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Codonopsis pilosula Polysaccharides Alleviate Aβ(1-40)-Induced PC12 Cells Energy Dysmetabolism via CD38/NAD+ Signaling Pathway
BACKGROUND: Alzheimer's disease (AD) is the most common type of dementia and has a complex pathogenesis with no effective treatment. Energy metabolism disorders, as an early pathological event of AD,have attracted attention as a promising area of AD research. Codonopsis pilosula Polysaccharides...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Science Publishers
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506921/ https://www.ncbi.nlm.nih.gov/pubmed/34102973 http://dx.doi.org/10.2174/1567205018666210608103831 |
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author | Hu, Yi R. Xing, San L. Chen, Chuan Shen, Ding Z. Chen, Jiu L. |
author_facet | Hu, Yi R. Xing, San L. Chen, Chuan Shen, Ding Z. Chen, Jiu L. |
author_sort | Hu, Yi R. |
collection | PubMed |
description | BACKGROUND: Alzheimer's disease (AD) is the most common type of dementia and has a complex pathogenesis with no effective treatment. Energy metabolism disorders, as an early pathological event of AD,have attracted attention as a promising area of AD research. Codonopsis pilosula Polysaccharides are the main effective components of Codonopsis pilosula, which have been demonstrated to regulate energy metabolism. METHODS: In order to further study the roles and mechanisms of Codonopsis pilosula polysaccharides in AD, this study used an Aβ(1-40)-induced PC12 cells model to study the protective effects of Codonopsis pilosula polysaccharides and their potential mechanisms in improving energy metabolism dysfunction. RESULTS: The results showed that Aβ(1-40) induced a decrease in PC12 cells viability, energy metabolism molecules (ATP, NAD+, and NAD+/NADH) and Mitochondrial Membrane Potential (MMP) and an increase in ROS. Additionally, it was found that Aβ(1-40) increased CD38 expression related to NAD+ homeostasis, whereas Silent Information Regulation 2 homolog1 (SIRT1, SIRT3), Peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) and SIRT3 activity were decreased. Codonopsis pilosula polysaccharides increased NAD+, NAD+/NADH, SIRT3, SIRT1, and PGC-1α related to NAD+, thus partially recovering ATP. CONCLUSION: Our findings reveal that Codonopsis pilosula polysaccharides protected PC12 cells from Aβ(1-40)-induced damage, suggesting that these components of the Codonopsis pilosula herb may represent an early treatment option for AD patients. |
format | Online Article Text |
id | pubmed-8506921 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Bentham Science Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-85069212021-11-02 Codonopsis pilosula Polysaccharides Alleviate Aβ(1-40)-Induced PC12 Cells Energy Dysmetabolism via CD38/NAD+ Signaling Pathway Hu, Yi R. Xing, San L. Chen, Chuan Shen, Ding Z. Chen, Jiu L. Curr Alzheimer Res Article BACKGROUND: Alzheimer's disease (AD) is the most common type of dementia and has a complex pathogenesis with no effective treatment. Energy metabolism disorders, as an early pathological event of AD,have attracted attention as a promising area of AD research. Codonopsis pilosula Polysaccharides are the main effective components of Codonopsis pilosula, which have been demonstrated to regulate energy metabolism. METHODS: In order to further study the roles and mechanisms of Codonopsis pilosula polysaccharides in AD, this study used an Aβ(1-40)-induced PC12 cells model to study the protective effects of Codonopsis pilosula polysaccharides and their potential mechanisms in improving energy metabolism dysfunction. RESULTS: The results showed that Aβ(1-40) induced a decrease in PC12 cells viability, energy metabolism molecules (ATP, NAD+, and NAD+/NADH) and Mitochondrial Membrane Potential (MMP) and an increase in ROS. Additionally, it was found that Aβ(1-40) increased CD38 expression related to NAD+ homeostasis, whereas Silent Information Regulation 2 homolog1 (SIRT1, SIRT3), Peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) and SIRT3 activity were decreased. Codonopsis pilosula polysaccharides increased NAD+, NAD+/NADH, SIRT3, SIRT1, and PGC-1α related to NAD+, thus partially recovering ATP. CONCLUSION: Our findings reveal that Codonopsis pilosula polysaccharides protected PC12 cells from Aβ(1-40)-induced damage, suggesting that these components of the Codonopsis pilosula herb may represent an early treatment option for AD patients. Bentham Science Publishers 2021-08-06 2021-08-06 /pmc/articles/PMC8506921/ /pubmed/34102973 http://dx.doi.org/10.2174/1567205018666210608103831 Text en © 2021 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited. |
spellingShingle | Article Hu, Yi R. Xing, San L. Chen, Chuan Shen, Ding Z. Chen, Jiu L. Codonopsis pilosula Polysaccharides Alleviate Aβ(1-40)-Induced PC12 Cells Energy Dysmetabolism via CD38/NAD+ Signaling Pathway |
title |
Codonopsis pilosula Polysaccharides Alleviate Aβ(1-40)-Induced PC12 Cells Energy Dysmetabolism via CD38/NAD+ Signaling Pathway |
title_full |
Codonopsis pilosula Polysaccharides Alleviate Aβ(1-40)-Induced PC12 Cells Energy Dysmetabolism via CD38/NAD+ Signaling Pathway |
title_fullStr |
Codonopsis pilosula Polysaccharides Alleviate Aβ(1-40)-Induced PC12 Cells Energy Dysmetabolism via CD38/NAD+ Signaling Pathway |
title_full_unstemmed |
Codonopsis pilosula Polysaccharides Alleviate Aβ(1-40)-Induced PC12 Cells Energy Dysmetabolism via CD38/NAD+ Signaling Pathway |
title_short |
Codonopsis pilosula Polysaccharides Alleviate Aβ(1-40)-Induced PC12 Cells Energy Dysmetabolism via CD38/NAD+ Signaling Pathway |
title_sort | codonopsis pilosula polysaccharides alleviate aβ(1-40)-induced pc12 cells energy dysmetabolism via cd38/nad+ signaling pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506921/ https://www.ncbi.nlm.nih.gov/pubmed/34102973 http://dx.doi.org/10.2174/1567205018666210608103831 |
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