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Gram‐negative microbiota is related to acute exacerbation in children with asthma

BACKGROUND: The upper‐airway microbiota may be associated with the pathogenesis of asthma and useful for predicting acute exacerbation. However, the relationship between the lower‐airway microbiota and acute exacerbation in children with asthma is not well understood. We evaluated the characteristic...

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Autores principales: Kim, Yoon Hee, Jang, Haerin, Kim, Soo Yeon, Jung, Jae Hwa, Kim, Ga Eun, Park, Mi Reu, Hong, Jung Yeon, Kim, Mi Na, Kim, Eun Gyul, Kim, Min Jung, Kim, Kyung Won, Sohn, Myung Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507365/
https://www.ncbi.nlm.nih.gov/pubmed/34667591
http://dx.doi.org/10.1002/clt2.12069
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author Kim, Yoon Hee
Jang, Haerin
Kim, Soo Yeon
Jung, Jae Hwa
Kim, Ga Eun
Park, Mi Reu
Hong, Jung Yeon
Kim, Mi Na
Kim, Eun Gyul
Kim, Min Jung
Kim, Kyung Won
Sohn, Myung Hyun
author_facet Kim, Yoon Hee
Jang, Haerin
Kim, Soo Yeon
Jung, Jae Hwa
Kim, Ga Eun
Park, Mi Reu
Hong, Jung Yeon
Kim, Mi Na
Kim, Eun Gyul
Kim, Min Jung
Kim, Kyung Won
Sohn, Myung Hyun
author_sort Kim, Yoon Hee
collection PubMed
description BACKGROUND: The upper‐airway microbiota may be associated with the pathogenesis of asthma and useful for predicting acute exacerbation. However, the relationship between the lower‐airway microbiota and acute exacerbation in children with asthma is not well understood. We evaluated the characteristics of the airway microbiome using induced sputum from children with asthma exacerbation and compared the microbiota‐related differences of inflammatory cytokines with those in children with asthma. METHODS: We analysed the microbiome using induced sputum during acute exacerbation of asthma in children. We identified microbial candidates that were prominent in children with asthma exacerbation and compared them with those in children with stable asthma using various analytical methods. The microbial candidates were analysed to determine their association with inflammatory cytokines. We also developed a predictive functional profile using PICRUSt. RESULTS: A total of 95 children with allergic sensitisation including 22 with asthma exacerbation, 67 with stable asthma, and 6 controls were evaluated. We selected 26 microbial candidates whose abundances were significantly increased, decreased, or correlated during acute exacerbation in children with asthma. Among the microbial candidates, Campylobacter, Capnocytophaga, Haemophilus, and Porphyromonas were associated with inflammatory cytokines including macrophage inflammatory protein (MIP)‐1β, programmed death‐ligand 1, and granzyme B. Both Campylobacter and MIP‐1β levels were correlated with sputum eosinophils. Increased lipopolysaccharide biosynthesis and decreased glycan degradation were observed in children with asthma exacerbation. CONCLUSION: Gram‐negative microbes in the lower airway were related to acute exacerbation in children with asthma. These microbes and associated cytokines may play a role in exacerbating asthma in children.
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spelling pubmed-85073652021-10-18 Gram‐negative microbiota is related to acute exacerbation in children with asthma Kim, Yoon Hee Jang, Haerin Kim, Soo Yeon Jung, Jae Hwa Kim, Ga Eun Park, Mi Reu Hong, Jung Yeon Kim, Mi Na Kim, Eun Gyul Kim, Min Jung Kim, Kyung Won Sohn, Myung Hyun Clin Transl Allergy Research BACKGROUND: The upper‐airway microbiota may be associated with the pathogenesis of asthma and useful for predicting acute exacerbation. However, the relationship between the lower‐airway microbiota and acute exacerbation in children with asthma is not well understood. We evaluated the characteristics of the airway microbiome using induced sputum from children with asthma exacerbation and compared the microbiota‐related differences of inflammatory cytokines with those in children with asthma. METHODS: We analysed the microbiome using induced sputum during acute exacerbation of asthma in children. We identified microbial candidates that were prominent in children with asthma exacerbation and compared them with those in children with stable asthma using various analytical methods. The microbial candidates were analysed to determine their association with inflammatory cytokines. We also developed a predictive functional profile using PICRUSt. RESULTS: A total of 95 children with allergic sensitisation including 22 with asthma exacerbation, 67 with stable asthma, and 6 controls were evaluated. We selected 26 microbial candidates whose abundances were significantly increased, decreased, or correlated during acute exacerbation in children with asthma. Among the microbial candidates, Campylobacter, Capnocytophaga, Haemophilus, and Porphyromonas were associated with inflammatory cytokines including macrophage inflammatory protein (MIP)‐1β, programmed death‐ligand 1, and granzyme B. Both Campylobacter and MIP‐1β levels were correlated with sputum eosinophils. Increased lipopolysaccharide biosynthesis and decreased glycan degradation were observed in children with asthma exacerbation. CONCLUSION: Gram‐negative microbes in the lower airway were related to acute exacerbation in children with asthma. These microbes and associated cytokines may play a role in exacerbating asthma in children. John Wiley and Sons Inc. 2021-10-12 /pmc/articles/PMC8507365/ /pubmed/34667591 http://dx.doi.org/10.1002/clt2.12069 Text en © 2021 The Authors. Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kim, Yoon Hee
Jang, Haerin
Kim, Soo Yeon
Jung, Jae Hwa
Kim, Ga Eun
Park, Mi Reu
Hong, Jung Yeon
Kim, Mi Na
Kim, Eun Gyul
Kim, Min Jung
Kim, Kyung Won
Sohn, Myung Hyun
Gram‐negative microbiota is related to acute exacerbation in children with asthma
title Gram‐negative microbiota is related to acute exacerbation in children with asthma
title_full Gram‐negative microbiota is related to acute exacerbation in children with asthma
title_fullStr Gram‐negative microbiota is related to acute exacerbation in children with asthma
title_full_unstemmed Gram‐negative microbiota is related to acute exacerbation in children with asthma
title_short Gram‐negative microbiota is related to acute exacerbation in children with asthma
title_sort gram‐negative microbiota is related to acute exacerbation in children with asthma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507365/
https://www.ncbi.nlm.nih.gov/pubmed/34667591
http://dx.doi.org/10.1002/clt2.12069
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