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Microfluidics applications for high-throughput single cell sequencing

The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, which is critical for disease diagnosis and treatment. Substantial evidences show that the majority of traditional gene profiling methods mask the difference of individu...

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Autores principales: Zhou, Wen-min, Yan, Yan-yan, Guo, Qiao-ru, Ji, Hong, Wang, Hui, Xu, Tian-tian, Makabel, Bolat, Pilarsky, Christian, He, Gen, Yu, Xi-yong, Zhang, Jian-ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507141/
https://www.ncbi.nlm.nih.gov/pubmed/34635104
http://dx.doi.org/10.1186/s12951-021-01045-6
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author Zhou, Wen-min
Yan, Yan-yan
Guo, Qiao-ru
Ji, Hong
Wang, Hui
Xu, Tian-tian
Makabel, Bolat
Pilarsky, Christian
He, Gen
Yu, Xi-yong
Zhang, Jian-ye
author_facet Zhou, Wen-min
Yan, Yan-yan
Guo, Qiao-ru
Ji, Hong
Wang, Hui
Xu, Tian-tian
Makabel, Bolat
Pilarsky, Christian
He, Gen
Yu, Xi-yong
Zhang, Jian-ye
author_sort Zhou, Wen-min
collection PubMed
description The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, which is critical for disease diagnosis and treatment. Substantial evidences show that the majority of traditional gene profiling methods mask the difference of individual cells. Single cell sequencing can provide data to characterize the inherent heterogeneity of individual cells, and reveal complex and rare cell populations. Different microfluidic technologies have emerged for single cell researches and become the frontiers and hot topics over the past decade. In this review article, we introduce the processes of single cell sequencing, and review the principles of microfluidics for single cell analysis. Also, we discuss the common high-throughput single cell sequencing technologies along with their advantages and disadvantages. Lastly, microfluidics applications in single cell sequencing technology for the diagnosis of cancers and immune system diseases are briefly illustrated. [Image: see text]
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spelling pubmed-85071412021-10-25 Microfluidics applications for high-throughput single cell sequencing Zhou, Wen-min Yan, Yan-yan Guo, Qiao-ru Ji, Hong Wang, Hui Xu, Tian-tian Makabel, Bolat Pilarsky, Christian He, Gen Yu, Xi-yong Zhang, Jian-ye J Nanobiotechnology Review The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, which is critical for disease diagnosis and treatment. Substantial evidences show that the majority of traditional gene profiling methods mask the difference of individual cells. Single cell sequencing can provide data to characterize the inherent heterogeneity of individual cells, and reveal complex and rare cell populations. Different microfluidic technologies have emerged for single cell researches and become the frontiers and hot topics over the past decade. In this review article, we introduce the processes of single cell sequencing, and review the principles of microfluidics for single cell analysis. Also, we discuss the common high-throughput single cell sequencing technologies along with their advantages and disadvantages. Lastly, microfluidics applications in single cell sequencing technology for the diagnosis of cancers and immune system diseases are briefly illustrated. [Image: see text] BioMed Central 2021-10-11 /pmc/articles/PMC8507141/ /pubmed/34635104 http://dx.doi.org/10.1186/s12951-021-01045-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Review
Zhou, Wen-min
Yan, Yan-yan
Guo, Qiao-ru
Ji, Hong
Wang, Hui
Xu, Tian-tian
Makabel, Bolat
Pilarsky, Christian
He, Gen
Yu, Xi-yong
Zhang, Jian-ye
Microfluidics applications for high-throughput single cell sequencing
title Microfluidics applications for high-throughput single cell sequencing
title_full Microfluidics applications for high-throughput single cell sequencing
title_fullStr Microfluidics applications for high-throughput single cell sequencing
title_full_unstemmed Microfluidics applications for high-throughput single cell sequencing
title_short Microfluidics applications for high-throughput single cell sequencing
title_sort microfluidics applications for high-throughput single cell sequencing
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507141/
https://www.ncbi.nlm.nih.gov/pubmed/34635104
http://dx.doi.org/10.1186/s12951-021-01045-6
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