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Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse
BACKGROUND: Exposure to the ionizing radiation (IR) encountered outside the magnetic field of the Earth poses a persistent threat to the reproductive functions of astronauts. The potential effects of space IR on the circadian rhythms of male reproductive functions have not been well characterized so...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507176/ https://www.ncbi.nlm.nih.gov/pubmed/34635049 http://dx.doi.org/10.1186/s12199-021-01021-4 |
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author | Qin, Fenju Liu, Ningang Nie, Jing Shen, Tao Xu, Yingjie Pan, Shuxian Pei, Hailong Zhou, Guangming |
author_facet | Qin, Fenju Liu, Ningang Nie, Jing Shen, Tao Xu, Yingjie Pan, Shuxian Pei, Hailong Zhou, Guangming |
author_sort | Qin, Fenju |
collection | PubMed |
description | BACKGROUND: Exposure to the ionizing radiation (IR) encountered outside the magnetic field of the Earth poses a persistent threat to the reproductive functions of astronauts. The potential effects of space IR on the circadian rhythms of male reproductive functions have not been well characterized so far. METHODS: Here, we investigated the circadian effects of IR exposure (3 Gy X-rays) on reproductive functional markers in mouse testicular tissue and epididymis at regular intervals over a 24-h day. For each animal, epididymis was tested for sperm motility, and the testis tissue was used for daily sperm production (DSP), testosterone levels, and activities of testicular enzymes (glucose-6-phosphate dehydrogenase (G6PDH), sorbitol dehydrogenase (SDH), lactic dehydrogenase (LDH), and acid phosphatase (ACP)), and the clock genes mRNA expression such as Clock, Bmal1, Ror-α, Ror-β, or Ror-γ. RESULTS: Mice exposed to IR exhibited a disruption in circadian rhythms of reproductive markers, as indicated by decreased sperm motility, increased daily sperm production (DSP), and reduced activities of testis enzymes such as G6PDH, SDH, LDH, and ACP. Moreover, IR exposure also decreased mRNA expression of five clock genes (Clock, Bmal1, Ror-α, Ror-β, or Ror-γ) in testis, with alteration in the rhythm parameters. CONCLUSION: These findings suggested potential health effects of IR exposure on reproductive functions of male astronauts, in terms of both the daily overall level as well as the circadian rhythmicity. |
format | Online Article Text |
id | pubmed-8507176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-85071762021-10-20 Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse Qin, Fenju Liu, Ningang Nie, Jing Shen, Tao Xu, Yingjie Pan, Shuxian Pei, Hailong Zhou, Guangming Environ Health Prev Med Research Article BACKGROUND: Exposure to the ionizing radiation (IR) encountered outside the magnetic field of the Earth poses a persistent threat to the reproductive functions of astronauts. The potential effects of space IR on the circadian rhythms of male reproductive functions have not been well characterized so far. METHODS: Here, we investigated the circadian effects of IR exposure (3 Gy X-rays) on reproductive functional markers in mouse testicular tissue and epididymis at regular intervals over a 24-h day. For each animal, epididymis was tested for sperm motility, and the testis tissue was used for daily sperm production (DSP), testosterone levels, and activities of testicular enzymes (glucose-6-phosphate dehydrogenase (G6PDH), sorbitol dehydrogenase (SDH), lactic dehydrogenase (LDH), and acid phosphatase (ACP)), and the clock genes mRNA expression such as Clock, Bmal1, Ror-α, Ror-β, or Ror-γ. RESULTS: Mice exposed to IR exhibited a disruption in circadian rhythms of reproductive markers, as indicated by decreased sperm motility, increased daily sperm production (DSP), and reduced activities of testis enzymes such as G6PDH, SDH, LDH, and ACP. Moreover, IR exposure also decreased mRNA expression of five clock genes (Clock, Bmal1, Ror-α, Ror-β, or Ror-γ) in testis, with alteration in the rhythm parameters. CONCLUSION: These findings suggested potential health effects of IR exposure on reproductive functions of male astronauts, in terms of both the daily overall level as well as the circadian rhythmicity. BioMed Central 2021-10-11 2021 /pmc/articles/PMC8507176/ /pubmed/34635049 http://dx.doi.org/10.1186/s12199-021-01021-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Qin, Fenju Liu, Ningang Nie, Jing Shen, Tao Xu, Yingjie Pan, Shuxian Pei, Hailong Zhou, Guangming Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse |
title | Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse |
title_full | Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse |
title_fullStr | Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse |
title_full_unstemmed | Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse |
title_short | Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse |
title_sort | circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507176/ https://www.ncbi.nlm.nih.gov/pubmed/34635049 http://dx.doi.org/10.1186/s12199-021-01021-4 |
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