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Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand

Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand–Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% i...

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Autores principales: Saokham, Kingkan, Hemniam, Nuannapa, Roekwan, Sukanya, Hunsawattanakul, Sirikan, Thawinampan, Jutathip, Siriwan, Wanwisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504725/
https://www.ncbi.nlm.nih.gov/pubmed/34634034
http://dx.doi.org/10.1371/journal.pone.0252846
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author Saokham, Kingkan
Hemniam, Nuannapa
Roekwan, Sukanya
Hunsawattanakul, Sirikan
Thawinampan, Jutathip
Siriwan, Wanwisa
author_facet Saokham, Kingkan
Hemniam, Nuannapa
Roekwan, Sukanya
Hunsawattanakul, Sirikan
Thawinampan, Jutathip
Siriwan, Wanwisa
author_sort Saokham, Kingkan
collection PubMed
description Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand–Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2–3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV—which encodes the capsid protein—showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.
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spelling pubmed-85047252021-10-12 Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand Saokham, Kingkan Hemniam, Nuannapa Roekwan, Sukanya Hunsawattanakul, Sirikan Thawinampan, Jutathip Siriwan, Wanwisa PLoS One Research Article Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand–Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2–3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV—which encodes the capsid protein—showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand. Public Library of Science 2021-10-11 /pmc/articles/PMC8504725/ /pubmed/34634034 http://dx.doi.org/10.1371/journal.pone.0252846 Text en © 2021 Saokham et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Saokham, Kingkan
Hemniam, Nuannapa
Roekwan, Sukanya
Hunsawattanakul, Sirikan
Thawinampan, Jutathip
Siriwan, Wanwisa
Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand
title Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand
title_full Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand
title_fullStr Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand
title_full_unstemmed Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand
title_short Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand
title_sort survey and molecular detection of sri lankan cassava mosaic virus in thailand
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504725/
https://www.ncbi.nlm.nih.gov/pubmed/34634034
http://dx.doi.org/10.1371/journal.pone.0252846
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