Cargando…
High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes
Histones are closely related to the state of chromatin, and epigenetic modification of their tail results in regulation in cells. Therefore, developing various analytical tools to map the changes in position and distribution of histone modifications is helpful in studying underlying mechanisms. Here...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501099/ https://www.ncbi.nlm.nih.gov/pubmed/34625566 http://dx.doi.org/10.1038/s41467-021-26224-9 |
_version_ | 1784580581860835328 |
---|---|
author | An, Hyun Ji Kim, Yun Chang, Soojeong Kim, Hakchun Song, Jihwan Park, Hyunsung Choi, Inhee |
author_facet | An, Hyun Ji Kim, Yun Chang, Soojeong Kim, Hakchun Song, Jihwan Park, Hyunsung Choi, Inhee |
author_sort | An, Hyun Ji |
collection | PubMed |
description | Histones are closely related to the state of chromatin, and epigenetic modification of their tail results in regulation in cells. Therefore, developing various analytical tools to map the changes in position and distribution of histone modifications is helpful in studying underlying mechanisms. Herein, we propose a high-spatial and colourimetric imaging method using plasmonic nanoparticles as probes to visualize heterochromatin histone markers in a single nucleus. We visualized the reorganization between repressive histone markers, H3K9me3 and H3K27me3, caused by oncogene-induced senescence based on the scattering colours and spectral shift of plasmonic nanoprobes to longer wavelengths using their distance-dependent coupling effect. The measured scattering profiles were correlated with the computation results simulating the scattering spectra according to the arrangements and distances among the plasmonic nanoprobes. The plasmonic nanoprobe-based high-spatial hyperspectral imaging provides an advanced way to study the dynamics of histone modifications for predicting the progression of diseases or senescence. |
format | Online Article Text |
id | pubmed-8501099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85010992021-10-22 High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes An, Hyun Ji Kim, Yun Chang, Soojeong Kim, Hakchun Song, Jihwan Park, Hyunsung Choi, Inhee Nat Commun Article Histones are closely related to the state of chromatin, and epigenetic modification of their tail results in regulation in cells. Therefore, developing various analytical tools to map the changes in position and distribution of histone modifications is helpful in studying underlying mechanisms. Herein, we propose a high-spatial and colourimetric imaging method using plasmonic nanoparticles as probes to visualize heterochromatin histone markers in a single nucleus. We visualized the reorganization between repressive histone markers, H3K9me3 and H3K27me3, caused by oncogene-induced senescence based on the scattering colours and spectral shift of plasmonic nanoprobes to longer wavelengths using their distance-dependent coupling effect. The measured scattering profiles were correlated with the computation results simulating the scattering spectra according to the arrangements and distances among the plasmonic nanoprobes. The plasmonic nanoprobe-based high-spatial hyperspectral imaging provides an advanced way to study the dynamics of histone modifications for predicting the progression of diseases or senescence. Nature Publishing Group UK 2021-10-08 /pmc/articles/PMC8501099/ /pubmed/34625566 http://dx.doi.org/10.1038/s41467-021-26224-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article An, Hyun Ji Kim, Yun Chang, Soojeong Kim, Hakchun Song, Jihwan Park, Hyunsung Choi, Inhee High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes |
title | High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes |
title_full | High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes |
title_fullStr | High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes |
title_full_unstemmed | High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes |
title_short | High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes |
title_sort | high-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501099/ https://www.ncbi.nlm.nih.gov/pubmed/34625566 http://dx.doi.org/10.1038/s41467-021-26224-9 |
work_keys_str_mv | AT anhyunji highspatialandcolourimetricimagingofhistonemodificationsinsinglesenescentcellsusingplasmonicnanoprobes AT kimyun highspatialandcolourimetricimagingofhistonemodificationsinsinglesenescentcellsusingplasmonicnanoprobes AT changsoojeong highspatialandcolourimetricimagingofhistonemodificationsinsinglesenescentcellsusingplasmonicnanoprobes AT kimhakchun highspatialandcolourimetricimagingofhistonemodificationsinsinglesenescentcellsusingplasmonicnanoprobes AT songjihwan highspatialandcolourimetricimagingofhistonemodificationsinsinglesenescentcellsusingplasmonicnanoprobes AT parkhyunsung highspatialandcolourimetricimagingofhistonemodificationsinsinglesenescentcellsusingplasmonicnanoprobes AT choiinhee highspatialandcolourimetricimagingofhistonemodificationsinsinglesenescentcellsusingplasmonicnanoprobes |