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What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma?

BACKGROUND: In today's practice, gene-based approaches come to the fore in the determination of prognosis and treatment preferences of multiple myeloma (MM). DNA methylation is one of the new approach parameters. DNA methylation occurs by the addition of a methyl group to cytosines in CpG dinuc...

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Autores principales: Pehlivan, Sacide, Serin, Istemi, Nursal, Ayse Feyda, Oyaci, Yasemin, Gundes, Ilknur, Pehlivan, Mustafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505470/
https://www.ncbi.nlm.nih.gov/pubmed/34637096
http://dx.doi.org/10.1007/s11033-021-06813-z
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author Pehlivan, Sacide
Serin, Istemi
Nursal, Ayse Feyda
Oyaci, Yasemin
Gundes, Ilknur
Pehlivan, Mustafa
author_facet Pehlivan, Sacide
Serin, Istemi
Nursal, Ayse Feyda
Oyaci, Yasemin
Gundes, Ilknur
Pehlivan, Mustafa
author_sort Pehlivan, Sacide
collection PubMed
description BACKGROUND: In today's practice, gene-based approaches come to the fore in the determination of prognosis and treatment preferences of multiple myeloma (MM). DNA methylation is one of the new approach parameters. DNA methylation occurs by the addition of a methyl group to cytosines in CpG dinucleotides. In this study, besides comparing the global DNA and APC 2 gene promotor hypermethylation between our patients with MM and healthy control group, we aimed to demonstrate the effect of hypermethylation on MM treatment responses and survival. METHODS AND RESULTS: 38 patients diagnosed with MM between January 2016 and January 2020 and 50 healthy controls were included in the study. The initial hypermethylation of the patients and the healthy control group were statistically analyzed. In addition, the increase in hypermethylation in the MM group before and after the first series of treatments were analyzed within themselves. There is a significant difference between the patients with MM diagnosis and the healthy control group in terms of the initial global hypermethylation (P = 0.001). In patients with MM, hypermethylation was significantly higher. Global hypermethylation in the post-treatment measurements was significantly increased in comparison to the pre-treatment state (P = 0.012). In terms of APC 2 promotor gene-specific hypermethylation, no significant differences were detected between pre- and post-treatment values (P = 0.368). CONCLUSIONS: This study represents valuable data with the initial global DNA hypermethylation results in the MM patient group and the increase in hypermethylation post-treatment. it will shed light on future studies.
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spelling pubmed-85054702021-10-12 What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma? Pehlivan, Sacide Serin, Istemi Nursal, Ayse Feyda Oyaci, Yasemin Gundes, Ilknur Pehlivan, Mustafa Mol Biol Rep Original Article BACKGROUND: In today's practice, gene-based approaches come to the fore in the determination of prognosis and treatment preferences of multiple myeloma (MM). DNA methylation is one of the new approach parameters. DNA methylation occurs by the addition of a methyl group to cytosines in CpG dinucleotides. In this study, besides comparing the global DNA and APC 2 gene promotor hypermethylation between our patients with MM and healthy control group, we aimed to demonstrate the effect of hypermethylation on MM treatment responses and survival. METHODS AND RESULTS: 38 patients diagnosed with MM between January 2016 and January 2020 and 50 healthy controls were included in the study. The initial hypermethylation of the patients and the healthy control group were statistically analyzed. In addition, the increase in hypermethylation in the MM group before and after the first series of treatments were analyzed within themselves. There is a significant difference between the patients with MM diagnosis and the healthy control group in terms of the initial global hypermethylation (P = 0.001). In patients with MM, hypermethylation was significantly higher. Global hypermethylation in the post-treatment measurements was significantly increased in comparison to the pre-treatment state (P = 0.012). In terms of APC 2 promotor gene-specific hypermethylation, no significant differences were detected between pre- and post-treatment values (P = 0.368). CONCLUSIONS: This study represents valuable data with the initial global DNA hypermethylation results in the MM patient group and the increase in hypermethylation post-treatment. it will shed light on future studies. Springer Netherlands 2021-10-12 2021 /pmc/articles/PMC8505470/ /pubmed/34637096 http://dx.doi.org/10.1007/s11033-021-06813-z Text en © The Author(s), under exclusive licence to Springer Nature B.V. 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Pehlivan, Sacide
Serin, Istemi
Nursal, Ayse Feyda
Oyaci, Yasemin
Gundes, Ilknur
Pehlivan, Mustafa
What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma?
title What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma?
title_full What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma?
title_fullStr What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma?
title_full_unstemmed What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma?
title_short What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma?
title_sort what are the roles of global dna and apc 2 gene promotor hypermethylation in multiple myeloma?
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505470/
https://www.ncbi.nlm.nih.gov/pubmed/34637096
http://dx.doi.org/10.1007/s11033-021-06813-z
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