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Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants
Agrobacterium-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an Agrobacterium-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509792/ https://www.ncbi.nlm.nih.gov/pubmed/34639221 http://dx.doi.org/10.3390/ijms221910882 |
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author | Kaur, Maninder Manchanda, Pooja Kalia, Anu Ahmed, Farah K. Nepovimova, Eugenie Kuca, Kamil Abd-Elsalam, Kamel A. |
author_facet | Kaur, Maninder Manchanda, Pooja Kalia, Anu Ahmed, Farah K. Nepovimova, Eugenie Kuca, Kamil Abd-Elsalam, Kamel A. |
author_sort | Kaur, Maninder |
collection | PubMed |
description | Agrobacterium-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an Agrobacterium-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providing a large picture through pilot-scale experiment via transient approach. The direct delivery of recombinant agrobacteria through this approach carrying CRISPR/Cas cassette to knockout the expression of the target gene in the intercellular tissue spaces by physical or vacuum infiltration can simplify the targeted site modification. This review aims to provide information on Agrobacterium-mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. This will ease the screening of numerous functions of genes in different plant species with wider applicability in future. |
format | Online Article Text |
id | pubmed-8509792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85097922021-10-13 Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants Kaur, Maninder Manchanda, Pooja Kalia, Anu Ahmed, Farah K. Nepovimova, Eugenie Kuca, Kamil Abd-Elsalam, Kamel A. Int J Mol Sci Review Agrobacterium-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an Agrobacterium-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providing a large picture through pilot-scale experiment via transient approach. The direct delivery of recombinant agrobacteria through this approach carrying CRISPR/Cas cassette to knockout the expression of the target gene in the intercellular tissue spaces by physical or vacuum infiltration can simplify the targeted site modification. This review aims to provide information on Agrobacterium-mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. This will ease the screening of numerous functions of genes in different plant species with wider applicability in future. MDPI 2021-10-08 /pmc/articles/PMC8509792/ /pubmed/34639221 http://dx.doi.org/10.3390/ijms221910882 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Kaur, Maninder Manchanda, Pooja Kalia, Anu Ahmed, Farah K. Nepovimova, Eugenie Kuca, Kamil Abd-Elsalam, Kamel A. Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
title | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
title_full | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
title_fullStr | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
title_full_unstemmed | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
title_short | Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants |
title_sort | agroinfiltration mediated scalable transient gene expression in genome edited crop plants |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509792/ https://www.ncbi.nlm.nih.gov/pubmed/34639221 http://dx.doi.org/10.3390/ijms221910882 |
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