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Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1

Brassinosteroids (BRs) play key roles in diverse plant growth processes through a complex signaling pathway. Components orchestrating the BR signaling pathway include receptors such as kinases, transcription factors, protein kinases and phosphatases. The proper functioning of the receptor kinase BRI...

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Autores principales: Bradai, Mariem, Amorim-Silva, Vitor, Belgaroui, Nibras, Esteban del Valle, Alicia, Chabouté, Marie-Edith, Schmit, Anne-Catherine, Lozano-Duran, Rosa, Botella, Miguel Angel, Hanin, Moez, Ebel, Chantal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508605/
https://www.ncbi.nlm.nih.gov/pubmed/34638765
http://dx.doi.org/10.3390/ijms221910424
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author Bradai, Mariem
Amorim-Silva, Vitor
Belgaroui, Nibras
Esteban del Valle, Alicia
Chabouté, Marie-Edith
Schmit, Anne-Catherine
Lozano-Duran, Rosa
Botella, Miguel Angel
Hanin, Moez
Ebel, Chantal
author_facet Bradai, Mariem
Amorim-Silva, Vitor
Belgaroui, Nibras
Esteban del Valle, Alicia
Chabouté, Marie-Edith
Schmit, Anne-Catherine
Lozano-Duran, Rosa
Botella, Miguel Angel
Hanin, Moez
Ebel, Chantal
author_sort Bradai, Mariem
collection PubMed
description Brassinosteroids (BRs) play key roles in diverse plant growth processes through a complex signaling pathway. Components orchestrating the BR signaling pathway include receptors such as kinases, transcription factors, protein kinases and phosphatases. The proper functioning of the receptor kinase BRI1 and the transcription factors BES1/BZR1 depends on their dephosphorylation by type 2A protein phosphatases (PP2A). In this work, we report that an additional phosphatase family, type one protein phosphatases (PP1), contributes to the regulation of the BR signaling pathway. Co-immunoprecipitation and BiFC experiments performed in Arabidopsis plants overexpressing durum wheat TdPP1 showed that TdPP1 interacts with dephosphorylated BES1, but not with the BRI1 receptor. Higher levels of dephosphorylated, active BES1 were observed in these transgenic lines upon BR treatment, indicating that TdPP1 modifies the BR signaling pathway by activating BES1. Moreover, ectopic expression of durum wheat TdPP1 lead to an enhanced growth of primary roots in comparison to wild-type plants in presence of BR. This phenotype corroborates with a down-regulation of the BR-regulated genes CPD and DWF4. These data suggest a role of PP1 in fine-tuning BR-driven responses, most likely via the control of the phosphorylation status of BES1.
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spelling pubmed-85086052021-10-13 Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1 Bradai, Mariem Amorim-Silva, Vitor Belgaroui, Nibras Esteban del Valle, Alicia Chabouté, Marie-Edith Schmit, Anne-Catherine Lozano-Duran, Rosa Botella, Miguel Angel Hanin, Moez Ebel, Chantal Int J Mol Sci Article Brassinosteroids (BRs) play key roles in diverse plant growth processes through a complex signaling pathway. Components orchestrating the BR signaling pathway include receptors such as kinases, transcription factors, protein kinases and phosphatases. The proper functioning of the receptor kinase BRI1 and the transcription factors BES1/BZR1 depends on their dephosphorylation by type 2A protein phosphatases (PP2A). In this work, we report that an additional phosphatase family, type one protein phosphatases (PP1), contributes to the regulation of the BR signaling pathway. Co-immunoprecipitation and BiFC experiments performed in Arabidopsis plants overexpressing durum wheat TdPP1 showed that TdPP1 interacts with dephosphorylated BES1, but not with the BRI1 receptor. Higher levels of dephosphorylated, active BES1 were observed in these transgenic lines upon BR treatment, indicating that TdPP1 modifies the BR signaling pathway by activating BES1. Moreover, ectopic expression of durum wheat TdPP1 lead to an enhanced growth of primary roots in comparison to wild-type plants in presence of BR. This phenotype corroborates with a down-regulation of the BR-regulated genes CPD and DWF4. These data suggest a role of PP1 in fine-tuning BR-driven responses, most likely via the control of the phosphorylation status of BES1. MDPI 2021-09-27 /pmc/articles/PMC8508605/ /pubmed/34638765 http://dx.doi.org/10.3390/ijms221910424 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bradai, Mariem
Amorim-Silva, Vitor
Belgaroui, Nibras
Esteban del Valle, Alicia
Chabouté, Marie-Edith
Schmit, Anne-Catherine
Lozano-Duran, Rosa
Botella, Miguel Angel
Hanin, Moez
Ebel, Chantal
Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1
title Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1
title_full Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1
title_fullStr Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1
title_full_unstemmed Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1
title_short Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1
title_sort wheat type one protein phosphatase participates in the brassinosteroid control of root growth via activation of bes1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508605/
https://www.ncbi.nlm.nih.gov/pubmed/34638765
http://dx.doi.org/10.3390/ijms221910424
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