Cargando…
Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes
Sweet cherry fruit cracking is a complex physiological disorder that causes significant economic losses. Despite many years of research there is a lack of understanding of the mechanisms involved in cracking. Here, skin and flesh tissue from the cracking susceptible 'Early Bigi’ and the crackin...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research Network of Computational and Structural Biotechnology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501671/ https://www.ncbi.nlm.nih.gov/pubmed/34667535 http://dx.doi.org/10.1016/j.csbj.2021.09.030 |
_version_ | 1784580734115119104 |
---|---|
author | Michailidis, Michail Karagiannis, Evangelos Bazakos, Christos Tanou, Georgia Ganopoulos, Ioannis Molassiotis, Athanassios |
author_facet | Michailidis, Michail Karagiannis, Evangelos Bazakos, Christos Tanou, Georgia Ganopoulos, Ioannis Molassiotis, Athanassios |
author_sort | Michailidis, Michail |
collection | PubMed |
description | Sweet cherry fruit cracking is a complex physiological disorder that causes significant economic losses. Despite many years of research there is a lack of understanding of the mechanisms involved in cracking. Here, skin and flesh tissue from the cracking susceptible 'Early Bigi’ and the cracking tolerant ‘Regina’ cultivars were sampled prior and just after water dipping treatment to identify water-affected metabolic networks that putatively involved in fruit cracking. Primary metabolites, most strongly those involved in sugars and amino acid metabolism, such as glucose and asparagine, shifted in 'Early Bigi’ compared with ‘Regina’ tissues following water exposure. Comparisons between cultivars, tissues and dipping points identified significant differentially expressed genes. Particularly, genes related to abscisic acid, ethylene biosynthesis, pectin metabolism, expansins and aquaporins were altered in water-exposed tissues. To further characterize the role of these genes in cracking, their single nucleotide variants of the coding regions was studied in another eight sweet cherry cultivars, which differ in their sensitivity to cracking, revealing a strong link mainly between pectin metabolism-related genes and cracking-phenotypes. Integrated metabolomic and transcriptomic profiling uncovered genotypic- and tissue-specific metabolic pathways, including tricarboxylic acid cycle, cell enlargement, lipid and ethanol biosynthesis, and plant defense that putatively are involved in fruit cracking. Based on these results, a model which describes the skin and flesh metabolic reprogramming during water-induced fruit cracking in the susceptible 'Early Bigi’ cultivar is presented. Τhis study can help to explore novel candidate genes and metabolic pathways for cracking tolerance in sweet cherry. |
format | Online Article Text |
id | pubmed-8501671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Research Network of Computational and Structural Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-85016712021-10-18 Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes Michailidis, Michail Karagiannis, Evangelos Bazakos, Christos Tanou, Georgia Ganopoulos, Ioannis Molassiotis, Athanassios Comput Struct Biotechnol J Research Article Sweet cherry fruit cracking is a complex physiological disorder that causes significant economic losses. Despite many years of research there is a lack of understanding of the mechanisms involved in cracking. Here, skin and flesh tissue from the cracking susceptible 'Early Bigi’ and the cracking tolerant ‘Regina’ cultivars were sampled prior and just after water dipping treatment to identify water-affected metabolic networks that putatively involved in fruit cracking. Primary metabolites, most strongly those involved in sugars and amino acid metabolism, such as glucose and asparagine, shifted in 'Early Bigi’ compared with ‘Regina’ tissues following water exposure. Comparisons between cultivars, tissues and dipping points identified significant differentially expressed genes. Particularly, genes related to abscisic acid, ethylene biosynthesis, pectin metabolism, expansins and aquaporins were altered in water-exposed tissues. To further characterize the role of these genes in cracking, their single nucleotide variants of the coding regions was studied in another eight sweet cherry cultivars, which differ in their sensitivity to cracking, revealing a strong link mainly between pectin metabolism-related genes and cracking-phenotypes. Integrated metabolomic and transcriptomic profiling uncovered genotypic- and tissue-specific metabolic pathways, including tricarboxylic acid cycle, cell enlargement, lipid and ethanol biosynthesis, and plant defense that putatively are involved in fruit cracking. Based on these results, a model which describes the skin and flesh metabolic reprogramming during water-induced fruit cracking in the susceptible 'Early Bigi’ cultivar is presented. Τhis study can help to explore novel candidate genes and metabolic pathways for cracking tolerance in sweet cherry. Research Network of Computational and Structural Biotechnology 2021-09-28 /pmc/articles/PMC8501671/ /pubmed/34667535 http://dx.doi.org/10.1016/j.csbj.2021.09.030 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Michailidis, Michail Karagiannis, Evangelos Bazakos, Christos Tanou, Georgia Ganopoulos, Ioannis Molassiotis, Athanassios Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes |
title | Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes |
title_full | Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes |
title_fullStr | Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes |
title_full_unstemmed | Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes |
title_short | Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes |
title_sort | genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501671/ https://www.ncbi.nlm.nih.gov/pubmed/34667535 http://dx.doi.org/10.1016/j.csbj.2021.09.030 |
work_keys_str_mv | AT michailidismichail genotypeandtissuespecificmetabolicnetworksandhubgenesinvolvedinwaterinduceddistinctsweetcherryfruitcrackingphenotypes AT karagiannisevangelos genotypeandtissuespecificmetabolicnetworksandhubgenesinvolvedinwaterinduceddistinctsweetcherryfruitcrackingphenotypes AT bazakoschristos genotypeandtissuespecificmetabolicnetworksandhubgenesinvolvedinwaterinduceddistinctsweetcherryfruitcrackingphenotypes AT tanougeorgia genotypeandtissuespecificmetabolicnetworksandhubgenesinvolvedinwaterinduceddistinctsweetcherryfruitcrackingphenotypes AT ganopoulosioannis genotypeandtissuespecificmetabolicnetworksandhubgenesinvolvedinwaterinduceddistinctsweetcherryfruitcrackingphenotypes AT molassiotisathanassios genotypeandtissuespecificmetabolicnetworksandhubgenesinvolvedinwaterinduceddistinctsweetcherryfruitcrackingphenotypes |