Contamination-resistant, rapid emulsion-based isothermal nucleic acid amplification with Mie-scatter inspired light scatter analysis for bacterial identification

An emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to reduce the impact that contamination has on assay performance. Ongoing LAMP reactions within the emulsion droplets cause a decrease in interfacial tension, causing a decrease in droplet size, which results in decrea...

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Detalles Bibliográficos
Autores principales: Day, Alexander S., Ulep, Tiffany-Heather, Budiman, Elizabeth, Dieckhaus, Laurel, Safavinia, Babak, Hertenstein, Tyler, Yoon, Jeong-Yeol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497611/
https://www.ncbi.nlm.nih.gov/pubmed/34620908
http://dx.doi.org/10.1038/s41598-021-99200-4

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