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MicroRNA-126b-5p Exacerbates Development of Adipose Tissue and Diet-Induced Obesity

Obesity has become a worldwide epidemic, caused by many factors such as genetic regulatory elements, unhealthy diet, and lack of exercise. MicroRNAs (miRNAs) are non-coding single-stranded RNA classes, which are about 22 nucleotides in length and highly conserved among species. In the last decade, a...

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Autores principales: Shen, Linyuan, He, Jin, Zhao, Ye, Niu, Lili, Chen, Lei, Tang, Guoqing, Jiang, Yanzhi, Hao, Xiaoxia, Bai, Lin, Li, Xuewei, Zhang, Shunhua, Zhu, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508536/
https://www.ncbi.nlm.nih.gov/pubmed/34638602
http://dx.doi.org/10.3390/ijms221910261
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author Shen, Linyuan
He, Jin
Zhao, Ye
Niu, Lili
Chen, Lei
Tang, Guoqing
Jiang, Yanzhi
Hao, Xiaoxia
Bai, Lin
Li, Xuewei
Zhang, Shunhua
Zhu, Li
author_facet Shen, Linyuan
He, Jin
Zhao, Ye
Niu, Lili
Chen, Lei
Tang, Guoqing
Jiang, Yanzhi
Hao, Xiaoxia
Bai, Lin
Li, Xuewei
Zhang, Shunhua
Zhu, Li
author_sort Shen, Linyuan
collection PubMed
description Obesity has become a worldwide epidemic, caused by many factors such as genetic regulatory elements, unhealthy diet, and lack of exercise. MicroRNAs (miRNAs) are non-coding single-stranded RNA classes, which are about 22 nucleotides in length and highly conserved among species. In the last decade, a series of miRNAs were identified as therapeutic targets for obesity. In the present study, we found that miR-126b-5p was associated with adipogenesis. miR-126b-5p overexpression promoted the proliferation of 3T3-L1 preadipocytes by upregulating the expression of proliferation-related genes and downregulating the expression of apoptosis-related genes; the inhibition of miR-126b-5p gave rise to opposite results. Similarly, miR-126b-5p overexpression could promote the differentiation of 3T3-L1 preadipocytes by increasing the expression of lipid deposition genes and triglyceride (TG) and total cholesterol (TC) levels. Moreover, luciferase reporter assay demonstrated that adiponectin receptor 2 (Adipor2) and acyl-CoA dehydrogenase, long chain (ACADL) were the direct target genes of miR-126b-5p. Moreover, overexpression of miR-126b-5p could exacerbate the clinical symptoms of obesity when mice were induced by a high-fat diet, including increased adipose tissue weight, adipocyte volume, and insulin resistance. Interestingly, overexpression of miR-126b-5p in preadipocytes and mice could significantly increase total fatty acid content and change the fatty acid composition of adipose tissue. Taken together, the present study showed that miR-126b-5p promotes lipid deposition in vivo and in vitro, indicating that miR-126b-5p is a potential target for treating obesity.
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spelling pubmed-85085362021-10-13 MicroRNA-126b-5p Exacerbates Development of Adipose Tissue and Diet-Induced Obesity Shen, Linyuan He, Jin Zhao, Ye Niu, Lili Chen, Lei Tang, Guoqing Jiang, Yanzhi Hao, Xiaoxia Bai, Lin Li, Xuewei Zhang, Shunhua Zhu, Li Int J Mol Sci Article Obesity has become a worldwide epidemic, caused by many factors such as genetic regulatory elements, unhealthy diet, and lack of exercise. MicroRNAs (miRNAs) are non-coding single-stranded RNA classes, which are about 22 nucleotides in length and highly conserved among species. In the last decade, a series of miRNAs were identified as therapeutic targets for obesity. In the present study, we found that miR-126b-5p was associated with adipogenesis. miR-126b-5p overexpression promoted the proliferation of 3T3-L1 preadipocytes by upregulating the expression of proliferation-related genes and downregulating the expression of apoptosis-related genes; the inhibition of miR-126b-5p gave rise to opposite results. Similarly, miR-126b-5p overexpression could promote the differentiation of 3T3-L1 preadipocytes by increasing the expression of lipid deposition genes and triglyceride (TG) and total cholesterol (TC) levels. Moreover, luciferase reporter assay demonstrated that adiponectin receptor 2 (Adipor2) and acyl-CoA dehydrogenase, long chain (ACADL) were the direct target genes of miR-126b-5p. Moreover, overexpression of miR-126b-5p could exacerbate the clinical symptoms of obesity when mice were induced by a high-fat diet, including increased adipose tissue weight, adipocyte volume, and insulin resistance. Interestingly, overexpression of miR-126b-5p in preadipocytes and mice could significantly increase total fatty acid content and change the fatty acid composition of adipose tissue. Taken together, the present study showed that miR-126b-5p promotes lipid deposition in vivo and in vitro, indicating that miR-126b-5p is a potential target for treating obesity. MDPI 2021-09-23 /pmc/articles/PMC8508536/ /pubmed/34638602 http://dx.doi.org/10.3390/ijms221910261 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shen, Linyuan
He, Jin
Zhao, Ye
Niu, Lili
Chen, Lei
Tang, Guoqing
Jiang, Yanzhi
Hao, Xiaoxia
Bai, Lin
Li, Xuewei
Zhang, Shunhua
Zhu, Li
MicroRNA-126b-5p Exacerbates Development of Adipose Tissue and Diet-Induced Obesity
title MicroRNA-126b-5p Exacerbates Development of Adipose Tissue and Diet-Induced Obesity
title_full MicroRNA-126b-5p Exacerbates Development of Adipose Tissue and Diet-Induced Obesity
title_fullStr MicroRNA-126b-5p Exacerbates Development of Adipose Tissue and Diet-Induced Obesity
title_full_unstemmed MicroRNA-126b-5p Exacerbates Development of Adipose Tissue and Diet-Induced Obesity
title_short MicroRNA-126b-5p Exacerbates Development of Adipose Tissue and Diet-Induced Obesity
title_sort microrna-126b-5p exacerbates development of adipose tissue and diet-induced obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508536/
https://www.ncbi.nlm.nih.gov/pubmed/34638602
http://dx.doi.org/10.3390/ijms221910261
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