Cargando…
Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies
The interactions of G–quadruplexes of different topologies with highly fluorescent 9-methoxyluminarine ligand 9-MeLM were investigated by fluorescence and circular dichroism spectroscopy. The results showed that 9-methoxyluminarine was able to interact and did not destabilize any investigated molecu...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508660/ https://www.ncbi.nlm.nih.gov/pubmed/34638738 http://dx.doi.org/10.3390/ijms221910399 |
_version_ | 1784582150246367232 |
---|---|
author | Nowak-Karnowska, Joanna Głuszyńska, Agata Kosman, Joanna Neunert, Grażyna Dembska, Anna |
author_facet | Nowak-Karnowska, Joanna Głuszyńska, Agata Kosman, Joanna Neunert, Grażyna Dembska, Anna |
author_sort | Nowak-Karnowska, Joanna |
collection | PubMed |
description | The interactions of G–quadruplexes of different topologies with highly fluorescent 9-methoxyluminarine ligand 9-MeLM were investigated by fluorescence and circular dichroism spectroscopy. The results showed that 9-methoxyluminarine was able to interact and did not destabilize any investigated molecular targets. The studied compound was selectively quenched by parallel c-MYC G-quadruplex DNA, whereas hybrid and antiparallel G4 topology caused only a negligible decrease in the fluorescence of the ligand. A high decrease of fluorescence of the ligand after binding with c-MYC G-quadruplex suggests that this molecule can be used as a selective probe for parallel G-quadruplexes. |
format | Online Article Text |
id | pubmed-8508660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85086602021-10-13 Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies Nowak-Karnowska, Joanna Głuszyńska, Agata Kosman, Joanna Neunert, Grażyna Dembska, Anna Int J Mol Sci Article The interactions of G–quadruplexes of different topologies with highly fluorescent 9-methoxyluminarine ligand 9-MeLM were investigated by fluorescence and circular dichroism spectroscopy. The results showed that 9-methoxyluminarine was able to interact and did not destabilize any investigated molecular targets. The studied compound was selectively quenched by parallel c-MYC G-quadruplex DNA, whereas hybrid and antiparallel G4 topology caused only a negligible decrease in the fluorescence of the ligand. A high decrease of fluorescence of the ligand after binding with c-MYC G-quadruplex suggests that this molecule can be used as a selective probe for parallel G-quadruplexes. MDPI 2021-09-27 /pmc/articles/PMC8508660/ /pubmed/34638738 http://dx.doi.org/10.3390/ijms221910399 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nowak-Karnowska, Joanna Głuszyńska, Agata Kosman, Joanna Neunert, Grażyna Dembska, Anna Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies |
title | Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies |
title_full | Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies |
title_fullStr | Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies |
title_full_unstemmed | Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies |
title_short | Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies |
title_sort | interaction of 9-methoxyluminarine with different g-quadruplex topologies: fluorescence and circular dichroism studies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508660/ https://www.ncbi.nlm.nih.gov/pubmed/34638738 http://dx.doi.org/10.3390/ijms221910399 |
work_keys_str_mv | AT nowakkarnowskajoanna interactionof9methoxyluminarinewithdifferentgquadruplextopologiesfluorescenceandcirculardichroismstudies AT głuszynskaagata interactionof9methoxyluminarinewithdifferentgquadruplextopologiesfluorescenceandcirculardichroismstudies AT kosmanjoanna interactionof9methoxyluminarinewithdifferentgquadruplextopologiesfluorescenceandcirculardichroismstudies AT neunertgrazyna interactionof9methoxyluminarinewithdifferentgquadruplextopologiesfluorescenceandcirculardichroismstudies AT dembskaanna interactionof9methoxyluminarinewithdifferentgquadruplextopologiesfluorescenceandcirculardichroismstudies |