Simultaneous orientation and 3D localization microscopy with a Vortex point spread function

Estimating the orientation and 3D position of rotationally constrained emitters with localization microscopy typically requires polarization splitting or a large engineered Point Spread Function (PSF). Here we utilize a compact modified PSF for single molecule emitter imaging to estimate simultaneou...

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Detalles Bibliográficos
Autores principales: Hulleman, Christiaan N., Thorsen, Rasmus Ø., Kim, Eugene, Dekker, Cees, Stallinga, Sjoerd, Rieger, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505439/
https://www.ncbi.nlm.nih.gov/pubmed/34635658
http://dx.doi.org/10.1038/s41467-021-26228-5

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