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Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster

Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) is one of a few neurotrophic factors described in Drosophila melanogaster (DmMANF) but its function is still poorly characterized. In the present study we found that DmMANF is expressed in different clusters of clock neurons. In particular,...

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Autores principales: Krzeptowski, Wojciech, Walkowicz, Lucyna, Krzeptowska, Ewelina, Motta, Edyta, Witek, Kacper, Szramel, Joanna, Al Abaquita, Terence, Baster, Zbigniew, Rajfur, Zenon, Rosato, Ezio, Stratoulias, Vassilis, Heino, Tapio I., Pyza, Elżbieta M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502870/
https://www.ncbi.nlm.nih.gov/pubmed/34646147
http://dx.doi.org/10.3389/fphys.2021.705183
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author Krzeptowski, Wojciech
Walkowicz, Lucyna
Krzeptowska, Ewelina
Motta, Edyta
Witek, Kacper
Szramel, Joanna
Al Abaquita, Terence
Baster, Zbigniew
Rajfur, Zenon
Rosato, Ezio
Stratoulias, Vassilis
Heino, Tapio I.
Pyza, Elżbieta M.
author_facet Krzeptowski, Wojciech
Walkowicz, Lucyna
Krzeptowska, Ewelina
Motta, Edyta
Witek, Kacper
Szramel, Joanna
Al Abaquita, Terence
Baster, Zbigniew
Rajfur, Zenon
Rosato, Ezio
Stratoulias, Vassilis
Heino, Tapio I.
Pyza, Elżbieta M.
author_sort Krzeptowski, Wojciech
collection PubMed
description Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) is one of a few neurotrophic factors described in Drosophila melanogaster (DmMANF) but its function is still poorly characterized. In the present study we found that DmMANF is expressed in different clusters of clock neurons. In particular, the PDF-positive large (l-LNv) and small (s-LNv) ventral lateral neurons, the CRYPTOCHROME-positive dorsal lateral neurons (LNd), the group 1 dorsal neurons posterior (DN1p) and different tim-positive cells in the fly’s visual system. Importantly, DmMANF expression in the ventral lateral neurons is not controlled by the clock nor it affects its molecular mechanism. However, silencing DmMANF expression in clock neurons affects the rhythm of locomotor activity in light:dark and constant darkness conditions. Such phenotypes correlate with abnormal morphology of the dorsal projections of the s-LNv and with reduced arborizations of the l-LNv in the medulla of the optic lobe. Additionally, we show that DmMANF is important for normal morphology of the L2 interneurons in the visual system and for the circadian rhythm in the topology of their dendritic tree. Our results indicate that DmMANF is important not only for the development of neurites but also for maintaining circadian plasticity of neurons.
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spelling pubmed-85028702021-10-12 Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster Krzeptowski, Wojciech Walkowicz, Lucyna Krzeptowska, Ewelina Motta, Edyta Witek, Kacper Szramel, Joanna Al Abaquita, Terence Baster, Zbigniew Rajfur, Zenon Rosato, Ezio Stratoulias, Vassilis Heino, Tapio I. Pyza, Elżbieta M. Front Physiol Physiology Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) is one of a few neurotrophic factors described in Drosophila melanogaster (DmMANF) but its function is still poorly characterized. In the present study we found that DmMANF is expressed in different clusters of clock neurons. In particular, the PDF-positive large (l-LNv) and small (s-LNv) ventral lateral neurons, the CRYPTOCHROME-positive dorsal lateral neurons (LNd), the group 1 dorsal neurons posterior (DN1p) and different tim-positive cells in the fly’s visual system. Importantly, DmMANF expression in the ventral lateral neurons is not controlled by the clock nor it affects its molecular mechanism. However, silencing DmMANF expression in clock neurons affects the rhythm of locomotor activity in light:dark and constant darkness conditions. Such phenotypes correlate with abnormal morphology of the dorsal projections of the s-LNv and with reduced arborizations of the l-LNv in the medulla of the optic lobe. Additionally, we show that DmMANF is important for normal morphology of the L2 interneurons in the visual system and for the circadian rhythm in the topology of their dendritic tree. Our results indicate that DmMANF is important not only for the development of neurites but also for maintaining circadian plasticity of neurons. Frontiers Media S.A. 2021-09-27 /pmc/articles/PMC8502870/ /pubmed/34646147 http://dx.doi.org/10.3389/fphys.2021.705183 Text en Copyright © 2021 Krzeptowski, Walkowicz, Krzeptowska, Motta, Witek, Szramel, Al Abaquita, Baster, Rajfur, Rosato, Stratoulias, Heino and Pyza. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Krzeptowski, Wojciech
Walkowicz, Lucyna
Krzeptowska, Ewelina
Motta, Edyta
Witek, Kacper
Szramel, Joanna
Al Abaquita, Terence
Baster, Zbigniew
Rajfur, Zenon
Rosato, Ezio
Stratoulias, Vassilis
Heino, Tapio I.
Pyza, Elżbieta M.
Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster
title Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster
title_full Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster
title_fullStr Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster
title_full_unstemmed Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster
title_short Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in Drosophila melanogaster
title_sort mesencephalic astrocyte-derived neurotrophic factor regulates morphology of pigment-dispersing factor-positive clock neurons and circadian neuronal plasticity in drosophila melanogaster
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502870/
https://www.ncbi.nlm.nih.gov/pubmed/34646147
http://dx.doi.org/10.3389/fphys.2021.705183
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