Cargando…

Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing

Klebsiella pneumoniae (Kp) is the primary causative bacteria for nosocomial infections and hospital outbreaks. In particular, extensively drug-resistant K. pneumoniae (XDRKp) causes severe clinical infections in hospitalized patients. Here, we used pulsed-field gel electrophoresis (PFGE), drug susce...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiong, Liuxin, Su, Lebin, Tan, Hanqing, Zhao, Wansha, Li, Shuying, Zhu, Yingmei, Lu, Limiao, Huang, Zhiwei, Li, Baisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504482/
https://www.ncbi.nlm.nih.gov/pubmed/34646245
http://dx.doi.org/10.3389/fmicb.2021.709356
_version_ 1784581332340310016
author Xiong, Liuxin
Su, Lebin
Tan, Hanqing
Zhao, Wansha
Li, Shuying
Zhu, Yingmei
Lu, Limiao
Huang, Zhiwei
Li, Baisheng
author_facet Xiong, Liuxin
Su, Lebin
Tan, Hanqing
Zhao, Wansha
Li, Shuying
Zhu, Yingmei
Lu, Limiao
Huang, Zhiwei
Li, Baisheng
author_sort Xiong, Liuxin
collection PubMed
description Klebsiella pneumoniae (Kp) is the primary causative bacteria for nosocomial infections and hospital outbreaks. In particular, extensively drug-resistant K. pneumoniae (XDRKp) causes severe clinical infections in hospitalized patients. Here, we used pulsed-field gel electrophoresis (PFGE), drug susceptibility tests, and the whole-genome sequencing (WGS) technology to examine genetic relatedness and phenotypic traits of the strains isolated during an outbreak period. Based on PFGE, a distinct clones cluster comprised of eight XDRKp was observed. These strains were confirmed as ST11-K64 via multiple-locus sequence typing database of Kp. The strains also had genes related to the regulation of biofilm biosynthesis (type 1 & 3 fimbriae, type IV pili biosynthesis, RcsAB, and type VI secretion system) and multiple drug resistance (β-lactamase and aminoglycoside antibiotic resistance). WGS data based on core-single nucleotide polymorphisms and epidemiological investigation showed that the neurosurgery unit was likely the source of the outbreak, the strain was likely to have been transmitted to the ICU through patients. In addition, the two highly probable transmission routes were in the ICU (exposure through shared hospital beds) and the neurosurgery units (all cases were treated by the same rehabilitation physician and were most likely infected during the physical therapy). Notably, the bed mattress had played a crucial transmission role of this outbreak, served as a pathogen reservoir.
format Online
Article
Text
id pubmed-8504482
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85044822021-10-12 Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing Xiong, Liuxin Su, Lebin Tan, Hanqing Zhao, Wansha Li, Shuying Zhu, Yingmei Lu, Limiao Huang, Zhiwei Li, Baisheng Front Microbiol Microbiology Klebsiella pneumoniae (Kp) is the primary causative bacteria for nosocomial infections and hospital outbreaks. In particular, extensively drug-resistant K. pneumoniae (XDRKp) causes severe clinical infections in hospitalized patients. Here, we used pulsed-field gel electrophoresis (PFGE), drug susceptibility tests, and the whole-genome sequencing (WGS) technology to examine genetic relatedness and phenotypic traits of the strains isolated during an outbreak period. Based on PFGE, a distinct clones cluster comprised of eight XDRKp was observed. These strains were confirmed as ST11-K64 via multiple-locus sequence typing database of Kp. The strains also had genes related to the regulation of biofilm biosynthesis (type 1 & 3 fimbriae, type IV pili biosynthesis, RcsAB, and type VI secretion system) and multiple drug resistance (β-lactamase and aminoglycoside antibiotic resistance). WGS data based on core-single nucleotide polymorphisms and epidemiological investigation showed that the neurosurgery unit was likely the source of the outbreak, the strain was likely to have been transmitted to the ICU through patients. In addition, the two highly probable transmission routes were in the ICU (exposure through shared hospital beds) and the neurosurgery units (all cases were treated by the same rehabilitation physician and were most likely infected during the physical therapy). Notably, the bed mattress had played a crucial transmission role of this outbreak, served as a pathogen reservoir. Frontiers Media S.A. 2021-09-27 /pmc/articles/PMC8504482/ /pubmed/34646245 http://dx.doi.org/10.3389/fmicb.2021.709356 Text en Copyright © 2021 Xiong, Su, Tan, Zhao, Li, Zhu, Lu, Huang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xiong, Liuxin
Su, Lebin
Tan, Hanqing
Zhao, Wansha
Li, Shuying
Zhu, Yingmei
Lu, Limiao
Huang, Zhiwei
Li, Baisheng
Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing
title Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing
title_full Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing
title_fullStr Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing
title_full_unstemmed Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing
title_short Molecular Epidemiological Analysis of ST11-K64 Extensively Drug-Resistant Klebsiella pneumoniae Infections Outbreak in Intensive Care and Neurosurgery Units Based on Whole-Genome Sequencing
title_sort molecular epidemiological analysis of st11-k64 extensively drug-resistant klebsiella pneumoniae infections outbreak in intensive care and neurosurgery units based on whole-genome sequencing
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504482/
https://www.ncbi.nlm.nih.gov/pubmed/34646245
http://dx.doi.org/10.3389/fmicb.2021.709356
work_keys_str_mv AT xiongliuxin molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing
AT sulebin molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing
AT tanhanqing molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing
AT zhaowansha molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing
AT lishuying molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing
AT zhuyingmei molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing
AT lulimiao molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing
AT huangzhiwei molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing
AT libaisheng molecularepidemiologicalanalysisofst11k64extensivelydrugresistantklebsiellapneumoniaeinfectionsoutbreakinintensivecareandneurosurgeryunitsbasedonwholegenomesequencing