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Gemella massiliensis sp. nov., a new bacterium isolated from the human sputum

Thanks to its ability to isolate previously uncultured bacterial species, culturomics has dynamized the study of the human microbiota. A new bacterial species, Gemella massiliensis Marseille-P3249(T), was isolated from a sputum sample of a healthy French man. Strain Marseille-P3249(T) is a facultati...

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Detalles Bibliográficos
Autores principales: Mbogning Fonkou, Maxime Descartes, Lo, Cheikh Ibrahima, Mekhalif, Zouina, Bilen, Melhem, Tomei, Enora, Kuete Yimagou, Edmond, Dubourg, Grégory, Raoult, Didier, Fenollar, Florence, Fournier, Pierre-Edouard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502168/
https://www.ncbi.nlm.nih.gov/pubmed/34420080
http://dx.doi.org/10.1007/s00203-021-02493-2
Descripción
Sumario:Thanks to its ability to isolate previously uncultured bacterial species, culturomics has dynamized the study of the human microbiota. A new bacterial species, Gemella massiliensis Marseille-P3249(T), was isolated from a sputum sample of a healthy French man. Strain Marseille-P3249(T) is a facultative anaerobe, catalase-negative, Gram positive, coccus, and unable to sporulate. The major fatty acids were C(16:0) (34%), C(18:1n9) (28%), C(18:0) (15%) and C(18:2n6) (13%). Its 16S rRNA sequence exhibits a 98.3% sequence similarity with Gemella bergeri strain 617-93(T), its phylogenetically closest species with standing in nomenclature. Its digital DNA–DNA hybridization (dDDH) and OrthoANI values with G. bergeri of only 59.7 ± 5.6% and 94.8%, respectively. These values are lower than the thresholds for species delineation (> 70% and > 95%, respectively). This strain grows optimally at 37 °C and its genome is 1.80 Mbp long with a 30.5 mol% G + C content. Based on these results, we propose the creation of the new species Gemella massilienis sp. nov., strain Marseille-P3249(T) (= CSUR P3249 = DSMZ 103940). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00203-021-02493-2.