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Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication

In this study, daidzein microparticles (DMP) were prepared using an improved ultrasound-assisted antisolvent precipitation method. Preliminary experiments were conducted using six single-factor experiments, and principal component analysis (PCA) was adopted to obtain the three staple elements of the...

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Autores principales: Yu, Guoping, Zhu, Hongwei, Huang, Yan, Zhang, Xiaonan, Sun, Lina, Wang, Yutong, Xia, Xinghao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502945/
https://www.ncbi.nlm.nih.gov/pubmed/34624663
http://dx.doi.org/10.1016/j.ultsonch.2021.105772
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author Yu, Guoping
Zhu, Hongwei
Huang, Yan
Zhang, Xiaonan
Sun, Lina
Wang, Yutong
Xia, Xinghao
author_facet Yu, Guoping
Zhu, Hongwei
Huang, Yan
Zhang, Xiaonan
Sun, Lina
Wang, Yutong
Xia, Xinghao
author_sort Yu, Guoping
collection PubMed
description In this study, daidzein microparticles (DMP) were prepared using an improved ultrasound-assisted antisolvent precipitation method. Preliminary experiments were conducted using six single-factor experiments, and principal component analysis (PCA) was adopted to obtain the three staple elements of the ultrasonic power, solution concentration, and nozzle diameter. The response surface Box-Behnken (BBD) design was used to optimize the level of the above factors. The optimal preparation conditions of the DMP were obtained as follows: the flow rate was 4 mL/min, the concentration of the daidzein solution was 16 mg/mL, the ratio of antisolvent to solvent (liquid-to-liquid ratio) was 9, the nozzle diameter was 300 μm, the ultrasonic power was 180 W (665 W/L), and the system speed was 760 r/min. The minimum average particle size of DMP was 181 ± 2 nm. The properties of daidzein particles before and after preparation were analyzed via scanning electron microscopy, X-ray diffraction analysis, Differential scanning calorimetry and Fourier transform infrared spectroscopy, no obvious change in its chemical structure was observed, but crystallinity was reduced. Compared with daidzein powder, DMP has a higher solubility and stronger antioxidant capacity. The above results indicate that the improved method of ultrasonication combined with antisolvent can reduce the size of daidzein particles and has a great potential in practical production.
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spelling pubmed-85029452021-10-15 Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication Yu, Guoping Zhu, Hongwei Huang, Yan Zhang, Xiaonan Sun, Lina Wang, Yutong Xia, Xinghao Ultrason Sonochem Original Research Article In this study, daidzein microparticles (DMP) were prepared using an improved ultrasound-assisted antisolvent precipitation method. Preliminary experiments were conducted using six single-factor experiments, and principal component analysis (PCA) was adopted to obtain the three staple elements of the ultrasonic power, solution concentration, and nozzle diameter. The response surface Box-Behnken (BBD) design was used to optimize the level of the above factors. The optimal preparation conditions of the DMP were obtained as follows: the flow rate was 4 mL/min, the concentration of the daidzein solution was 16 mg/mL, the ratio of antisolvent to solvent (liquid-to-liquid ratio) was 9, the nozzle diameter was 300 μm, the ultrasonic power was 180 W (665 W/L), and the system speed was 760 r/min. The minimum average particle size of DMP was 181 ± 2 nm. The properties of daidzein particles before and after preparation were analyzed via scanning electron microscopy, X-ray diffraction analysis, Differential scanning calorimetry and Fourier transform infrared spectroscopy, no obvious change in its chemical structure was observed, but crystallinity was reduced. Compared with daidzein powder, DMP has a higher solubility and stronger antioxidant capacity. The above results indicate that the improved method of ultrasonication combined with antisolvent can reduce the size of daidzein particles and has a great potential in practical production. Elsevier 2021-10-04 /pmc/articles/PMC8502945/ /pubmed/34624663 http://dx.doi.org/10.1016/j.ultsonch.2021.105772 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
Yu, Guoping
Zhu, Hongwei
Huang, Yan
Zhang, Xiaonan
Sun, Lina
Wang, Yutong
Xia, Xinghao
Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication
title Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication
title_full Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication
title_fullStr Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication
title_full_unstemmed Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication
title_short Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication
title_sort preparation of daidzein microparticles through liquid antisolvent precipitation under ultrasonication
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502945/
https://www.ncbi.nlm.nih.gov/pubmed/34624663
http://dx.doi.org/10.1016/j.ultsonch.2021.105772
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