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High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks

Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammal...

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Autores principales: Mbehang Nguema, Pierre Philippe, Onanga, Richard, Ndong Atome, Guy Roger, Tewa, Jean Jules, Mabika Mabika, Arsène, Muandze Nzambe, Jean Ulrich, Obague Mbeang, Jean Constant, Bitome Essono, Paul Yannick, Bretagnolle, François, Godreuil, Sylvain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509864/
https://www.ncbi.nlm.nih.gov/pubmed/34637441
http://dx.doi.org/10.1371/journal.pone.0257994
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author Mbehang Nguema, Pierre Philippe
Onanga, Richard
Ndong Atome, Guy Roger
Tewa, Jean Jules
Mabika Mabika, Arsène
Muandze Nzambe, Jean Ulrich
Obague Mbeang, Jean Constant
Bitome Essono, Paul Yannick
Bretagnolle, François
Godreuil, Sylvain
author_facet Mbehang Nguema, Pierre Philippe
Onanga, Richard
Ndong Atome, Guy Roger
Tewa, Jean Jules
Mabika Mabika, Arsène
Muandze Nzambe, Jean Ulrich
Obague Mbeang, Jean Constant
Bitome Essono, Paul Yannick
Bretagnolle, François
Godreuil, Sylvain
author_sort Mbehang Nguema, Pierre Philippe
collection PubMed
description Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines. The preliminary test for ESBL-producing Enterobacteriaceae was performed by replicating enterobacterial colonies on MacConkey agar supplemented with 2 mg/L cefotaxime (MCA+CTX). Extended-spectrum beta-lactamase (ESBL) production was confirmed with the double-disc synergy test (DDST). The inhibition zone diameters were read with SirScan. Among the 130 bacterial colonies isolated from 125 fecal samples, 90 enterobacterial isolates were identified. Escherichia coli (61%) was the most prevalent, followed by Enterobacter cloacae (8%), Proteus mirabilis (8%), Klebsiella variicola (7%), Klebsiella aerogenes (7%), Klebsiella oxytoca (4%), Citrobacter freundii (3%), Klebsiella pneumoniae (1%) and Serratia marcescens (1%). Acquired resistance was carried by E. coli (11% of all E. coli isolates) and E. cloacae (3% of all E. cloacae) isolates, while intrinsic resistance was detected in all the other resistant isolates (n = 31); K. variicola, K. oxytoca, K. pneumoniae, E. cloacae, K. aerogenes, S. marcescens and P. mirabilis). Our data show that most strains isolated in protected areas in Gabon are wild type isolates and carry intrinsic resistance rather than acquired resistance.
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spelling pubmed-85098642021-10-13 High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks Mbehang Nguema, Pierre Philippe Onanga, Richard Ndong Atome, Guy Roger Tewa, Jean Jules Mabika Mabika, Arsène Muandze Nzambe, Jean Ulrich Obague Mbeang, Jean Constant Bitome Essono, Paul Yannick Bretagnolle, François Godreuil, Sylvain PLoS One Research Article Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines. The preliminary test for ESBL-producing Enterobacteriaceae was performed by replicating enterobacterial colonies on MacConkey agar supplemented with 2 mg/L cefotaxime (MCA+CTX). Extended-spectrum beta-lactamase (ESBL) production was confirmed with the double-disc synergy test (DDST). The inhibition zone diameters were read with SirScan. Among the 130 bacterial colonies isolated from 125 fecal samples, 90 enterobacterial isolates were identified. Escherichia coli (61%) was the most prevalent, followed by Enterobacter cloacae (8%), Proteus mirabilis (8%), Klebsiella variicola (7%), Klebsiella aerogenes (7%), Klebsiella oxytoca (4%), Citrobacter freundii (3%), Klebsiella pneumoniae (1%) and Serratia marcescens (1%). Acquired resistance was carried by E. coli (11% of all E. coli isolates) and E. cloacae (3% of all E. cloacae) isolates, while intrinsic resistance was detected in all the other resistant isolates (n = 31); K. variicola, K. oxytoca, K. pneumoniae, E. cloacae, K. aerogenes, S. marcescens and P. mirabilis). Our data show that most strains isolated in protected areas in Gabon are wild type isolates and carry intrinsic resistance rather than acquired resistance. Public Library of Science 2021-10-12 /pmc/articles/PMC8509864/ /pubmed/34637441 http://dx.doi.org/10.1371/journal.pone.0257994 Text en © 2021 Mbehang Nguema et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mbehang Nguema, Pierre Philippe
Onanga, Richard
Ndong Atome, Guy Roger
Tewa, Jean Jules
Mabika Mabika, Arsène
Muandze Nzambe, Jean Ulrich
Obague Mbeang, Jean Constant
Bitome Essono, Paul Yannick
Bretagnolle, François
Godreuil, Sylvain
High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks
title High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks
title_full High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks
title_fullStr High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks
title_full_unstemmed High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks
title_short High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks
title_sort high level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in gabonese national parks
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509864/
https://www.ncbi.nlm.nih.gov/pubmed/34637441
http://dx.doi.org/10.1371/journal.pone.0257994
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