ion channels that play crucial roles in the central nervous system. iGluR homologs, termed glutamate receptor-like channels (GLRs), have been found in plants. Investigating the structural and functional relationship between iGluRs and GLRs was...

Descripción completa

Detalles Bibliográficos
Autores principales: Gangwar, Shanti Pal, Green, Marriah N., Yelshanskaya, Maria V., Sobolevsky, Alexander I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496305/
https://www.ncbi.nlm.nih.gov/pubmed/34647037
http://dx.doi.org/10.1016/j.xpro.2021.100855
Descripción
Sumario:Ionotropic glutamate receptors (iGluRs) are ligand-gated ion channels that play crucial roles in the central nervous system. iGluR homologs, termed glutamate receptor-like channels (GLRs), have been found in plants. Investigating the structural and functional relationship between iGluRs and GLRs was limited by GLR protein expression, purification, and structural characterization. Here, we provide a detailed protocol for Arabidopsis thaliana GLR3.4 (AtGLR3.4) expression in a mammalian cell line and purification for structure determination by cryogenic electron microscopy (cryo-EM). For the complete details on the use and execution of this protocol, please refer to Green et al. (2021).

Ejemplares similares