Cargando…

Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma

The growing application of materials containing TiO(2) particles has led to an increased risk of human exposure, while a gap in knowledge about the possible adverse effects of TiO(2) still exists. In this work, TiO(2) particles of rutile, anatase, and their commercial mixture were exposed to various...

Descripción completa

Detalles Bibliográficos
Autores principales: Korábková, Eva, Kašpárková, Věra, Jasenská, Daniela, Moricová, Dita, Daďová, Eliška, Truong, Thanh Huong, Capáková, Zdenka, Vícha, Jan, Pelková, Jana, Humpolíček, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509028/
https://www.ncbi.nlm.nih.gov/pubmed/34638952
http://dx.doi.org/10.3390/ijms221910614
_version_ 1784582237963943936
author Korábková, Eva
Kašpárková, Věra
Jasenská, Daniela
Moricová, Dita
Daďová, Eliška
Truong, Thanh Huong
Capáková, Zdenka
Vícha, Jan
Pelková, Jana
Humpolíček, Petr
author_facet Korábková, Eva
Kašpárková, Věra
Jasenská, Daniela
Moricová, Dita
Daďová, Eliška
Truong, Thanh Huong
Capáková, Zdenka
Vícha, Jan
Pelková, Jana
Humpolíček, Petr
author_sort Korábková, Eva
collection PubMed
description The growing application of materials containing TiO(2) particles has led to an increased risk of human exposure, while a gap in knowledge about the possible adverse effects of TiO(2) still exists. In this work, TiO(2) particles of rutile, anatase, and their commercial mixture were exposed to various environments, including simulated gastric fluids and human blood plasma (both representing in vivo conditions), and media used in in vitro experiments. Simulated body fluids of different compositions, ionic strengths, and pH were used, and the impact of the absence or presence of chosen enzymes was investigated. The physicochemical properties and agglomeration of TiO(2) in these media were determined. The time dependent agglomeration of TiO(2) related to the type of TiO(2), and mainly to the type and composition of the environment that was observed. The presence of enzymes either prevented or promoted TiO(2) agglomeration. TiO(2) was also observed to exhibit concentration-dependent cytotoxicity. This knowledge about TiO(2) behavior in all the abovementioned environments is critical when TiO(2) safety is considered, especially with respect to the significant impact of the presence of proteins and size-related cytotoxicity.
format Online
Article
Text
id pubmed-8509028
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85090282021-10-13 Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma Korábková, Eva Kašpárková, Věra Jasenská, Daniela Moricová, Dita Daďová, Eliška Truong, Thanh Huong Capáková, Zdenka Vícha, Jan Pelková, Jana Humpolíček, Petr Int J Mol Sci Article The growing application of materials containing TiO(2) particles has led to an increased risk of human exposure, while a gap in knowledge about the possible adverse effects of TiO(2) still exists. In this work, TiO(2) particles of rutile, anatase, and their commercial mixture were exposed to various environments, including simulated gastric fluids and human blood plasma (both representing in vivo conditions), and media used in in vitro experiments. Simulated body fluids of different compositions, ionic strengths, and pH were used, and the impact of the absence or presence of chosen enzymes was investigated. The physicochemical properties and agglomeration of TiO(2) in these media were determined. The time dependent agglomeration of TiO(2) related to the type of TiO(2), and mainly to the type and composition of the environment that was observed. The presence of enzymes either prevented or promoted TiO(2) agglomeration. TiO(2) was also observed to exhibit concentration-dependent cytotoxicity. This knowledge about TiO(2) behavior in all the abovementioned environments is critical when TiO(2) safety is considered, especially with respect to the significant impact of the presence of proteins and size-related cytotoxicity. MDPI 2021-09-30 /pmc/articles/PMC8509028/ /pubmed/34638952 http://dx.doi.org/10.3390/ijms221910614 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Korábková, Eva
Kašpárková, Věra
Jasenská, Daniela
Moricová, Dita
Daďová, Eliška
Truong, Thanh Huong
Capáková, Zdenka
Vícha, Jan
Pelková, Jana
Humpolíček, Petr
Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma
title Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma
title_full Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma
title_fullStr Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma
title_full_unstemmed Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma
title_short Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma
title_sort behaviour of titanium dioxide particles in artificial body fluids and human blood plasma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509028/
https://www.ncbi.nlm.nih.gov/pubmed/34638952
http://dx.doi.org/10.3390/ijms221910614
work_keys_str_mv AT korabkovaeva behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT kasparkovavera behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT jasenskadaniela behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT moricovadita behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT dadovaeliska behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT truongthanhhuong behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT capakovazdenka behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT vichajan behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT pelkovajana behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma
AT humpolicekpetr behaviouroftitaniumdioxideparticlesinartificialbodyfluidsandhumanbloodplasma