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Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites
Extracellular vesicles (EVs) carry important biomolecules, including metabolites, and contribute to the spread and pathogenesis of some viruses. However, to date, limited data are available on EV metabolite content that might play a crucial role during infection with the SARS-CoV-2 virus. Therefore,...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509011/ https://www.ncbi.nlm.nih.gov/pubmed/34638812 http://dx.doi.org/10.3390/ijms221910467 |
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author | Alzahrani, Faisal A. Shait Mohammed, Mohammed Razeeth Alkarim, Saleh Azhar, Esam I. El-Magd, Mohammed A. Hawsawi, Yousef Abdulaal, Wesam H. Yusuf, Abdulaziz Alhatmi, Abdulaziz Albiheyri, Raed Fakhurji, Burhan Kurdi, Bassem Madani, Tariq A. Alguridi, Hassan Alosaimi, Roaa S. Khan, Mohammad Imran |
author_facet | Alzahrani, Faisal A. Shait Mohammed, Mohammed Razeeth Alkarim, Saleh Azhar, Esam I. El-Magd, Mohammed A. Hawsawi, Yousef Abdulaal, Wesam H. Yusuf, Abdulaziz Alhatmi, Abdulaziz Albiheyri, Raed Fakhurji, Burhan Kurdi, Bassem Madani, Tariq A. Alguridi, Hassan Alosaimi, Roaa S. Khan, Mohammad Imran |
author_sort | Alzahrani, Faisal A. |
collection | PubMed |
description | Extracellular vesicles (EVs) carry important biomolecules, including metabolites, and contribute to the spread and pathogenesis of some viruses. However, to date, limited data are available on EV metabolite content that might play a crucial role during infection with the SARS-CoV-2 virus. Therefore, this study aimed to perform untargeted metabolomics to identify key metabolites and associated pathways that are present in EVs, isolated from the serum of COVID-19 patients. The results showed the presence of antivirals and antibiotics such as Foscarnet, Indinavir, and lymecycline in EVs from patients treated with these drugs. Moreover, increased levels of anti-inflammatory metabolites such as LysoPS, 7-α,25-Dihydroxycholesterol, and 15-d-PGJ2 were detected in EVs from COVID-19 patients when compared with controls. Further, we found decreased levels of metabolites associated with coagulation, such as thromboxane and elaidic acid, in EVs from COVID-19 patients. These findings suggest that EVs not only carry active drug molecules but also anti-inflammatory metabolites, clearly suggesting that exosomes might play a crucial role in negotiating with heightened inflammation during COVID-19 infection. These preliminary results could also pave the way for the identification of novel metabolites that might act as critical regulators of inflammatory pathways during viral infections. |
format | Online Article Text |
id | pubmed-8509011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85090112021-10-13 Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites Alzahrani, Faisal A. Shait Mohammed, Mohammed Razeeth Alkarim, Saleh Azhar, Esam I. El-Magd, Mohammed A. Hawsawi, Yousef Abdulaal, Wesam H. Yusuf, Abdulaziz Alhatmi, Abdulaziz Albiheyri, Raed Fakhurji, Burhan Kurdi, Bassem Madani, Tariq A. Alguridi, Hassan Alosaimi, Roaa S. Khan, Mohammad Imran Int J Mol Sci Article Extracellular vesicles (EVs) carry important biomolecules, including metabolites, and contribute to the spread and pathogenesis of some viruses. However, to date, limited data are available on EV metabolite content that might play a crucial role during infection with the SARS-CoV-2 virus. Therefore, this study aimed to perform untargeted metabolomics to identify key metabolites and associated pathways that are present in EVs, isolated from the serum of COVID-19 patients. The results showed the presence of antivirals and antibiotics such as Foscarnet, Indinavir, and lymecycline in EVs from patients treated with these drugs. Moreover, increased levels of anti-inflammatory metabolites such as LysoPS, 7-α,25-Dihydroxycholesterol, and 15-d-PGJ2 were detected in EVs from COVID-19 patients when compared with controls. Further, we found decreased levels of metabolites associated with coagulation, such as thromboxane and elaidic acid, in EVs from COVID-19 patients. These findings suggest that EVs not only carry active drug molecules but also anti-inflammatory metabolites, clearly suggesting that exosomes might play a crucial role in negotiating with heightened inflammation during COVID-19 infection. These preliminary results could also pave the way for the identification of novel metabolites that might act as critical regulators of inflammatory pathways during viral infections. MDPI 2021-09-28 /pmc/articles/PMC8509011/ /pubmed/34638812 http://dx.doi.org/10.3390/ijms221910467 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alzahrani, Faisal A. Shait Mohammed, Mohammed Razeeth Alkarim, Saleh Azhar, Esam I. El-Magd, Mohammed A. Hawsawi, Yousef Abdulaal, Wesam H. Yusuf, Abdulaziz Alhatmi, Abdulaziz Albiheyri, Raed Fakhurji, Burhan Kurdi, Bassem Madani, Tariq A. Alguridi, Hassan Alosaimi, Roaa S. Khan, Mohammad Imran Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites |
title | Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites |
title_full | Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites |
title_fullStr | Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites |
title_full_unstemmed | Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites |
title_short | Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites |
title_sort | untargeted metabolic profiling of extracellular vesicles of sars-cov-2-infected patients shows presence of potent anti-inflammatory metabolites |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509011/ https://www.ncbi.nlm.nih.gov/pubmed/34638812 http://dx.doi.org/10.3390/ijms221910467 |
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