A novel fluorescent probe for detecting hydrogen sulfide in osteoblasts during lipopolysaccharide-mediated inflammation under periodontitis

Periodontitis, one of the most common chronic inflammatory diseases, affects the quality of life. Osteogenesis plays an important role in the disease. There is a connection between hydrogen sulfide (H(2)S) and periodontitis, but according to the study has been published, the precise role of H(2)S in inflammation remains in doubt. The main reason for the lack of research is that H(2)S is an endogenous gasotransmitter, difficult to discern through testing. So, we synthesized a novel fluorescence probe which can detect H(2)S in vitro. By using the novel H(2)S fluorescence probe, we found that H(2)S changes in osteoblasts mainly by cystathionine-γ-lyase, and H(2)S increases under LPS stimulation. H(2)S could be a potential marker for diagnosis of inflammatory diseases of bone, and might help deepen studies of the changes of H(2)S level and promote the progression on the researches about pathogenesis of periodontitis.