Cargando…
Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila
Quorum sensing (QS) and biofilm formation inhibition activity of esculetin on Aeromonas hydrophila SHAe 115 were evaluated. Exposure to esculetin at 25, 50, and 100μg/ml significantly inhibited the production of protease and hemolysin, the formation of biofilms and attenuated the swarming motility o...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500062/ https://www.ncbi.nlm.nih.gov/pubmed/34630364 http://dx.doi.org/10.3389/fmicb.2021.737626 |
_version_ | 1784580384450674688 |
---|---|
author | Sun, Bing Luo, Huaizhi Jiang, Huan Wang, Zhennan Jia, Aiqun |
author_facet | Sun, Bing Luo, Huaizhi Jiang, Huan Wang, Zhennan Jia, Aiqun |
author_sort | Sun, Bing |
collection | PubMed |
description | Quorum sensing (QS) and biofilm formation inhibition activity of esculetin on Aeromonas hydrophila SHAe 115 were evaluated. Exposure to esculetin at 25, 50, and 100μg/ml significantly inhibited the production of protease and hemolysin, the formation of biofilms and attenuated the swarming motility of A. hydrophila SHAe 115. Biofilm forming inhibition was also observed through confocal laser scanning microscopy and scanning electron microscope. Quantitative real-time PCR analysis indicated that genes positively related to QS and biofilm formation were downregulated to varying degrees, while gene (litR) negatively related to biofilm formation was significantly upregulated. The phenotypic results were in good agreement with gene expression levels. These results indicated that esculetin would be a potential QS inhibitor for A. hydrophila. |
format | Online Article Text |
id | pubmed-8500062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85000622021-10-09 Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila Sun, Bing Luo, Huaizhi Jiang, Huan Wang, Zhennan Jia, Aiqun Front Microbiol Microbiology Quorum sensing (QS) and biofilm formation inhibition activity of esculetin on Aeromonas hydrophila SHAe 115 were evaluated. Exposure to esculetin at 25, 50, and 100μg/ml significantly inhibited the production of protease and hemolysin, the formation of biofilms and attenuated the swarming motility of A. hydrophila SHAe 115. Biofilm forming inhibition was also observed through confocal laser scanning microscopy and scanning electron microscope. Quantitative real-time PCR analysis indicated that genes positively related to QS and biofilm formation were downregulated to varying degrees, while gene (litR) negatively related to biofilm formation was significantly upregulated. The phenotypic results were in good agreement with gene expression levels. These results indicated that esculetin would be a potential QS inhibitor for A. hydrophila. Frontiers Media S.A. 2021-09-24 /pmc/articles/PMC8500062/ /pubmed/34630364 http://dx.doi.org/10.3389/fmicb.2021.737626 Text en Copyright © 2021 Sun, Luo, Jiang, Wang and Jia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Sun, Bing Luo, Huaizhi Jiang, Huan Wang, Zhennan Jia, Aiqun Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila |
title | Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila |
title_full | Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila |
title_fullStr | Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila |
title_full_unstemmed | Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila |
title_short | Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila |
title_sort | inhibition of quorum sensing and biofilm formation of esculetin on aeromonas hydrophila |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500062/ https://www.ncbi.nlm.nih.gov/pubmed/34630364 http://dx.doi.org/10.3389/fmicb.2021.737626 |
work_keys_str_mv | AT sunbing inhibitionofquorumsensingandbiofilmformationofesculetinonaeromonashydrophila AT luohuaizhi inhibitionofquorumsensingandbiofilmformationofesculetinonaeromonashydrophila AT jianghuan inhibitionofquorumsensingandbiofilmformationofesculetinonaeromonashydrophila AT wangzhennan inhibitionofquorumsensingandbiofilmformationofesculetinonaeromonashydrophila AT jiaaiqun inhibitionofquorumsensingandbiofilmformationofesculetinonaeromonashydrophila |