Generation of recombinant vaccinia virus and analysis of virus-induced cell death

Vaccinia virus is a large double-stranded DNA virus that is widely used to express foreign genes from different origins. We generated recombinant vaccinia virus that expresses a viral inhibitor to examine its effect on virus-induced necroptosis. We provide a detailed protocol to describe the generat...

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Detalles Bibliográficos
Autores principales: Liu, Zhijun, Kang, Kidong, Ka-Ming Chan, Francis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503571/
https://www.ncbi.nlm.nih.gov/pubmed/34661172
http://dx.doi.org/10.1016/j.xpro.2021.100871
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author Liu, Zhijun
Kang, Kidong
Ka-Ming Chan, Francis
author_facet Liu, Zhijun
Kang, Kidong
Ka-Ming Chan, Francis
author_sort Liu, Zhijun
collection PubMed
description Vaccinia virus is a large double-stranded DNA virus that is widely used to express foreign genes from different origins. We generated recombinant vaccinia virus that expresses a viral inhibitor to examine its effect on virus-induced necroptosis. We provide a detailed protocol to describe the generation of recombinant vaccinia virus, validation of protein expression, and determination of necroptosis using live cell imaging. This approach can be adapted to examine the effect of other cell death regulators on virus-induced cell death. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).
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spelling pubmed-85035712021-10-15 Generation of recombinant vaccinia virus and analysis of virus-induced cell death Liu, Zhijun Kang, Kidong Ka-Ming Chan, Francis STAR Protoc Protocol Vaccinia virus is a large double-stranded DNA virus that is widely used to express foreign genes from different origins. We generated recombinant vaccinia virus that expresses a viral inhibitor to examine its effect on virus-induced necroptosis. We provide a detailed protocol to describe the generation of recombinant vaccinia virus, validation of protein expression, and determination of necroptosis using live cell imaging. This approach can be adapted to examine the effect of other cell death regulators on virus-induced cell death. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021). Elsevier 2021-10-04 /pmc/articles/PMC8503571/ /pubmed/34661172 http://dx.doi.org/10.1016/j.xpro.2021.100871 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Zhijun
Kang, Kidong
Ka-Ming Chan, Francis
Generation of recombinant vaccinia virus and analysis of virus-induced cell death
title Generation of recombinant vaccinia virus and analysis of virus-induced cell death
title_full Generation of recombinant vaccinia virus and analysis of virus-induced cell death
title_fullStr Generation of recombinant vaccinia virus and analysis of virus-induced cell death
title_full_unstemmed Generation of recombinant vaccinia virus and analysis of virus-induced cell death
title_short Generation of recombinant vaccinia virus and analysis of virus-induced cell death
title_sort generation of recombinant vaccinia virus and analysis of virus-induced cell death
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503571/
https://www.ncbi.nlm.nih.gov/pubmed/34661172
http://dx.doi.org/10.1016/j.xpro.2021.100871
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