Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis

Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way for the...

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Autores principales: Fujiwara, Ryoga, Sanuki, Rikako, Ajiro, Hiroharu, Fukui, Toshiaki, Yoshida, Shosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497579/
https://www.ncbi.nlm.nih.gov/pubmed/34620982
http://dx.doi.org/10.1038/s41598-021-99528-x
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author Fujiwara, Ryoga
Sanuki, Rikako
Ajiro, Hiroharu
Fukui, Toshiaki
Yoshida, Shosuke
author_facet Fujiwara, Ryoga
Sanuki, Rikako
Ajiro, Hiroharu
Fukui, Toshiaki
Yoshida, Shosuke
author_sort Fujiwara, Ryoga
collection PubMed
description Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way for the bioremediation and bioconversion of PET waste. We found that this strain harbors a poly(hydroxyalkanoate) (PHA) synthesis gene cluster, which is highly homologous with that of Cupriavidus necator, an efficient PHA producer. Cells grown on PET accumulated intracellular PHA at high levels. Collectively, our findings in this study demonstrate that I. sakaiensis can mediate the direct conversion of non-biodegradable PET into environment-friendly plastic, providing a new approach for PET recycling.
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spelling pubmed-84975792021-10-12 Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis Fujiwara, Ryoga Sanuki, Rikako Ajiro, Hiroharu Fukui, Toshiaki Yoshida, Shosuke Sci Rep Article Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way for the bioremediation and bioconversion of PET waste. We found that this strain harbors a poly(hydroxyalkanoate) (PHA) synthesis gene cluster, which is highly homologous with that of Cupriavidus necator, an efficient PHA producer. Cells grown on PET accumulated intracellular PHA at high levels. Collectively, our findings in this study demonstrate that I. sakaiensis can mediate the direct conversion of non-biodegradable PET into environment-friendly plastic, providing a new approach for PET recycling. Nature Publishing Group UK 2021-10-07 /pmc/articles/PMC8497579/ /pubmed/34620982 http://dx.doi.org/10.1038/s41598-021-99528-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Fujiwara, Ryoga
Sanuki, Rikako
Ajiro, Hiroharu
Fukui, Toshiaki
Yoshida, Shosuke
Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_full Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_fullStr Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_full_unstemmed Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_short Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_sort direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by ideonella sakaiensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497579/
https://www.ncbi.nlm.nih.gov/pubmed/34620982
http://dx.doi.org/10.1038/s41598-021-99528-x
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