Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats

Epithelial–mesenchymal transition (EMT) plays a crucial role in metastasis of epithelial tumors; however, it is challenging to detect EMT by cytology. In the present study, EMT was visualized by fluorescence-immunocytochemistry (FICC). Air-dried smears from epithelial tumors of dogs (n=22) and cats...

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Autores principales: FURUSAWA, Yu, TAKAHASHI, Masashi, SHIMA-SAWA, Mariko, HATAI, Hitoshi, MIYOSHI, Noriaki, YAMATO, Osamu, YABUKI, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498836/
https://www.ncbi.nlm.nih.gov/pubmed/34234057
http://dx.doi.org/10.1292/jvms.21-0021
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author FURUSAWA, Yu
TAKAHASHI, Masashi
SHIMA-SAWA, Mariko
HATAI, Hitoshi
MIYOSHI, Noriaki
YAMATO, Osamu
YABUKI, Akira
author_facet FURUSAWA, Yu
TAKAHASHI, Masashi
SHIMA-SAWA, Mariko
HATAI, Hitoshi
MIYOSHI, Noriaki
YAMATO, Osamu
YABUKI, Akira
author_sort FURUSAWA, Yu
collection PubMed
description Epithelial–mesenchymal transition (EMT) plays a crucial role in metastasis of epithelial tumors; however, it is challenging to detect EMT by cytology. In the present study, EMT was visualized by fluorescence-immunocytochemistry (FICC). Air-dried smears from epithelial tumors of dogs (n=22) and cats (n=9) were stained using mouse monoclonal anti-E-cadherin and rabbit monoclonal anti‐vimentin antibodies. Enzymatic immunohistochemistry (IHC) revealed that 51.6% (8/22 in dogs, 8/9 in cats) of the cases showed EMT. In dogs, FICC could detect EMT in 62.5% (5/8) of those cases. In cats, FICC could detect EMT in 100% (8/8) of the cases. In conclusion, the present FICC method could successfully detect EMT using conventional air-dried cytology smear slides.
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spelling pubmed-84988362021-10-13 Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats FURUSAWA, Yu TAKAHASHI, Masashi SHIMA-SAWA, Mariko HATAI, Hitoshi MIYOSHI, Noriaki YAMATO, Osamu YABUKI, Akira J Vet Med Sci Internal Medicine Epithelial–mesenchymal transition (EMT) plays a crucial role in metastasis of epithelial tumors; however, it is challenging to detect EMT by cytology. In the present study, EMT was visualized by fluorescence-immunocytochemistry (FICC). Air-dried smears from epithelial tumors of dogs (n=22) and cats (n=9) were stained using mouse monoclonal anti-E-cadherin and rabbit monoclonal anti‐vimentin antibodies. Enzymatic immunohistochemistry (IHC) revealed that 51.6% (8/22 in dogs, 8/9 in cats) of the cases showed EMT. In dogs, FICC could detect EMT in 62.5% (5/8) of those cases. In cats, FICC could detect EMT in 100% (8/8) of the cases. In conclusion, the present FICC method could successfully detect EMT using conventional air-dried cytology smear slides. The Japanese Society of Veterinary Science 2021-07-08 2021-09 /pmc/articles/PMC8498836/ /pubmed/34234057 http://dx.doi.org/10.1292/jvms.21-0021 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Internal Medicine
FURUSAWA, Yu
TAKAHASHI, Masashi
SHIMA-SAWA, Mariko
HATAI, Hitoshi
MIYOSHI, Noriaki
YAMATO, Osamu
YABUKI, Akira
Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats
title Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats
title_full Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats
title_fullStr Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats
title_full_unstemmed Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats
title_short Immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats
title_sort immunocytochemical evaluation of epithelial–mesenchymal transition in epithelial tumors of dogs and cats
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498836/
https://www.ncbi.nlm.nih.gov/pubmed/34234057
http://dx.doi.org/10.1292/jvms.21-0021
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