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MicroRNA-100 inhibits breast cancer cell proliferation, invasion and migration by targeting FOXA1

MicroRNAs (miRNAs/miRs) are highly conserved single-stranded small non-coding RNAs, which are involved in the physiological and pathological processes of breast cancer, and affect the prognosis of patients with breast cancer. The present study used the Gene Expression Omnibus (GEO)2R tool to detect...

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Autores principales: Xie, Haihui, Xiao, Ruobing, He, Yaolin, He, Lingzhi, Xie, Changjun, Chen, Juan, Hong, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503813/
https://www.ncbi.nlm.nih.gov/pubmed/34671430
http://dx.doi.org/10.3892/ol.2021.13077
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author Xie, Haihui
Xiao, Ruobing
He, Yaolin
He, Lingzhi
Xie, Changjun
Chen, Juan
Hong, Yan
author_facet Xie, Haihui
Xiao, Ruobing
He, Yaolin
He, Lingzhi
Xie, Changjun
Chen, Juan
Hong, Yan
author_sort Xie, Haihui
collection PubMed
description MicroRNAs (miRNAs/miRs) are highly conserved single-stranded small non-coding RNAs, which are involved in the physiological and pathological processes of breast cancer, and affect the prognosis of patients with breast cancer. The present study used the Gene Expression Omnibus (GEO)2R tool to detect miR-100 expression in breast cancer tissues obtained from GEO breast cancer-related datasets. Bioinformatics analysis revealed that miR-100 expression was downregulated in different stages, grades and lymph node metastasis stages of breast cancer, and patients with high miR-100 expression had a more favorable prognosis. Based on these analyses, Cell Counting Kit-8, wound healing and Transwell assays were performed, and the results demonstrated that overexpression of miR-100 inhibited the proliferation, migration and invasion of breast cancer cells. To verify the tumor-suppressive effect of miR-100 in breast cancer, the LinkedOmics and PITA databases were used to assess the association between miR-100 and forkhead box A1 (FOXA1). The results demonstrated that miR-100 had binding sites within the FOXA1 gene, and FOXA1 expression was negatively associated with miR-100 expression in breast cancer tissues. Similarly, a negative association was observed between miR-100 and FOXA1 expression, using the StarBase V3.0 database. The association between miR-100 and FOXA1 was further verified via reverse transcription-quantitative PCR and western blot analyses, and the dual-luciferase reporter assay. The results demonstrated that miR-100 targeted the 3′-untranslated region of FOXA1 in breast cancer cells. Furthermore, rescue experiments were performed to confirm whether miR-100 exerts its antitumor effects by regulating FOXA1. The results demonstrated that overexpression of FOXA1 promoted the proliferation, migration and invasion of breast cancer cells; thus, the antitumor effects of miR-100 in breast cancer were reversed following overexpression of FOXA1. Taken together, the results of the present study suggest that miR-100 inhibits the proliferation, migration and invasion of breast cancer cells by targeting FOXA1 expression. These results may provide a novel insight and an experimental basis for identifying effective therapeutic targets of high specificity for breast cancer.
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spelling pubmed-85038132021-10-19 MicroRNA-100 inhibits breast cancer cell proliferation, invasion and migration by targeting FOXA1 Xie, Haihui Xiao, Ruobing He, Yaolin He, Lingzhi Xie, Changjun Chen, Juan Hong, Yan Oncol Lett Articles MicroRNAs (miRNAs/miRs) are highly conserved single-stranded small non-coding RNAs, which are involved in the physiological and pathological processes of breast cancer, and affect the prognosis of patients with breast cancer. The present study used the Gene Expression Omnibus (GEO)2R tool to detect miR-100 expression in breast cancer tissues obtained from GEO breast cancer-related datasets. Bioinformatics analysis revealed that miR-100 expression was downregulated in different stages, grades and lymph node metastasis stages of breast cancer, and patients with high miR-100 expression had a more favorable prognosis. Based on these analyses, Cell Counting Kit-8, wound healing and Transwell assays were performed, and the results demonstrated that overexpression of miR-100 inhibited the proliferation, migration and invasion of breast cancer cells. To verify the tumor-suppressive effect of miR-100 in breast cancer, the LinkedOmics and PITA databases were used to assess the association between miR-100 and forkhead box A1 (FOXA1). The results demonstrated that miR-100 had binding sites within the FOXA1 gene, and FOXA1 expression was negatively associated with miR-100 expression in breast cancer tissues. Similarly, a negative association was observed between miR-100 and FOXA1 expression, using the StarBase V3.0 database. The association between miR-100 and FOXA1 was further verified via reverse transcription-quantitative PCR and western blot analyses, and the dual-luciferase reporter assay. The results demonstrated that miR-100 targeted the 3′-untranslated region of FOXA1 in breast cancer cells. Furthermore, rescue experiments were performed to confirm whether miR-100 exerts its antitumor effects by regulating FOXA1. The results demonstrated that overexpression of FOXA1 promoted the proliferation, migration and invasion of breast cancer cells; thus, the antitumor effects of miR-100 in breast cancer were reversed following overexpression of FOXA1. Taken together, the results of the present study suggest that miR-100 inhibits the proliferation, migration and invasion of breast cancer cells by targeting FOXA1 expression. These results may provide a novel insight and an experimental basis for identifying effective therapeutic targets of high specificity for breast cancer. D.A. Spandidos 2021-12 2021-10-01 /pmc/articles/PMC8503813/ /pubmed/34671430 http://dx.doi.org/10.3892/ol.2021.13077 Text en Copyright: © Xie et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xie, Haihui
Xiao, Ruobing
He, Yaolin
He, Lingzhi
Xie, Changjun
Chen, Juan
Hong, Yan
MicroRNA-100 inhibits breast cancer cell proliferation, invasion and migration by targeting FOXA1
title MicroRNA-100 inhibits breast cancer cell proliferation, invasion and migration by targeting FOXA1
title_full MicroRNA-100 inhibits breast cancer cell proliferation, invasion and migration by targeting FOXA1
title_fullStr MicroRNA-100 inhibits breast cancer cell proliferation, invasion and migration by targeting FOXA1
title_full_unstemmed MicroRNA-100 inhibits breast cancer cell proliferation, invasion and migration by targeting FOXA1
title_short MicroRNA-100 inhibits breast cancer cell proliferation, invasion and migration by targeting FOXA1
title_sort microrna-100 inhibits breast cancer cell proliferation, invasion and migration by targeting foxa1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503813/
https://www.ncbi.nlm.nih.gov/pubmed/34671430
http://dx.doi.org/10.3892/ol.2021.13077
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