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Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (Nothobranchius guentheri)

Neurotrophins (NTs) and their signal-transducing Trk receptors play a crucial role in the development and maintenance of specific neuronal subpopulations in nervous and sensory systems. NTs are supposed to regulate two sensory systems in fish, the inner ear and the lateral line system (LLS). The lat...

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Autores principales: Aragona, Marialuisa, Porcino, Caterina, Guerrera, Maria Cristina, Montalbano, Giuseppe, Levanti, Maria, Abbate, Francesco, Laurà, Rosaria, Germanà, Antonino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508645/
https://www.ncbi.nlm.nih.gov/pubmed/34638748
http://dx.doi.org/10.3390/ijms221910411
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author Aragona, Marialuisa
Porcino, Caterina
Guerrera, Maria Cristina
Montalbano, Giuseppe
Levanti, Maria
Abbate, Francesco
Laurà, Rosaria
Germanà, Antonino
author_facet Aragona, Marialuisa
Porcino, Caterina
Guerrera, Maria Cristina
Montalbano, Giuseppe
Levanti, Maria
Abbate, Francesco
Laurà, Rosaria
Germanà, Antonino
author_sort Aragona, Marialuisa
collection PubMed
description Neurotrophins (NTs) and their signal-transducing Trk receptors play a crucial role in the development and maintenance of specific neuronal subpopulations in nervous and sensory systems. NTs are supposed to regulate two sensory systems in fish, the inner ear and the lateral line system (LLS). The latter is one of the major mechanosensory systems in fish. Considering that annual fishes of the genus Nothobranchius, with their short life expectancy, have become a suitable model for aging studies and that the occurrence and distribution of neurotrophin Trk receptors have never been investigated in the inner ear and LLS of killifish (Nothobranchius guentheri), our study aimed to investigate the localization of neurotrophin-specific Trk receptors in mechanosensory systems of N. guentheri. For histological and immunohistochemical analysis, adult specimens of N. guentheri were processed using antibodies against Trk receptors and S100 protein. An intense immunoreaction for TrkA and TrkC was found in the sensory cells of the inner ear as well as in the hair cells of LLS. Moreover, also the neurons localized in the acoustic ganglia displayed a specific immunoreaction for all Trk receptors (TrkA, B, and C) analyzed. Taken together, our results demonstrate, for the first time, that neurotrophins and their specific receptors could play a pivotal role in the biology of the sensory cells of the inner ear and LLS of N. guentheri and might also be involved in the hair cells regeneration process in normal and aged conditions.
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spelling pubmed-85086452021-10-13 Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (Nothobranchius guentheri) Aragona, Marialuisa Porcino, Caterina Guerrera, Maria Cristina Montalbano, Giuseppe Levanti, Maria Abbate, Francesco Laurà, Rosaria Germanà, Antonino Int J Mol Sci Article Neurotrophins (NTs) and their signal-transducing Trk receptors play a crucial role in the development and maintenance of specific neuronal subpopulations in nervous and sensory systems. NTs are supposed to regulate two sensory systems in fish, the inner ear and the lateral line system (LLS). The latter is one of the major mechanosensory systems in fish. Considering that annual fishes of the genus Nothobranchius, with their short life expectancy, have become a suitable model for aging studies and that the occurrence and distribution of neurotrophin Trk receptors have never been investigated in the inner ear and LLS of killifish (Nothobranchius guentheri), our study aimed to investigate the localization of neurotrophin-specific Trk receptors in mechanosensory systems of N. guentheri. For histological and immunohistochemical analysis, adult specimens of N. guentheri were processed using antibodies against Trk receptors and S100 protein. An intense immunoreaction for TrkA and TrkC was found in the sensory cells of the inner ear as well as in the hair cells of LLS. Moreover, also the neurons localized in the acoustic ganglia displayed a specific immunoreaction for all Trk receptors (TrkA, B, and C) analyzed. Taken together, our results demonstrate, for the first time, that neurotrophins and their specific receptors could play a pivotal role in the biology of the sensory cells of the inner ear and LLS of N. guentheri and might also be involved in the hair cells regeneration process in normal and aged conditions. MDPI 2021-09-27 /pmc/articles/PMC8508645/ /pubmed/34638748 http://dx.doi.org/10.3390/ijms221910411 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aragona, Marialuisa
Porcino, Caterina
Guerrera, Maria Cristina
Montalbano, Giuseppe
Levanti, Maria
Abbate, Francesco
Laurà, Rosaria
Germanà, Antonino
Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (Nothobranchius guentheri)
title Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (Nothobranchius guentheri)
title_full Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (Nothobranchius guentheri)
title_fullStr Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (Nothobranchius guentheri)
title_full_unstemmed Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (Nothobranchius guentheri)
title_short Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (Nothobranchius guentheri)
title_sort localization of neurotrophin specific trk receptors in mechanosensory systems of killifish (nothobranchius guentheri)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508645/
https://www.ncbi.nlm.nih.gov/pubmed/34638748
http://dx.doi.org/10.3390/ijms221910411
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