Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila

Unpaired1 (Upd1) is a ligand of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in Drosophila. In this study, using the CRISPR/Cas9 technique, we generate a transgenic fly strain in which a hemagglutinin (HA) epitope tag sequence is inserted into the en...

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Autores principales: Takemura, Masahiko, Lu, Yi-Si, Nakato, Eriko, Nakato, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506834/
https://www.ncbi.nlm.nih.gov/pubmed/34651105
http://dx.doi.org/10.17912/micropub.biology.000387
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author Takemura, Masahiko
Lu, Yi-Si
Nakato, Eriko
Nakato, Hiroshi
author_facet Takemura, Masahiko
Lu, Yi-Si
Nakato, Eriko
Nakato, Hiroshi
author_sort Takemura, Masahiko
collection PubMed
description Unpaired1 (Upd1) is a ligand of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in Drosophila. In this study, using the CRISPR/Cas9 technique, we generate a transgenic fly strain in which a hemagglutinin (HA) epitope tag sequence is inserted into the endogenous locus of the upd1 gene. Anti-HA antibody staining confirms that the distribution of the epitope-tagged Upd1::HA in various tissues is consistent with upd1 expression patterns revealed by previous studies. This transgenic fly strain will be useful in studying the expression, localization, and association partners of Upd1, and thus will contribute to understanding how activation of the JAK/STAT pathway is regulated.
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spelling pubmed-85068342021-10-13 Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila Takemura, Masahiko Lu, Yi-Si Nakato, Eriko Nakato, Hiroshi MicroPubl Biol Materials and Reagents Unpaired1 (Upd1) is a ligand of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in Drosophila. In this study, using the CRISPR/Cas9 technique, we generate a transgenic fly strain in which a hemagglutinin (HA) epitope tag sequence is inserted into the endogenous locus of the upd1 gene. Anti-HA antibody staining confirms that the distribution of the epitope-tagged Upd1::HA in various tissues is consistent with upd1 expression patterns revealed by previous studies. This transgenic fly strain will be useful in studying the expression, localization, and association partners of Upd1, and thus will contribute to understanding how activation of the JAK/STAT pathway is regulated. Caltech Library 2021-08-31 /pmc/articles/PMC8506834/ /pubmed/34651105 http://dx.doi.org/10.17912/micropub.biology.000387 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Takemura, Masahiko
Lu, Yi-Si
Nakato, Eriko
Nakato, Hiroshi
Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
title Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
title_full Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
title_fullStr Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
title_full_unstemmed Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
title_short Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
title_sort endogenous epitope tagging of a jak/stat ligand unpaired1 in drosophila
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506834/
https://www.ncbi.nlm.nih.gov/pubmed/34651105
http://dx.doi.org/10.17912/micropub.biology.000387
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AT nakatoeriko endogenousepitopetaggingofajakstatligandunpaired1indrosophila
AT nakatohiroshi endogenousepitopetaggingofajakstatligandunpaired1indrosophila

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