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Role of spindle pole body component 25 in neurodegeneration

BACKGROUND: Aberrant growth and polarization of microglia are critical for pathological initiation and progression of neurodegenerative conditions like Alzheimer’s disease (AD). However, the molecular signals that govern the outgrowth of microglia have not yet been fully determined. Spindle pole bod...

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Autores principales: Cui, Feilun, Xu, Zhipeng, Lv, Yumei, Hu, Jianpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506722/
https://www.ncbi.nlm.nih.gov/pubmed/34733984
http://dx.doi.org/10.21037/atm-21-4064
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author Cui, Feilun
Xu, Zhipeng
Lv, Yumei
Hu, Jianpeng
author_facet Cui, Feilun
Xu, Zhipeng
Lv, Yumei
Hu, Jianpeng
author_sort Cui, Feilun
collection PubMed
description BACKGROUND: Aberrant growth and polarization of microglia are critical for pathological initiation and progression of neurodegenerative conditions like Alzheimer’s disease (AD). However, the molecular signals that govern the outgrowth of microglia have not yet been fully determined. Spindle pole body component 25 (SPC25) is an important part for forming NDC80 complex, which plays a key role in the assembly of the microtubule-binding domain of kinetochores. Nevertheless, the role of SPC25 in microglial growth during neurodegeneration has not been described before, and was thus addressed in the current study. METHODS: We generated an adeno-associated virus (AAV) serotype PHP.B carrying short hairpin RNA (shRNA) for SPC25 (shSPC25) under a microglia-specific TMEM119 promoter (AAV-pTMEM-shSPC25). Serotype PHP.B allowed the virus to cross blood-brain barrier, while TMEM119 promoter allowed specific targeting microglia in vitro and in vivo. We intravenously administrated AAV-pTMEM-shSPC25 to AD-prone APP/PS1 male and female mice and determined this effect on microglia proliferation and mouse behavior. RESULTS: Depletion of SPC25 did not alter polarization of microglia cell polarization in vitro. On the other hand, AD-prone APP/PS1 mice that had received AAV-pTMEM-shSPC25 significantly decreased SPC25 levels in microglia and attenuated microglia proliferation, resulting in significant improvement of the performance of the mice in behavior tests. CONCLUSIONS: Specific depletion of SPC25 in microglia may prevent AD development through suppression of microglia outgrowth. SPC25 may be a promising novel target for preventing AD through microglia.
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spelling pubmed-85067222021-11-02 Role of spindle pole body component 25 in neurodegeneration Cui, Feilun Xu, Zhipeng Lv, Yumei Hu, Jianpeng Ann Transl Med Original Article BACKGROUND: Aberrant growth and polarization of microglia are critical for pathological initiation and progression of neurodegenerative conditions like Alzheimer’s disease (AD). However, the molecular signals that govern the outgrowth of microglia have not yet been fully determined. Spindle pole body component 25 (SPC25) is an important part for forming NDC80 complex, which plays a key role in the assembly of the microtubule-binding domain of kinetochores. Nevertheless, the role of SPC25 in microglial growth during neurodegeneration has not been described before, and was thus addressed in the current study. METHODS: We generated an adeno-associated virus (AAV) serotype PHP.B carrying short hairpin RNA (shRNA) for SPC25 (shSPC25) under a microglia-specific TMEM119 promoter (AAV-pTMEM-shSPC25). Serotype PHP.B allowed the virus to cross blood-brain barrier, while TMEM119 promoter allowed specific targeting microglia in vitro and in vivo. We intravenously administrated AAV-pTMEM-shSPC25 to AD-prone APP/PS1 male and female mice and determined this effect on microglia proliferation and mouse behavior. RESULTS: Depletion of SPC25 did not alter polarization of microglia cell polarization in vitro. On the other hand, AD-prone APP/PS1 mice that had received AAV-pTMEM-shSPC25 significantly decreased SPC25 levels in microglia and attenuated microglia proliferation, resulting in significant improvement of the performance of the mice in behavior tests. CONCLUSIONS: Specific depletion of SPC25 in microglia may prevent AD development through suppression of microglia outgrowth. SPC25 may be a promising novel target for preventing AD through microglia. AME Publishing Company 2021-09 /pmc/articles/PMC8506722/ /pubmed/34733984 http://dx.doi.org/10.21037/atm-21-4064 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Cui, Feilun
Xu, Zhipeng
Lv, Yumei
Hu, Jianpeng
Role of spindle pole body component 25 in neurodegeneration
title Role of spindle pole body component 25 in neurodegeneration
title_full Role of spindle pole body component 25 in neurodegeneration
title_fullStr Role of spindle pole body component 25 in neurodegeneration
title_full_unstemmed Role of spindle pole body component 25 in neurodegeneration
title_short Role of spindle pole body component 25 in neurodegeneration
title_sort role of spindle pole body component 25 in neurodegeneration
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506722/
https://www.ncbi.nlm.nih.gov/pubmed/34733984
http://dx.doi.org/10.21037/atm-21-4064
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