Cargando…
Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells
Once weak ultraviolet ray-B (UVB) irradiates the skin cells, the generation of reactive nitrogen species (RNS), but not reactive oxygen species (ROS), is stimulated for the mislocalization of claudin-1 (CLDN1), an essential protein for forming tight junctions (TJs). Since our skin is constantly expo...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508977/ https://www.ncbi.nlm.nih.gov/pubmed/34638666 http://dx.doi.org/10.3390/ijms221910326 |
_version_ | 1784582225424023552 |
---|---|
author | Yoshino, Yuta Marunaka, Kana Kobayashi, Mao Matsunaga, Haruka Shu, Shokoku Matsunaga, Toshiyuki Ikari, Akira |
author_facet | Yoshino, Yuta Marunaka, Kana Kobayashi, Mao Matsunaga, Haruka Shu, Shokoku Matsunaga, Toshiyuki Ikari, Akira |
author_sort | Yoshino, Yuta |
collection | PubMed |
description | Once weak ultraviolet ray-B (UVB) irradiates the skin cells, the generation of reactive nitrogen species (RNS), but not reactive oxygen species (ROS), is stimulated for the mislocalization of claudin-1 (CLDN1), an essential protein for forming tight junctions (TJs). Since our skin is constantly exposed to sunlight throughout our lives, an effective protection strategy is needed to maintain the skin barrier against weak UVB. In the present study, we investigated whether an ethanol extract of Brazilian green propolis (EBGP) and flavonoids had a protective effect against weak UVB irradiation-induced barrier dysfunction in human keratinocyte-derived HaCaT cells. A pretreatment with EBGP suppressed TJ permeability, RNS production, and the nitration level of CLDN1 in the weak UVB-exposed cells. Among the propolis components, apigenin and apigenin-like flavonoids have potent protective effects against NO production and the mislocalization of CLDN1 induced by UVB. The analyses between structures and biological function revealed that the chemically and structurally characteristic flavonoids with a hydroxyl group at the 4′ position on the B-ring might contribute to its protective effect on barrier dysfunction caused by weak UVB irradiation. In conclusion, EBGP and its component apigenin protect HaCaT cells from weak UVB irradiation-induced TJ barrier dysfunction mediated by suppressing NO production. |
format | Online Article Text |
id | pubmed-8508977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85089772021-10-13 Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells Yoshino, Yuta Marunaka, Kana Kobayashi, Mao Matsunaga, Haruka Shu, Shokoku Matsunaga, Toshiyuki Ikari, Akira Int J Mol Sci Article Once weak ultraviolet ray-B (UVB) irradiates the skin cells, the generation of reactive nitrogen species (RNS), but not reactive oxygen species (ROS), is stimulated for the mislocalization of claudin-1 (CLDN1), an essential protein for forming tight junctions (TJs). Since our skin is constantly exposed to sunlight throughout our lives, an effective protection strategy is needed to maintain the skin barrier against weak UVB. In the present study, we investigated whether an ethanol extract of Brazilian green propolis (EBGP) and flavonoids had a protective effect against weak UVB irradiation-induced barrier dysfunction in human keratinocyte-derived HaCaT cells. A pretreatment with EBGP suppressed TJ permeability, RNS production, and the nitration level of CLDN1 in the weak UVB-exposed cells. Among the propolis components, apigenin and apigenin-like flavonoids have potent protective effects against NO production and the mislocalization of CLDN1 induced by UVB. The analyses between structures and biological function revealed that the chemically and structurally characteristic flavonoids with a hydroxyl group at the 4′ position on the B-ring might contribute to its protective effect on barrier dysfunction caused by weak UVB irradiation. In conclusion, EBGP and its component apigenin protect HaCaT cells from weak UVB irradiation-induced TJ barrier dysfunction mediated by suppressing NO production. MDPI 2021-09-25 /pmc/articles/PMC8508977/ /pubmed/34638666 http://dx.doi.org/10.3390/ijms221910326 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yoshino, Yuta Marunaka, Kana Kobayashi, Mao Matsunaga, Haruka Shu, Shokoku Matsunaga, Toshiyuki Ikari, Akira Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells |
title | Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells |
title_full | Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells |
title_fullStr | Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells |
title_full_unstemmed | Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells |
title_short | Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells |
title_sort | protective effects of ethanol extract of brazilian green propolis and apigenin against weak ultraviolet ray-b-induced barrier dysfunction via suppressing nitric oxide production and mislocalization of claudin-1 in hacat cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508977/ https://www.ncbi.nlm.nih.gov/pubmed/34638666 http://dx.doi.org/10.3390/ijms221910326 |
work_keys_str_mv | AT yoshinoyuta protectiveeffectsofethanolextractofbraziliangreenpropolisandapigeninagainstweakultravioletraybinducedbarrierdysfunctionviasuppressingnitricoxideproductionandmislocalizationofclaudin1inhacatcells AT marunakakana protectiveeffectsofethanolextractofbraziliangreenpropolisandapigeninagainstweakultravioletraybinducedbarrierdysfunctionviasuppressingnitricoxideproductionandmislocalizationofclaudin1inhacatcells AT kobayashimao protectiveeffectsofethanolextractofbraziliangreenpropolisandapigeninagainstweakultravioletraybinducedbarrierdysfunctionviasuppressingnitricoxideproductionandmislocalizationofclaudin1inhacatcells AT matsunagaharuka protectiveeffectsofethanolextractofbraziliangreenpropolisandapigeninagainstweakultravioletraybinducedbarrierdysfunctionviasuppressingnitricoxideproductionandmislocalizationofclaudin1inhacatcells AT shushokoku protectiveeffectsofethanolextractofbraziliangreenpropolisandapigeninagainstweakultravioletraybinducedbarrierdysfunctionviasuppressingnitricoxideproductionandmislocalizationofclaudin1inhacatcells AT matsunagatoshiyuki protectiveeffectsofethanolextractofbraziliangreenpropolisandapigeninagainstweakultravioletraybinducedbarrierdysfunctionviasuppressingnitricoxideproductionandmislocalizationofclaudin1inhacatcells AT ikariakira protectiveeffectsofethanolextractofbraziliangreenpropolisandapigeninagainstweakultravioletraybinducedbarrierdysfunctionviasuppressingnitricoxideproductionandmislocalizationofclaudin1inhacatcells |