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MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens

BACKGROUND: The overuse of insecticides to control insect vectors has promoted extensive insecticide resistance in mosquitoes. In this study, the functions of microRNA (miR)-279-3p and its target CYP325BB1 in the regulation of deltamethrin resistance in Culex pipiens pallens was investigated. METHOD...

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Autores principales: Li, Xixi, Hu, Shengli, Zhang, Hongbo, Yin, Haitao, Wang, Huan, Zhou, Dan, Sun, Yan, Ma, Lei, Shen, Bo, Zhu, Changliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507342/
https://www.ncbi.nlm.nih.gov/pubmed/34641939
http://dx.doi.org/10.1186/s13071-021-05033-5
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author Li, Xixi
Hu, Shengli
Zhang, Hongbo
Yin, Haitao
Wang, Huan
Zhou, Dan
Sun, Yan
Ma, Lei
Shen, Bo
Zhu, Changliang
author_facet Li, Xixi
Hu, Shengli
Zhang, Hongbo
Yin, Haitao
Wang, Huan
Zhou, Dan
Sun, Yan
Ma, Lei
Shen, Bo
Zhu, Changliang
author_sort Li, Xixi
collection PubMed
description BACKGROUND: The overuse of insecticides to control insect vectors has promoted extensive insecticide resistance in mosquitoes. In this study, the functions of microRNA (miR)-279-3p and its target CYP325BB1 in the regulation of deltamethrin resistance in Culex pipiens pallens was investigated. METHODS: Quantitative real-time reverse transcription PCR was used to detect the expression levels of miR-279-3p and CYP325BB1. Then, the dual-luciferase reporter assay system, RNA interference, CDC bottle bioassay and Cell Counting Kit-8 (CCK-8) assay were used to explore the roles of these molecules in deltamethrin resistance both in vivo and in vitro. RESULTS: The expression patterns of miR-279-3p and CYP325BB1 were compared between deltamethrin-sensitive (DS-strain) and deltamethrin-resistant (DR-strain) mosquitoes. Luciferase activity was downregulated by miR-279-3p, the effect of which was ablated by a mutation of the putative binding site for CYP325BB1. In DR-strain mosquitoes, the expression of miR-279-3p was increased by microinjection and oral feeding of miR-279-3p agomir (mimic). CYP325BB1 mRNA levels were downregulated, which resulted in a higher mortality of the mosquitoes in miR-279-3p mimic-treated groups. In the DS-strain mosquitoes, microinjection of a miR-279-3p inhibitor decreased miR-279-3p expression, whereas the expression of CYP325BB1 was increased; the mortality of these mosquitoes decreased significantly. In addition, overexpression of pIB/V5-His-CYP325BB1 changed the sensitivity of C6/36 cells to deltamethrin in vitro. Also in DR-strain mosquitoes, downregulation of CYP325BB1 expression by microinjection of si-CYP325BB1 increased mosquito mortality in vivo. CONCLUSIONS: These findings provide empirical evidence of the involvement of miRNAs in the regulation of insecticide resistance and indicate that miR-279-3p suppresses the expression of CYP325BB1, which in turn decreases deltamethrin resistance, resulting in increased mosquito mortality. Taken together, the results provide important information for use in the development of future mosquito control strategies. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-85073422021-10-20 MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens Li, Xixi Hu, Shengli Zhang, Hongbo Yin, Haitao Wang, Huan Zhou, Dan Sun, Yan Ma, Lei Shen, Bo Zhu, Changliang Parasit Vectors Research BACKGROUND: The overuse of insecticides to control insect vectors has promoted extensive insecticide resistance in mosquitoes. In this study, the functions of microRNA (miR)-279-3p and its target CYP325BB1 in the regulation of deltamethrin resistance in Culex pipiens pallens was investigated. METHODS: Quantitative real-time reverse transcription PCR was used to detect the expression levels of miR-279-3p and CYP325BB1. Then, the dual-luciferase reporter assay system, RNA interference, CDC bottle bioassay and Cell Counting Kit-8 (CCK-8) assay were used to explore the roles of these molecules in deltamethrin resistance both in vivo and in vitro. RESULTS: The expression patterns of miR-279-3p and CYP325BB1 were compared between deltamethrin-sensitive (DS-strain) and deltamethrin-resistant (DR-strain) mosquitoes. Luciferase activity was downregulated by miR-279-3p, the effect of which was ablated by a mutation of the putative binding site for CYP325BB1. In DR-strain mosquitoes, the expression of miR-279-3p was increased by microinjection and oral feeding of miR-279-3p agomir (mimic). CYP325BB1 mRNA levels were downregulated, which resulted in a higher mortality of the mosquitoes in miR-279-3p mimic-treated groups. In the DS-strain mosquitoes, microinjection of a miR-279-3p inhibitor decreased miR-279-3p expression, whereas the expression of CYP325BB1 was increased; the mortality of these mosquitoes decreased significantly. In addition, overexpression of pIB/V5-His-CYP325BB1 changed the sensitivity of C6/36 cells to deltamethrin in vitro. Also in DR-strain mosquitoes, downregulation of CYP325BB1 expression by microinjection of si-CYP325BB1 increased mosquito mortality in vivo. CONCLUSIONS: These findings provide empirical evidence of the involvement of miRNAs in the regulation of insecticide resistance and indicate that miR-279-3p suppresses the expression of CYP325BB1, which in turn decreases deltamethrin resistance, resulting in increased mosquito mortality. Taken together, the results provide important information for use in the development of future mosquito control strategies. GRAPHICAL ABSTRACT: [Image: see text] BioMed Central 2021-10-12 /pmc/articles/PMC8507342/ /pubmed/34641939 http://dx.doi.org/10.1186/s13071-021-05033-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Xixi
Hu, Shengli
Zhang, Hongbo
Yin, Haitao
Wang, Huan
Zhou, Dan
Sun, Yan
Ma, Lei
Shen, Bo
Zhu, Changliang
MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens
title MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens
title_full MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens
title_fullStr MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens
title_full_unstemmed MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens
title_short MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens
title_sort mir-279-3p regulates deltamethrin resistance through cyp325bb1 in culex pipiens pallens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507342/
https://www.ncbi.nlm.nih.gov/pubmed/34641939
http://dx.doi.org/10.1186/s13071-021-05033-5
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