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SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments

Glioma is the most common primary malignant tumor that occurs in the central nervous system. Gliomas are subdivided according to a combination of microscopic morphological, molecular, and genetic factors. Glioblastoma (GBM) is the most aggressive malignant tumor; however, efficient therapies or spec...

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Autores principales: Yu, Yeuni, Sung, Soon Ki, Lee, Chi Hyung, Ha, Mihyang, Kang, Junho, Kwon, Eun Jung, Kang, Ji Wan, Kim, Youngjoo, Kim, Ga Hyun, Heo, Hye Jin, Lee, Hansong, Kim, Tae Woo, Lee, Yoonsung, Myung, Kyungjae, Oh, Chang-Kyu, Kim, Yun Hak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502821/
https://www.ncbi.nlm.nih.gov/pubmed/34646310
http://dx.doi.org/10.3389/fgene.2021.743786
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author Yu, Yeuni
Sung, Soon Ki
Lee, Chi Hyung
Ha, Mihyang
Kang, Junho
Kwon, Eun Jung
Kang, Ji Wan
Kim, Youngjoo
Kim, Ga Hyun
Heo, Hye Jin
Lee, Hansong
Kim, Tae Woo
Lee, Yoonsung
Myung, Kyungjae
Oh, Chang-Kyu
Kim, Yun Hak
author_facet Yu, Yeuni
Sung, Soon Ki
Lee, Chi Hyung
Ha, Mihyang
Kang, Junho
Kwon, Eun Jung
Kang, Ji Wan
Kim, Youngjoo
Kim, Ga Hyun
Heo, Hye Jin
Lee, Hansong
Kim, Tae Woo
Lee, Yoonsung
Myung, Kyungjae
Oh, Chang-Kyu
Kim, Yun Hak
author_sort Yu, Yeuni
collection PubMed
description Glioma is the most common primary malignant tumor that occurs in the central nervous system. Gliomas are subdivided according to a combination of microscopic morphological, molecular, and genetic factors. Glioblastoma (GBM) is the most aggressive malignant tumor; however, efficient therapies or specific target molecules for GBM have not been developed. We accessed RNA-seq and clinical data from The Cancer Genome Atlas, the Chinese Glioma Genome Atlas, and the GSE16011 dataset, and identified differentially expressed genes (DEGs) that were common to both GBM and lower-grade glioma (LGG) in three independent cohorts. The biological functions of common DEGs were examined using NetworkAnalyst. To evaluate the prognostic performance of common DEGs, we performed Kaplan-Meier and Cox regression analyses. We investigated the function of SOCS3 in the central nervous system using three GBM cell lines as well as zebrafish embryos. There were 168 upregulated genes and 50 downregulated genes that were commom to both GBM and LGG. Through survival analyses, we found that SOCS3 was the only prognostic gene in all cohorts. Inhibition of SOCS3 using siRNA decreased the proliferation of GBM cell lines. We also found that the zebrafish ortholog, socs3b, was associated with brain development through the regulation of cell proliferation in neuronal tissue. While additional mechanistic studies are necessary, our results suggest that SOCS3 is an important biomarker for glioma and that SOCS3 is related to the proliferation of neuronal tissue.
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spelling pubmed-85028212021-10-12 SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments Yu, Yeuni Sung, Soon Ki Lee, Chi Hyung Ha, Mihyang Kang, Junho Kwon, Eun Jung Kang, Ji Wan Kim, Youngjoo Kim, Ga Hyun Heo, Hye Jin Lee, Hansong Kim, Tae Woo Lee, Yoonsung Myung, Kyungjae Oh, Chang-Kyu Kim, Yun Hak Front Genet Genetics Glioma is the most common primary malignant tumor that occurs in the central nervous system. Gliomas are subdivided according to a combination of microscopic morphological, molecular, and genetic factors. Glioblastoma (GBM) is the most aggressive malignant tumor; however, efficient therapies or specific target molecules for GBM have not been developed. We accessed RNA-seq and clinical data from The Cancer Genome Atlas, the Chinese Glioma Genome Atlas, and the GSE16011 dataset, and identified differentially expressed genes (DEGs) that were common to both GBM and lower-grade glioma (LGG) in three independent cohorts. The biological functions of common DEGs were examined using NetworkAnalyst. To evaluate the prognostic performance of common DEGs, we performed Kaplan-Meier and Cox regression analyses. We investigated the function of SOCS3 in the central nervous system using three GBM cell lines as well as zebrafish embryos. There were 168 upregulated genes and 50 downregulated genes that were commom to both GBM and LGG. Through survival analyses, we found that SOCS3 was the only prognostic gene in all cohorts. Inhibition of SOCS3 using siRNA decreased the proliferation of GBM cell lines. We also found that the zebrafish ortholog, socs3b, was associated with brain development through the regulation of cell proliferation in neuronal tissue. While additional mechanistic studies are necessary, our results suggest that SOCS3 is an important biomarker for glioma and that SOCS3 is related to the proliferation of neuronal tissue. Frontiers Media S.A. 2021-09-27 /pmc/articles/PMC8502821/ /pubmed/34646310 http://dx.doi.org/10.3389/fgene.2021.743786 Text en Copyright © 2021 Yu, Sung, Lee, Ha, Kang, Kwon, Kang, Kim, Kim, Heo, Lee, Kim, Lee, Myung, Oh and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Yu, Yeuni
Sung, Soon Ki
Lee, Chi Hyung
Ha, Mihyang
Kang, Junho
Kwon, Eun Jung
Kang, Ji Wan
Kim, Youngjoo
Kim, Ga Hyun
Heo, Hye Jin
Lee, Hansong
Kim, Tae Woo
Lee, Yoonsung
Myung, Kyungjae
Oh, Chang-Kyu
Kim, Yun Hak
SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments
title SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments
title_full SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments
title_fullStr SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments
title_full_unstemmed SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments
title_short SOCS3 is Related to Cell Proliferation in Neuronal Tissue: An Integrated Analysis of Bioinformatics and Experiments
title_sort socs3 is related to cell proliferation in neuronal tissue: an integrated analysis of bioinformatics and experiments
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502821/
https://www.ncbi.nlm.nih.gov/pubmed/34646310
http://dx.doi.org/10.3389/fgene.2021.743786
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