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Hyaluronic Acid—Dexamethasone Nanoparticles for Local Adjunct Therapy of Lung Inflammation

The delivery of a dexamethasone formulation directly into the lung appears as an appropriate strategy to strengthen the systemic administration, reducing the dosage in the treatment of lung severe inflammations. For this purpose, a hyaluronic acid-dexamethasone formulation was developed, affording a...

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Autores principales: Camara, Candelaria Ines, Bertocchi, Laura, Ricci, Caterina, Bassi, Rosaria, Bianchera, Annalisa, Cantu’, Laura, Bettini, Ruggero, Del Favero, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509068/
https://www.ncbi.nlm.nih.gov/pubmed/34638821
http://dx.doi.org/10.3390/ijms221910480
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author Camara, Candelaria Ines
Bertocchi, Laura
Ricci, Caterina
Bassi, Rosaria
Bianchera, Annalisa
Cantu’, Laura
Bettini, Ruggero
Del Favero, Elena
author_facet Camara, Candelaria Ines
Bertocchi, Laura
Ricci, Caterina
Bassi, Rosaria
Bianchera, Annalisa
Cantu’, Laura
Bettini, Ruggero
Del Favero, Elena
author_sort Camara, Candelaria Ines
collection PubMed
description The delivery of a dexamethasone formulation directly into the lung appears as an appropriate strategy to strengthen the systemic administration, reducing the dosage in the treatment of lung severe inflammations. For this purpose, a hyaluronic acid-dexamethasone formulation was developed, affording an inhalable reconstituted nanosuspension suitable to be aerosolized. The physico-chemical and biopharmaceutical properties of the formulation were tested: size, stability, loading of the spray-dried dry powder, reconstitution capability upon redispersion in aqueous media. Detailed structural insights on nanoparticles after reconstitution were obtained by light and X-ray scattering techniques. (1) The size of the nanoparticles, around 200 nm, is in the proper range for a possible engulfment by macrophages. (2) Their structure is of the core-shell type, hosting dexamethasone nanocrystals inside and carrying hyaluronic acid chains on the surface. This specific structure allows for nanosuspension stability and provides nanoparticles with muco-inert properties. (3) The nanosuspension can be efficiently aerosolized, allowing for a high drug fraction potentially reaching the deep lung. Thus, this formulation represents a promising tool for the lung administration via nebulization directly in the pipe of ventilators, to be used as such or as adjunct therapy for severe lung inflammation.
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spelling pubmed-85090682021-10-13 Hyaluronic Acid—Dexamethasone Nanoparticles for Local Adjunct Therapy of Lung Inflammation Camara, Candelaria Ines Bertocchi, Laura Ricci, Caterina Bassi, Rosaria Bianchera, Annalisa Cantu’, Laura Bettini, Ruggero Del Favero, Elena Int J Mol Sci Article The delivery of a dexamethasone formulation directly into the lung appears as an appropriate strategy to strengthen the systemic administration, reducing the dosage in the treatment of lung severe inflammations. For this purpose, a hyaluronic acid-dexamethasone formulation was developed, affording an inhalable reconstituted nanosuspension suitable to be aerosolized. The physico-chemical and biopharmaceutical properties of the formulation were tested: size, stability, loading of the spray-dried dry powder, reconstitution capability upon redispersion in aqueous media. Detailed structural insights on nanoparticles after reconstitution were obtained by light and X-ray scattering techniques. (1) The size of the nanoparticles, around 200 nm, is in the proper range for a possible engulfment by macrophages. (2) Their structure is of the core-shell type, hosting dexamethasone nanocrystals inside and carrying hyaluronic acid chains on the surface. This specific structure allows for nanosuspension stability and provides nanoparticles with muco-inert properties. (3) The nanosuspension can be efficiently aerosolized, allowing for a high drug fraction potentially reaching the deep lung. Thus, this formulation represents a promising tool for the lung administration via nebulization directly in the pipe of ventilators, to be used as such or as adjunct therapy for severe lung inflammation. MDPI 2021-09-28 /pmc/articles/PMC8509068/ /pubmed/34638821 http://dx.doi.org/10.3390/ijms221910480 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Camara, Candelaria Ines
Bertocchi, Laura
Ricci, Caterina
Bassi, Rosaria
Bianchera, Annalisa
Cantu’, Laura
Bettini, Ruggero
Del Favero, Elena
Hyaluronic Acid—Dexamethasone Nanoparticles for Local Adjunct Therapy of Lung Inflammation
title Hyaluronic Acid—Dexamethasone Nanoparticles for Local Adjunct Therapy of Lung Inflammation
title_full Hyaluronic Acid—Dexamethasone Nanoparticles for Local Adjunct Therapy of Lung Inflammation
title_fullStr Hyaluronic Acid—Dexamethasone Nanoparticles for Local Adjunct Therapy of Lung Inflammation
title_full_unstemmed Hyaluronic Acid—Dexamethasone Nanoparticles for Local Adjunct Therapy of Lung Inflammation
title_short Hyaluronic Acid—Dexamethasone Nanoparticles for Local Adjunct Therapy of Lung Inflammation
title_sort hyaluronic acid—dexamethasone nanoparticles for local adjunct therapy of lung inflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509068/
https://www.ncbi.nlm.nih.gov/pubmed/34638821
http://dx.doi.org/10.3390/ijms221910480
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