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Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients

Cavitation is a major pathological feature of pulmonary tuberculosis (TB). The study is aimed at investigating the mechanism of natural killer (NK) cells participating the cavity formation during Mycobacterium tuberculosis (MTB) infection. Human peripheral blood samples were donated by pulmonary TB...

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Autores principales: Li, Shanshan, Wang, Dongpo, Wei, Panjian, Liu, Rongmei, Guo, Jidong, Yang, Bin, Zhang, Hongtao, Lu, Jie, Gao, Mengqiu, Pang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505075/
https://www.ncbi.nlm.nih.gov/pubmed/34646890
http://dx.doi.org/10.1155/2021/7925903
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author Li, Shanshan
Wang, Dongpo
Wei, Panjian
Liu, Rongmei
Guo, Jidong
Yang, Bin
Zhang, Hongtao
Lu, Jie
Gao, Mengqiu
Pang, Yu
author_facet Li, Shanshan
Wang, Dongpo
Wei, Panjian
Liu, Rongmei
Guo, Jidong
Yang, Bin
Zhang, Hongtao
Lu, Jie
Gao, Mengqiu
Pang, Yu
author_sort Li, Shanshan
collection PubMed
description Cavitation is a major pathological feature of pulmonary tuberculosis (TB). The study is aimed at investigating the mechanism of natural killer (NK) cells participating the cavity formation during Mycobacterium tuberculosis (MTB) infection. Human peripheral blood samples were donated by pulmonary TB patients with cavity or not. Real-time quantitative PCR and enzyme-linked immunosorbent assay were performed to analyze the expression of cytokines secreted by NK cells. And the cytotoxicity of NK cells was compared between two groups. Our data showed that NK cells were more abundant in cohorts of cavity. Increased abundance of granzyme A and granzyme B was observed in culture supernatants of NK cells isolated from cavitary TB patients, which also resulted in a higher level of nonviable MTB-infected monocytes. Our data firstly demonstrates that NK cells participate in cavity formation in pulmonary TB patients. The elevated level and increased cytotoxicity of NK cells accelerate the cavitary formulation.
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spelling pubmed-85050752021-10-12 Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients Li, Shanshan Wang, Dongpo Wei, Panjian Liu, Rongmei Guo, Jidong Yang, Bin Zhang, Hongtao Lu, Jie Gao, Mengqiu Pang, Yu J Immunol Res Research Article Cavitation is a major pathological feature of pulmonary tuberculosis (TB). The study is aimed at investigating the mechanism of natural killer (NK) cells participating the cavity formation during Mycobacterium tuberculosis (MTB) infection. Human peripheral blood samples were donated by pulmonary TB patients with cavity or not. Real-time quantitative PCR and enzyme-linked immunosorbent assay were performed to analyze the expression of cytokines secreted by NK cells. And the cytotoxicity of NK cells was compared between two groups. Our data showed that NK cells were more abundant in cohorts of cavity. Increased abundance of granzyme A and granzyme B was observed in culture supernatants of NK cells isolated from cavitary TB patients, which also resulted in a higher level of nonviable MTB-infected monocytes. Our data firstly demonstrates that NK cells participate in cavity formation in pulmonary TB patients. The elevated level and increased cytotoxicity of NK cells accelerate the cavitary formulation. Hindawi 2021-10-04 /pmc/articles/PMC8505075/ /pubmed/34646890 http://dx.doi.org/10.1155/2021/7925903 Text en Copyright © 2021 Shanshan Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Shanshan
Wang, Dongpo
Wei, Panjian
Liu, Rongmei
Guo, Jidong
Yang, Bin
Zhang, Hongtao
Lu, Jie
Gao, Mengqiu
Pang, Yu
Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients
title Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients
title_full Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients
title_fullStr Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients
title_full_unstemmed Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients
title_short Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients
title_sort elevated natural killer cell-mediated cytotoxicity is associated with cavity formation in pulmonary tuberculosis patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505075/
https://www.ncbi.nlm.nih.gov/pubmed/34646890
http://dx.doi.org/10.1155/2021/7925903
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