Cargando…

Determination of the Heterogeneity of Intramuscular Fat and Visceral Adipose Tissue From Dezhou Donkey by Lipidomics and Transcriptomics Profiling

Intramuscular fat (IMF) and visceral adipose tissue (VAT) are both lipids, but have significantly different deposition processes. Furthermore, the heterogeneity of lipid molecular characteristics and mechanisms is unclear. Accordingly, this study used non-targeted lipidomics and transcriptomics to a...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Mengmeng, Zhu, Mingxia, Chai, Wenqiong, Wang, Yonghui, Song, Yinghua, Liu, Baoxiu, Cai, Changyun, Song, Yingzi, Sun, Xue, Xue, Peng, Wang, Changfa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505748/
https://www.ncbi.nlm.nih.gov/pubmed/34651009
http://dx.doi.org/10.3389/fnut.2021.746684
_version_ 1784581600832389120
author Li, Mengmeng
Zhu, Mingxia
Chai, Wenqiong
Wang, Yonghui
Song, Yinghua
Liu, Baoxiu
Cai, Changyun
Song, Yingzi
Sun, Xue
Xue, Peng
Wang, Changfa
author_facet Li, Mengmeng
Zhu, Mingxia
Chai, Wenqiong
Wang, Yonghui
Song, Yinghua
Liu, Baoxiu
Cai, Changyun
Song, Yingzi
Sun, Xue
Xue, Peng
Wang, Changfa
author_sort Li, Mengmeng
collection PubMed
description Intramuscular fat (IMF) and visceral adipose tissue (VAT) are both lipids, but have significantly different deposition processes. Furthermore, the heterogeneity of lipid molecular characteristics and mechanisms is unclear. Accordingly, this study used non-targeted lipidomics and transcriptomics to analyze the lipid profiles and metabolism of longissimus dorsi muscle (LDM) and VAT from donkeys. A total of 1,146 and 1,134 lipids belonging to 18 subclasses were identified in LDM and VAT, respectively, with LDM having higher glycerophospholipid (GP) and lower glycerolipid (GL) contents. Polyunsaturated fatty acids (PUFAs) were distributed preferentially at the sn-1 positions in triglycerides (TGs), and sn-2 positions in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The percentage PUFA content in TGs was significantly lower in LDM than in VAT, while the opposite trend was observed for PUFAs in PC and PE. A total of 110 different lipid molecules (72 downregulated and 38 upregulated) were identified in LDM compared with VAT, of which 11 were considered potential lipid markers. These different lipids were involved in 17 metabolic pathways, including GL and GP metabolisms. Of the 578 differentially expressed genes screened, 311 were downregulated and 267 were upregulated in LDM compared with VAT. Enriched ontology analysis of the differentially expressed genes mainly involved sphingolipid signaling pathways, and GP, GL, and sphingolipid metabolisms. Overall, lipidomics and transcriptomics indicated differences in lipid profiles and metabolism in LDM and VAT, providing new perspectives for the study of heterogeneity in IMF and VAT.
format Online
Article
Text
id pubmed-8505748
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85057482021-10-13 Determination of the Heterogeneity of Intramuscular Fat and Visceral Adipose Tissue From Dezhou Donkey by Lipidomics and Transcriptomics Profiling Li, Mengmeng Zhu, Mingxia Chai, Wenqiong Wang, Yonghui Song, Yinghua Liu, Baoxiu Cai, Changyun Song, Yingzi Sun, Xue Xue, Peng Wang, Changfa Front Nutr Nutrition Intramuscular fat (IMF) and visceral adipose tissue (VAT) are both lipids, but have significantly different deposition processes. Furthermore, the heterogeneity of lipid molecular characteristics and mechanisms is unclear. Accordingly, this study used non-targeted lipidomics and transcriptomics to analyze the lipid profiles and metabolism of longissimus dorsi muscle (LDM) and VAT from donkeys. A total of 1,146 and 1,134 lipids belonging to 18 subclasses were identified in LDM and VAT, respectively, with LDM having higher glycerophospholipid (GP) and lower glycerolipid (GL) contents. Polyunsaturated fatty acids (PUFAs) were distributed preferentially at the sn-1 positions in triglycerides (TGs), and sn-2 positions in phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The percentage PUFA content in TGs was significantly lower in LDM than in VAT, while the opposite trend was observed for PUFAs in PC and PE. A total of 110 different lipid molecules (72 downregulated and 38 upregulated) were identified in LDM compared with VAT, of which 11 were considered potential lipid markers. These different lipids were involved in 17 metabolic pathways, including GL and GP metabolisms. Of the 578 differentially expressed genes screened, 311 were downregulated and 267 were upregulated in LDM compared with VAT. Enriched ontology analysis of the differentially expressed genes mainly involved sphingolipid signaling pathways, and GP, GL, and sphingolipid metabolisms. Overall, lipidomics and transcriptomics indicated differences in lipid profiles and metabolism in LDM and VAT, providing new perspectives for the study of heterogeneity in IMF and VAT. Frontiers Media S.A. 2021-09-28 /pmc/articles/PMC8505748/ /pubmed/34651009 http://dx.doi.org/10.3389/fnut.2021.746684 Text en Copyright © 2021 Li, Zhu, Chai, Wang, Song, Liu, Cai, Song, Sun, Xue and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Li, Mengmeng
Zhu, Mingxia
Chai, Wenqiong
Wang, Yonghui
Song, Yinghua
Liu, Baoxiu
Cai, Changyun
Song, Yingzi
Sun, Xue
Xue, Peng
Wang, Changfa
Determination of the Heterogeneity of Intramuscular Fat and Visceral Adipose Tissue From Dezhou Donkey by Lipidomics and Transcriptomics Profiling
title Determination of the Heterogeneity of Intramuscular Fat and Visceral Adipose Tissue From Dezhou Donkey by Lipidomics and Transcriptomics Profiling
title_full Determination of the Heterogeneity of Intramuscular Fat and Visceral Adipose Tissue From Dezhou Donkey by Lipidomics and Transcriptomics Profiling
title_fullStr Determination of the Heterogeneity of Intramuscular Fat and Visceral Adipose Tissue From Dezhou Donkey by Lipidomics and Transcriptomics Profiling
title_full_unstemmed Determination of the Heterogeneity of Intramuscular Fat and Visceral Adipose Tissue From Dezhou Donkey by Lipidomics and Transcriptomics Profiling
title_short Determination of the Heterogeneity of Intramuscular Fat and Visceral Adipose Tissue From Dezhou Donkey by Lipidomics and Transcriptomics Profiling
title_sort determination of the heterogeneity of intramuscular fat and visceral adipose tissue from dezhou donkey by lipidomics and transcriptomics profiling
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505748/
https://www.ncbi.nlm.nih.gov/pubmed/34651009
http://dx.doi.org/10.3389/fnut.2021.746684
work_keys_str_mv AT limengmeng determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT zhumingxia determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT chaiwenqiong determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT wangyonghui determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT songyinghua determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT liubaoxiu determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT caichangyun determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT songyingzi determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT sunxue determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT xuepeng determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling
AT wangchangfa determinationoftheheterogeneityofintramuscularfatandvisceraladiposetissuefromdezhoudonkeybylipidomicsandtranscriptomicsprofiling