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What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma?
BACKGROUND: In today's practice, gene-based approaches come to the fore in the determination of prognosis and treatment preferences of multiple myeloma (MM). DNA methylation is one of the new approach parameters. DNA methylation occurs by the addition of a methyl group to cytosines in CpG dinuc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505470/ https://www.ncbi.nlm.nih.gov/pubmed/34637096 http://dx.doi.org/10.1007/s11033-021-06813-z |
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author | Pehlivan, Sacide Serin, Istemi Nursal, Ayse Feyda Oyaci, Yasemin Gundes, Ilknur Pehlivan, Mustafa |
author_facet | Pehlivan, Sacide Serin, Istemi Nursal, Ayse Feyda Oyaci, Yasemin Gundes, Ilknur Pehlivan, Mustafa |
author_sort | Pehlivan, Sacide |
collection | PubMed |
description | BACKGROUND: In today's practice, gene-based approaches come to the fore in the determination of prognosis and treatment preferences of multiple myeloma (MM). DNA methylation is one of the new approach parameters. DNA methylation occurs by the addition of a methyl group to cytosines in CpG dinucleotides. In this study, besides comparing the global DNA and APC 2 gene promotor hypermethylation between our patients with MM and healthy control group, we aimed to demonstrate the effect of hypermethylation on MM treatment responses and survival. METHODS AND RESULTS: 38 patients diagnosed with MM between January 2016 and January 2020 and 50 healthy controls were included in the study. The initial hypermethylation of the patients and the healthy control group were statistically analyzed. In addition, the increase in hypermethylation in the MM group before and after the first series of treatments were analyzed within themselves. There is a significant difference between the patients with MM diagnosis and the healthy control group in terms of the initial global hypermethylation (P = 0.001). In patients with MM, hypermethylation was significantly higher. Global hypermethylation in the post-treatment measurements was significantly increased in comparison to the pre-treatment state (P = 0.012). In terms of APC 2 promotor gene-specific hypermethylation, no significant differences were detected between pre- and post-treatment values (P = 0.368). CONCLUSIONS: This study represents valuable data with the initial global DNA hypermethylation results in the MM patient group and the increase in hypermethylation post-treatment. it will shed light on future studies. |
format | Online Article Text |
id | pubmed-8505470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-85054702021-10-12 What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma? Pehlivan, Sacide Serin, Istemi Nursal, Ayse Feyda Oyaci, Yasemin Gundes, Ilknur Pehlivan, Mustafa Mol Biol Rep Original Article BACKGROUND: In today's practice, gene-based approaches come to the fore in the determination of prognosis and treatment preferences of multiple myeloma (MM). DNA methylation is one of the new approach parameters. DNA methylation occurs by the addition of a methyl group to cytosines in CpG dinucleotides. In this study, besides comparing the global DNA and APC 2 gene promotor hypermethylation between our patients with MM and healthy control group, we aimed to demonstrate the effect of hypermethylation on MM treatment responses and survival. METHODS AND RESULTS: 38 patients diagnosed with MM between January 2016 and January 2020 and 50 healthy controls were included in the study. The initial hypermethylation of the patients and the healthy control group were statistically analyzed. In addition, the increase in hypermethylation in the MM group before and after the first series of treatments were analyzed within themselves. There is a significant difference between the patients with MM diagnosis and the healthy control group in terms of the initial global hypermethylation (P = 0.001). In patients with MM, hypermethylation was significantly higher. Global hypermethylation in the post-treatment measurements was significantly increased in comparison to the pre-treatment state (P = 0.012). In terms of APC 2 promotor gene-specific hypermethylation, no significant differences were detected between pre- and post-treatment values (P = 0.368). CONCLUSIONS: This study represents valuable data with the initial global DNA hypermethylation results in the MM patient group and the increase in hypermethylation post-treatment. it will shed light on future studies. Springer Netherlands 2021-10-12 2021 /pmc/articles/PMC8505470/ /pubmed/34637096 http://dx.doi.org/10.1007/s11033-021-06813-z Text en © The Author(s), under exclusive licence to Springer Nature B.V. 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Pehlivan, Sacide Serin, Istemi Nursal, Ayse Feyda Oyaci, Yasemin Gundes, Ilknur Pehlivan, Mustafa What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma? |
title | What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma? |
title_full | What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma? |
title_fullStr | What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma? |
title_full_unstemmed | What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma? |
title_short | What are the roles of global DNA and APC 2 gene promotor hypermethylation in multiple myeloma? |
title_sort | what are the roles of global dna and apc 2 gene promotor hypermethylation in multiple myeloma? |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505470/ https://www.ncbi.nlm.nih.gov/pubmed/34637096 http://dx.doi.org/10.1007/s11033-021-06813-z |
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