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Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme
We report on sample IS/17575 since it generated highly divergent results in the Belgian SARS-CoV-2 serology external quality assessment scheme. Sample IS/17575 was serum originating from a 30 years old male patient. 124 diagnostic laboratories analysed this sample. A total of 168 results was returne...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503966/ https://www.ncbi.nlm.nih.gov/pubmed/34773801 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115561 |
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author | Moerman, Alena Vernelen, Kris China, Bernard Capron, Arnaud Bossche, Dorien Van Den Mariën, Joachim Ariën, Kevin K. Van Acker, Jos Delforge, Marie-Luce Reynders, Marijke Boel, An Depypere, Melissa Van Gasse, Natasja Vijgen, Sara Brauner, Jonathan Dujardin, Barbara Padalko, Elizaveta |
author_facet | Moerman, Alena Vernelen, Kris China, Bernard Capron, Arnaud Bossche, Dorien Van Den Mariën, Joachim Ariën, Kevin K. Van Acker, Jos Delforge, Marie-Luce Reynders, Marijke Boel, An Depypere, Melissa Van Gasse, Natasja Vijgen, Sara Brauner, Jonathan Dujardin, Barbara Padalko, Elizaveta |
author_sort | Moerman, Alena |
collection | PubMed |
description | We report on sample IS/17575 since it generated highly divergent results in the Belgian SARS-CoV-2 serology external quality assessment scheme. Sample IS/17575 was serum originating from a 30 years old male patient. 124 diagnostic laboratories analysed this sample. A total of 168 results was returned (including 5 doubles). Overall, 38 were positive. All tests against S1 were positive except the Euroimmun IgG ELISA and the Ortho clinical Diagnostics VITROS IgG CLIA. All tests against S1/S2 (Liaison, Diasorin) resulted in a signal above cutoff. Assays against RBD, mostly generate a negative result. An exception are the Wantai SARS-CoV-2 ELISA's. All tests targeting N protein were negative. The survey shows, when >6 months post-infection, assays targeting at least S1, and preferably S1 combined with S2, are the most sensitive. This finding accentuates the necessity of external quality assessment schedules and importance of antigenic composition of serologic SARS-CoV-2 assays. |
format | Online Article Text |
id | pubmed-8503966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85039662021-10-12 Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme Moerman, Alena Vernelen, Kris China, Bernard Capron, Arnaud Bossche, Dorien Van Den Mariën, Joachim Ariën, Kevin K. Van Acker, Jos Delforge, Marie-Luce Reynders, Marijke Boel, An Depypere, Melissa Van Gasse, Natasja Vijgen, Sara Brauner, Jonathan Dujardin, Barbara Padalko, Elizaveta Diagn Microbiol Infect Dis Virology We report on sample IS/17575 since it generated highly divergent results in the Belgian SARS-CoV-2 serology external quality assessment scheme. Sample IS/17575 was serum originating from a 30 years old male patient. 124 diagnostic laboratories analysed this sample. A total of 168 results was returned (including 5 doubles). Overall, 38 were positive. All tests against S1 were positive except the Euroimmun IgG ELISA and the Ortho clinical Diagnostics VITROS IgG CLIA. All tests against S1/S2 (Liaison, Diasorin) resulted in a signal above cutoff. Assays against RBD, mostly generate a negative result. An exception are the Wantai SARS-CoV-2 ELISA's. All tests targeting N protein were negative. The survey shows, when >6 months post-infection, assays targeting at least S1, and preferably S1 combined with S2, are the most sensitive. This finding accentuates the necessity of external quality assessment schedules and importance of antigenic composition of serologic SARS-CoV-2 assays. Elsevier Inc. 2022-02 2021-10-11 /pmc/articles/PMC8503966/ /pubmed/34773801 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115561 Text en © 2021 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Virology Moerman, Alena Vernelen, Kris China, Bernard Capron, Arnaud Bossche, Dorien Van Den Mariën, Joachim Ariën, Kevin K. Van Acker, Jos Delforge, Marie-Luce Reynders, Marijke Boel, An Depypere, Melissa Van Gasse, Natasja Vijgen, Sara Brauner, Jonathan Dujardin, Barbara Padalko, Elizaveta Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme |
title | Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme |
title_full | Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme |
title_fullStr | Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme |
title_full_unstemmed | Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme |
title_short | Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme |
title_sort | importance of anti-sars-cov-2 assay antigenic composition as revealed by the results of the belgian external quality assessment (eqa) scheme |
topic | Virology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503966/ https://www.ncbi.nlm.nih.gov/pubmed/34773801 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115561 |
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