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Chalcomoracin prevents vitreous‐induced activation of AKT and migration of retinal pigment epithelial cells

Retinal pigment epithelial (RPE) cells are the major cell type in the epi‐ or sub‐retinal membranes in the pathogenesis of proliferative vitreoretinopathy (PVR), which is a blinding fibrotic eye disease and still short of effective medicine. The purpose of this study is to demonstrate whether Chaloc...

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Autores principales: Han, Haote, Yang, Yanhui, Liu, Bing, Tian, Jingkui, Dong, Lijun, Qi, Hui, Zhu, Wei, Wang, Jiantao, Lei, Hetian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500972/
https://www.ncbi.nlm.nih.gov/pubmed/34432370
http://dx.doi.org/10.1111/jcmm.16590
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author Han, Haote
Yang, Yanhui
Liu, Bing
Tian, Jingkui
Dong, Lijun
Qi, Hui
Zhu, Wei
Wang, Jiantao
Lei, Hetian
author_facet Han, Haote
Yang, Yanhui
Liu, Bing
Tian, Jingkui
Dong, Lijun
Qi, Hui
Zhu, Wei
Wang, Jiantao
Lei, Hetian
author_sort Han, Haote
collection PubMed
description Retinal pigment epithelial (RPE) cells are the major cell type in the epi‐ or sub‐retinal membranes in the pathogenesis of proliferative vitreoretinopathy (PVR), which is a blinding fibrotic eye disease and still short of effective medicine. The purpose of this study is to demonstrate whether Chalocomoracin (CMR), a novel purified compound from fungus‐infected mulberry leaves, is able to inhibit vitreous‐induced signalling events and cellular responses intrinsic to PVR. Our studies have revealed that the CMR IC50 for ARPE‐19 cells is 35.5 μmol/L at 72 hours, and that 5 μmol/L CMR inhibits vitreous‐induced Akt activation and p53 suppression; in addition, we have discovered that this chemical effectively blocks vitreous‐stimulated proliferation, migration and contraction of ARPE‐19 cells, suggesting that CMR is a promising PVR prophylactic.
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spelling pubmed-85009722021-10-12 Chalcomoracin prevents vitreous‐induced activation of AKT and migration of retinal pigment epithelial cells Han, Haote Yang, Yanhui Liu, Bing Tian, Jingkui Dong, Lijun Qi, Hui Zhu, Wei Wang, Jiantao Lei, Hetian J Cell Mol Med Original Articles Retinal pigment epithelial (RPE) cells are the major cell type in the epi‐ or sub‐retinal membranes in the pathogenesis of proliferative vitreoretinopathy (PVR), which is a blinding fibrotic eye disease and still short of effective medicine. The purpose of this study is to demonstrate whether Chalocomoracin (CMR), a novel purified compound from fungus‐infected mulberry leaves, is able to inhibit vitreous‐induced signalling events and cellular responses intrinsic to PVR. Our studies have revealed that the CMR IC50 for ARPE‐19 cells is 35.5 μmol/L at 72 hours, and that 5 μmol/L CMR inhibits vitreous‐induced Akt activation and p53 suppression; in addition, we have discovered that this chemical effectively blocks vitreous‐stimulated proliferation, migration and contraction of ARPE‐19 cells, suggesting that CMR is a promising PVR prophylactic. John Wiley and Sons Inc. 2021-08-25 2021-10 /pmc/articles/PMC8500972/ /pubmed/34432370 http://dx.doi.org/10.1111/jcmm.16590 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Han, Haote
Yang, Yanhui
Liu, Bing
Tian, Jingkui
Dong, Lijun
Qi, Hui
Zhu, Wei
Wang, Jiantao
Lei, Hetian
Chalcomoracin prevents vitreous‐induced activation of AKT and migration of retinal pigment epithelial cells
title Chalcomoracin prevents vitreous‐induced activation of AKT and migration of retinal pigment epithelial cells
title_full Chalcomoracin prevents vitreous‐induced activation of AKT and migration of retinal pigment epithelial cells
title_fullStr Chalcomoracin prevents vitreous‐induced activation of AKT and migration of retinal pigment epithelial cells
title_full_unstemmed Chalcomoracin prevents vitreous‐induced activation of AKT and migration of retinal pigment epithelial cells
title_short Chalcomoracin prevents vitreous‐induced activation of AKT and migration of retinal pigment epithelial cells
title_sort chalcomoracin prevents vitreous‐induced activation of akt and migration of retinal pigment epithelial cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500972/
https://www.ncbi.nlm.nih.gov/pubmed/34432370
http://dx.doi.org/10.1111/jcmm.16590
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