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Use of pooled serum samples to assess herd disease status using commercially available ELISAs
Pooled samples are used in veterinary and human medicine as a cost-effective approach to monitor disease prevalence. Nonetheless, there is limited information on the effect of pooling on test performance, and research is required to determine the appropriate number of samples which can be pooled. Th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502132/ https://www.ncbi.nlm.nih.gov/pubmed/34626237 http://dx.doi.org/10.1007/s11250-021-02939-1 |
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author | Hernandez-Medrano, Juan Heberth Espinosa-Castillo, Luis Fernando Rodriguez, Ana D. Gutierrez, Carlos G. Wapenaar, Wendela |
author_facet | Hernandez-Medrano, Juan Heberth Espinosa-Castillo, Luis Fernando Rodriguez, Ana D. Gutierrez, Carlos G. Wapenaar, Wendela |
author_sort | Hernandez-Medrano, Juan Heberth |
collection | PubMed |
description | Pooled samples are used in veterinary and human medicine as a cost-effective approach to monitor disease prevalence. Nonetheless, there is limited information on the effect of pooling on test performance, and research is required to determine the appropriate number of samples which can be pooled. Therefore, this study aimed to evaluate the use of pooled serum samples as a herd-level surveillance tool for infectious production-limiting diseases: bovine viral diarrhoea (BVD), infectious bovine rhinotracheitis (IBR), enzootic bovine leukosis (EBL) and Neospora caninum (NC), by investigating the maximum number of samples one can pool to identify one positive animal, using commercial antibody-detection ELISAs. Four positive field standards (PFS), one for each disease, were prepared by pooling highly positive herd-level samples diagnosed using commercially available ELISA tests. These PFS were used to simulate 18 pooled samples ranging from undiluted PFS to a dilution representing 1 positive in 1,000 animals using phosphate-buffered saline as diluent. A 1:10 dilution of the PFS resulted in positive results for IBR, BVD and EBL. Moreover, for IBR and BVD, results were still positive at 1:100 and 1:30 dilutions, respectively. However, for NC, a lower dilution (8:10) was required for a seropositive result. This study indicates that, at herd-level, the use of pooled serum is a useful strategy for monitoring infectious diseases (BVD, IBR and EBL) but not NC, using readily available diagnostic assays. |
format | Online Article Text |
id | pubmed-8502132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-85021322021-10-22 Use of pooled serum samples to assess herd disease status using commercially available ELISAs Hernandez-Medrano, Juan Heberth Espinosa-Castillo, Luis Fernando Rodriguez, Ana D. Gutierrez, Carlos G. Wapenaar, Wendela Trop Anim Health Prod Regular Articles Pooled samples are used in veterinary and human medicine as a cost-effective approach to monitor disease prevalence. Nonetheless, there is limited information on the effect of pooling on test performance, and research is required to determine the appropriate number of samples which can be pooled. Therefore, this study aimed to evaluate the use of pooled serum samples as a herd-level surveillance tool for infectious production-limiting diseases: bovine viral diarrhoea (BVD), infectious bovine rhinotracheitis (IBR), enzootic bovine leukosis (EBL) and Neospora caninum (NC), by investigating the maximum number of samples one can pool to identify one positive animal, using commercial antibody-detection ELISAs. Four positive field standards (PFS), one for each disease, were prepared by pooling highly positive herd-level samples diagnosed using commercially available ELISA tests. These PFS were used to simulate 18 pooled samples ranging from undiluted PFS to a dilution representing 1 positive in 1,000 animals using phosphate-buffered saline as diluent. A 1:10 dilution of the PFS resulted in positive results for IBR, BVD and EBL. Moreover, for IBR and BVD, results were still positive at 1:100 and 1:30 dilutions, respectively. However, for NC, a lower dilution (8:10) was required for a seropositive result. This study indicates that, at herd-level, the use of pooled serum is a useful strategy for monitoring infectious diseases (BVD, IBR and EBL) but not NC, using readily available diagnostic assays. Springer Netherlands 2021-10-09 2021 /pmc/articles/PMC8502132/ /pubmed/34626237 http://dx.doi.org/10.1007/s11250-021-02939-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Regular Articles Hernandez-Medrano, Juan Heberth Espinosa-Castillo, Luis Fernando Rodriguez, Ana D. Gutierrez, Carlos G. Wapenaar, Wendela Use of pooled serum samples to assess herd disease status using commercially available ELISAs |
title | Use of pooled serum samples to assess herd disease status using commercially available ELISAs |
title_full | Use of pooled serum samples to assess herd disease status using commercially available ELISAs |
title_fullStr | Use of pooled serum samples to assess herd disease status using commercially available ELISAs |
title_full_unstemmed | Use of pooled serum samples to assess herd disease status using commercially available ELISAs |
title_short | Use of pooled serum samples to assess herd disease status using commercially available ELISAs |
title_sort | use of pooled serum samples to assess herd disease status using commercially available elisas |
topic | Regular Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502132/ https://www.ncbi.nlm.nih.gov/pubmed/34626237 http://dx.doi.org/10.1007/s11250-021-02939-1 |
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