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High throughput in vitro characterization of pectins for pig(let) nutrition

BACKGROUND: Fiber-rich feed components possess prebiotic potential to enhance pig health and are considered a potential solution to the high prevalence of post-weaning diarrhea in pig production under the phased suspension of antibiotics and zinc oxide use. METHODS: We screened the gut microbiota mo...

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Autores principales: Wiese, Maria, Hui, Yan, Holck, Jesper, Sejberg, Jimmy J. P., Daures, Celia, Maas, Evy, Kot, Witold, Borné, Johanna M., Khakimov, Bekzod, Thymann, Thomas, Nielsen, Dennis Sandris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501679/
https://www.ncbi.nlm.nih.gov/pubmed/34627409
http://dx.doi.org/10.1186/s42523-021-00129-w
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author Wiese, Maria
Hui, Yan
Holck, Jesper
Sejberg, Jimmy J. P.
Daures, Celia
Maas, Evy
Kot, Witold
Borné, Johanna M.
Khakimov, Bekzod
Thymann, Thomas
Nielsen, Dennis Sandris
author_facet Wiese, Maria
Hui, Yan
Holck, Jesper
Sejberg, Jimmy J. P.
Daures, Celia
Maas, Evy
Kot, Witold
Borné, Johanna M.
Khakimov, Bekzod
Thymann, Thomas
Nielsen, Dennis Sandris
author_sort Wiese, Maria
collection PubMed
description BACKGROUND: Fiber-rich feed components possess prebiotic potential to enhance pig health and are considered a potential solution to the high prevalence of post-weaning diarrhea in pig production under the phased suspension of antibiotics and zinc oxide use. METHODS: We screened the gut microbiota modulatory properties of pectin substrates prepared from sugar beet within the freshly weaned piglet gut microbiome using an in vitro colon model, the CoMiniGut. We focused on testing a variety (13) of sugar beet-derived pectin substrates with defined structures, as well as known prebiotics such as inulin, fructooligosaccharide (FOS) and galactooligosaccharide (GOS), to gain insights on the structure–function related properties of specific substrates on the weaner gut microbial composition as well as shortchain fatty acid production (SCFA). RESULTS: Sugar beet-derived pectin and rhamnogalacturonan-I selectively increased the relative abundance of Bacteroidetes, specifically Prevotella copri, Bacteroides ovatus, Bacteroides acidificiens, and an unclassified Bacteroides member. The degree of esterification impacted the relative abundance of these species and the SCFA production during the in vitro fermentations. Modified arabinans derived from sugar beet promoted the growth of Blautia, P. copri, Lachnospiraceae members and Limosilactobacillus mucosae and amongst all oligosaccharides tested yielded the highest amount of total SCFA produced after 24 h of fermentation. Sugar beet-derived substrates yielded higher total SCFA concentrations (especially acetic and propionic acid) relative to the known prebiotics inulin, FOS and GOS. CONCLUSION: Our results indicate that the molecular structures of pectin, that can be prepared form just one plant source (sugar beet) can selectively stimulate different GM members, highlighting the potential of utilizing pectin substrates as targeted GM modulatory ingredients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-021-00129-w.
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spelling pubmed-85016792021-10-20 High throughput in vitro characterization of pectins for pig(let) nutrition Wiese, Maria Hui, Yan Holck, Jesper Sejberg, Jimmy J. P. Daures, Celia Maas, Evy Kot, Witold Borné, Johanna M. Khakimov, Bekzod Thymann, Thomas Nielsen, Dennis Sandris Anim Microbiome Research Article BACKGROUND: Fiber-rich feed components possess prebiotic potential to enhance pig health and are considered a potential solution to the high prevalence of post-weaning diarrhea in pig production under the phased suspension of antibiotics and zinc oxide use. METHODS: We screened the gut microbiota modulatory properties of pectin substrates prepared from sugar beet within the freshly weaned piglet gut microbiome using an in vitro colon model, the CoMiniGut. We focused on testing a variety (13) of sugar beet-derived pectin substrates with defined structures, as well as known prebiotics such as inulin, fructooligosaccharide (FOS) and galactooligosaccharide (GOS), to gain insights on the structure–function related properties of specific substrates on the weaner gut microbial composition as well as shortchain fatty acid production (SCFA). RESULTS: Sugar beet-derived pectin and rhamnogalacturonan-I selectively increased the relative abundance of Bacteroidetes, specifically Prevotella copri, Bacteroides ovatus, Bacteroides acidificiens, and an unclassified Bacteroides member. The degree of esterification impacted the relative abundance of these species and the SCFA production during the in vitro fermentations. Modified arabinans derived from sugar beet promoted the growth of Blautia, P. copri, Lachnospiraceae members and Limosilactobacillus mucosae and amongst all oligosaccharides tested yielded the highest amount of total SCFA produced after 24 h of fermentation. Sugar beet-derived substrates yielded higher total SCFA concentrations (especially acetic and propionic acid) relative to the known prebiotics inulin, FOS and GOS. CONCLUSION: Our results indicate that the molecular structures of pectin, that can be prepared form just one plant source (sugar beet) can selectively stimulate different GM members, highlighting the potential of utilizing pectin substrates as targeted GM modulatory ingredients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-021-00129-w. BioMed Central 2021-10-09 /pmc/articles/PMC8501679/ /pubmed/34627409 http://dx.doi.org/10.1186/s42523-021-00129-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Wiese, Maria
Hui, Yan
Holck, Jesper
Sejberg, Jimmy J. P.
Daures, Celia
Maas, Evy
Kot, Witold
Borné, Johanna M.
Khakimov, Bekzod
Thymann, Thomas
Nielsen, Dennis Sandris
High throughput in vitro characterization of pectins for pig(let) nutrition
title High throughput in vitro characterization of pectins for pig(let) nutrition
title_full High throughput in vitro characterization of pectins for pig(let) nutrition
title_fullStr High throughput in vitro characterization of pectins for pig(let) nutrition
title_full_unstemmed High throughput in vitro characterization of pectins for pig(let) nutrition
title_short High throughput in vitro characterization of pectins for pig(let) nutrition
title_sort high throughput in vitro characterization of pectins for pig(let) nutrition
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501679/
https://www.ncbi.nlm.nih.gov/pubmed/34627409
http://dx.doi.org/10.1186/s42523-021-00129-w
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