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Tumor-Associated Microbiota in Proximal and Distal Colorectal Cancer and Their Relationships With Clinical Outcomes

The proximal and distal subsites of colorectal cancer (CRC) have distinct differences in their embryonic origin, epidemiology, and prognosis. Therefore, they are not considered as the same disease. However, the possible difference in microbial characterization of the two subsites of CRC is still unc...

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Autores principales: Jin, Min, Shang, Fumei, Wu, Jingjing, Fan, Qilin, Chen, Chen, Fan, Jun, Liu, Li, Nie, Xiu, Zhang, Tao, Cai, Kailin, Ogino, Shuji, Liu, Hongli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506159/
https://www.ncbi.nlm.nih.gov/pubmed/34650531
http://dx.doi.org/10.3389/fmicb.2021.727937
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author Jin, Min
Shang, Fumei
Wu, Jingjing
Fan, Qilin
Chen, Chen
Fan, Jun
Liu, Li
Nie, Xiu
Zhang, Tao
Cai, Kailin
Ogino, Shuji
Liu, Hongli
author_facet Jin, Min
Shang, Fumei
Wu, Jingjing
Fan, Qilin
Chen, Chen
Fan, Jun
Liu, Li
Nie, Xiu
Zhang, Tao
Cai, Kailin
Ogino, Shuji
Liu, Hongli
author_sort Jin, Min
collection PubMed
description The proximal and distal subsites of colorectal cancer (CRC) have distinct differences in their embryonic origin, epidemiology, and prognosis. Therefore, they are not considered as the same disease. However, the possible difference in microbial characterization of the two subsites of CRC is still unclear. In this study, we explored tumor microbiota diversity and composition difference in patients with proximal (N = 187) and distal CRCs (N = 142). This was carried out on cancer tissues and adjacent tissues using bacterial 16S rRNA sequencing. The Kaplan–Meier method was used to analyze the correlation between differential flora and overall survival rate of the patients. It was found that there were significant differences in tumor microbial characteristics between the proximal and distal CRC tissues. The microbiota communities were distinctly richer in the proximal colon tumor tissues than in the distal CRC tissues. Microbial diversity and structure were relatively constant in the paracancerous normal tissues of the proximal and distal colorectum. Generally, microbial communities of CRC tumor tissues were composed of Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Alpha diversity in the proximal and distal CRC tumor tissues was closely related to specific microflora. The abundance of Fusobacteria was associated with age of patient, tumor diameter, and tumor microsatellite instability (MSI) status of the patients. Moreover, Fusobacteria enrichment was associated with poor prognosis especially in patients with proximal colon cancers, but not in patients with distal CRC. In conclusion, proximal and distal subsites of the CRC present distinct microbiota diversity and community structures. The differences indicate that there are different risk factors across anatomical subsites of CRC, which may provide a new strategy for precise prevention and treatment of CRC in the future.
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spelling pubmed-85061592021-10-13 Tumor-Associated Microbiota in Proximal and Distal Colorectal Cancer and Their Relationships With Clinical Outcomes Jin, Min Shang, Fumei Wu, Jingjing Fan, Qilin Chen, Chen Fan, Jun Liu, Li Nie, Xiu Zhang, Tao Cai, Kailin Ogino, Shuji Liu, Hongli Front Microbiol Microbiology The proximal and distal subsites of colorectal cancer (CRC) have distinct differences in their embryonic origin, epidemiology, and prognosis. Therefore, they are not considered as the same disease. However, the possible difference in microbial characterization of the two subsites of CRC is still unclear. In this study, we explored tumor microbiota diversity and composition difference in patients with proximal (N = 187) and distal CRCs (N = 142). This was carried out on cancer tissues and adjacent tissues using bacterial 16S rRNA sequencing. The Kaplan–Meier method was used to analyze the correlation between differential flora and overall survival rate of the patients. It was found that there were significant differences in tumor microbial characteristics between the proximal and distal CRC tissues. The microbiota communities were distinctly richer in the proximal colon tumor tissues than in the distal CRC tissues. Microbial diversity and structure were relatively constant in the paracancerous normal tissues of the proximal and distal colorectum. Generally, microbial communities of CRC tumor tissues were composed of Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Alpha diversity in the proximal and distal CRC tumor tissues was closely related to specific microflora. The abundance of Fusobacteria was associated with age of patient, tumor diameter, and tumor microsatellite instability (MSI) status of the patients. Moreover, Fusobacteria enrichment was associated with poor prognosis especially in patients with proximal colon cancers, but not in patients with distal CRC. In conclusion, proximal and distal subsites of the CRC present distinct microbiota diversity and community structures. The differences indicate that there are different risk factors across anatomical subsites of CRC, which may provide a new strategy for precise prevention and treatment of CRC in the future. Frontiers Media S.A. 2021-09-28 /pmc/articles/PMC8506159/ /pubmed/34650531 http://dx.doi.org/10.3389/fmicb.2021.727937 Text en Copyright © 2021 Jin, Shang, Wu, Fan, Chen, Fan, Liu, Nie, Zhang, Cai, Ogino and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Jin, Min
Shang, Fumei
Wu, Jingjing
Fan, Qilin
Chen, Chen
Fan, Jun
Liu, Li
Nie, Xiu
Zhang, Tao
Cai, Kailin
Ogino, Shuji
Liu, Hongli
Tumor-Associated Microbiota in Proximal and Distal Colorectal Cancer and Their Relationships With Clinical Outcomes
title Tumor-Associated Microbiota in Proximal and Distal Colorectal Cancer and Their Relationships With Clinical Outcomes
title_full Tumor-Associated Microbiota in Proximal and Distal Colorectal Cancer and Their Relationships With Clinical Outcomes
title_fullStr Tumor-Associated Microbiota in Proximal and Distal Colorectal Cancer and Their Relationships With Clinical Outcomes
title_full_unstemmed Tumor-Associated Microbiota in Proximal and Distal Colorectal Cancer and Their Relationships With Clinical Outcomes
title_short Tumor-Associated Microbiota in Proximal and Distal Colorectal Cancer and Their Relationships With Clinical Outcomes
title_sort tumor-associated microbiota in proximal and distal colorectal cancer and their relationships with clinical outcomes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506159/
https://www.ncbi.nlm.nih.gov/pubmed/34650531
http://dx.doi.org/10.3389/fmicb.2021.727937
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