Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy

We describe sample preparation and visualization of fluorescently tagged cellulose synthases in cellulose synthase complexes at the plasma membrane of Arabidopsis hypocotyl epidermal cells using live-cell imaging via spinning disk microscopy. We present a technique for sample mounting that may be su...

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Detalles Bibliográficos
Autores principales: Verbančič, Jana, Huang, Jenny Jiahui, McFarlane, Heather E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503582/
https://www.ncbi.nlm.nih.gov/pubmed/34661171
http://dx.doi.org/10.1016/j.xpro.2021.100863
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author Verbančič, Jana
Huang, Jenny Jiahui
McFarlane, Heather E.
author_facet Verbančič, Jana
Huang, Jenny Jiahui
McFarlane, Heather E.
author_sort Verbančič, Jana
collection PubMed
description We describe sample preparation and visualization of fluorescently tagged cellulose synthases in cellulose synthase complexes at the plasma membrane of Arabidopsis hypocotyl epidermal cells using live-cell imaging via spinning disk microscopy. We present a technique for sample mounting that may be suitable for imaging other samples. Additionally, we offer free, open-source solutions for image analysis and provide extensive troubleshooting suggestions. For complete information on the use and execution of this protocol, please refer to McFarlane et al., 2021.
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spelling pubmed-85035822021-10-15 Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy Verbančič, Jana Huang, Jenny Jiahui McFarlane, Heather E. STAR Protoc Protocol We describe sample preparation and visualization of fluorescently tagged cellulose synthases in cellulose synthase complexes at the plasma membrane of Arabidopsis hypocotyl epidermal cells using live-cell imaging via spinning disk microscopy. We present a technique for sample mounting that may be suitable for imaging other samples. Additionally, we offer free, open-source solutions for image analysis and provide extensive troubleshooting suggestions. For complete information on the use and execution of this protocol, please refer to McFarlane et al., 2021. Elsevier 2021-10-05 /pmc/articles/PMC8503582/ /pubmed/34661171 http://dx.doi.org/10.1016/j.xpro.2021.100863 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Verbančič, Jana
Huang, Jenny Jiahui
McFarlane, Heather E.
Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy
title Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy
title_full Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy
title_fullStr Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy
title_full_unstemmed Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy
title_short Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy
title_sort analysis of cellulose synthase activity in arabidopsis using spinning disk microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503582/
https://www.ncbi.nlm.nih.gov/pubmed/34661171
http://dx.doi.org/10.1016/j.xpro.2021.100863
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