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The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction

The latest vaccination campaign has actualized the potential impact of antigenic stimuli on reproductive functions. To address this, we mimicked vaccination’s effects by administering keyhole limpet hemocyanin (KLH ) to CD1 male mice and used their sperm for in vitro fertilization (IVF). Two-cell em...

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Autores principales: Kontsevaya, Galina Vladimirovna, Gerlinskaya, Ludmila Alekseevna, Moshkin, Yury Mikhailovich, Anisimova, Margarita Vladimirovna, Stanova, Aliya Konstantinovna, Babochkina, Tatyana Ivanovna, Moshkin, Mikhail Pavlovich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508670/
https://www.ncbi.nlm.nih.gov/pubmed/34638989
http://dx.doi.org/10.3390/ijms221910650
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author Kontsevaya, Galina Vladimirovna
Gerlinskaya, Ludmila Alekseevna
Moshkin, Yury Mikhailovich
Anisimova, Margarita Vladimirovna
Stanova, Aliya Konstantinovna
Babochkina, Tatyana Ivanovna
Moshkin, Mikhail Pavlovich
author_facet Kontsevaya, Galina Vladimirovna
Gerlinskaya, Ludmila Alekseevna
Moshkin, Yury Mikhailovich
Anisimova, Margarita Vladimirovna
Stanova, Aliya Konstantinovna
Babochkina, Tatyana Ivanovna
Moshkin, Mikhail Pavlovich
author_sort Kontsevaya, Galina Vladimirovna
collection PubMed
description The latest vaccination campaign has actualized the potential impact of antigenic stimuli on reproductive functions. To address this, we mimicked vaccination’s effects by administering keyhole limpet hemocyanin (KLH ) to CD1 male mice and used their sperm for in vitro fertilization (IVF). Two-cell embryos after IVF with spermatozoa from control (C) or KLH-treated (Im) male mice were transferred to surrogate mothers mated with vasectomized control (C) or KLH-treated (Im) male mice, resulting in four experimental groups: C–C, Im–C, C–Im, and Im–Im. The pre-implantation losses were significantly lower in the Im–C group than in the C–Im group. At the same time, the resorption rates reduced markedly in the C–Im compared to the Im–C group. Embryo and placenta weights were significantly higher in the Im–Im group. Although the GM-CSF levels were lower in the amniotic fluid of the gestating surrogate mothers in the Im–Im group, they were strongly correlated with embryo mass. The number–size trade-off was only significant in the Im–Im group. This suggests a positive, cooperative effect of spermatozoa and seminal fluid from immune-primed males on embryo growth and the optimal distribution of surrogate mother maternal resources despite the negative impact of males’ antigenic challenge on the IVF success rate.
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spelling pubmed-85086702021-10-13 The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction Kontsevaya, Galina Vladimirovna Gerlinskaya, Ludmila Alekseevna Moshkin, Yury Mikhailovich Anisimova, Margarita Vladimirovna Stanova, Aliya Konstantinovna Babochkina, Tatyana Ivanovna Moshkin, Mikhail Pavlovich Int J Mol Sci Article The latest vaccination campaign has actualized the potential impact of antigenic stimuli on reproductive functions. To address this, we mimicked vaccination’s effects by administering keyhole limpet hemocyanin (KLH ) to CD1 male mice and used their sperm for in vitro fertilization (IVF). Two-cell embryos after IVF with spermatozoa from control (C) or KLH-treated (Im) male mice were transferred to surrogate mothers mated with vasectomized control (C) or KLH-treated (Im) male mice, resulting in four experimental groups: C–C, Im–C, C–Im, and Im–Im. The pre-implantation losses were significantly lower in the Im–C group than in the C–Im group. At the same time, the resorption rates reduced markedly in the C–Im compared to the Im–C group. Embryo and placenta weights were significantly higher in the Im–Im group. Although the GM-CSF levels were lower in the amniotic fluid of the gestating surrogate mothers in the Im–Im group, they were strongly correlated with embryo mass. The number–size trade-off was only significant in the Im–Im group. This suggests a positive, cooperative effect of spermatozoa and seminal fluid from immune-primed males on embryo growth and the optimal distribution of surrogate mother maternal resources despite the negative impact of males’ antigenic challenge on the IVF success rate. MDPI 2021-09-30 /pmc/articles/PMC8508670/ /pubmed/34638989 http://dx.doi.org/10.3390/ijms221910650 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kontsevaya, Galina Vladimirovna
Gerlinskaya, Ludmila Alekseevna
Moshkin, Yury Mikhailovich
Anisimova, Margarita Vladimirovna
Stanova, Aliya Konstantinovna
Babochkina, Tatyana Ivanovna
Moshkin, Mikhail Pavlovich
The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction
title The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction
title_full The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction
title_fullStr The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction
title_full_unstemmed The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction
title_short The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction
title_sort effects of sperm and seminal fluid of immunized male mice on in vitro fertilization and surrogate mother–embryo interaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508670/
https://www.ncbi.nlm.nih.gov/pubmed/34638989
http://dx.doi.org/10.3390/ijms221910650
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