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High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks
Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammal...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509864/ https://www.ncbi.nlm.nih.gov/pubmed/34637441 http://dx.doi.org/10.1371/journal.pone.0257994 |
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author | Mbehang Nguema, Pierre Philippe Onanga, Richard Ndong Atome, Guy Roger Tewa, Jean Jules Mabika Mabika, Arsène Muandze Nzambe, Jean Ulrich Obague Mbeang, Jean Constant Bitome Essono, Paul Yannick Bretagnolle, François Godreuil, Sylvain |
author_facet | Mbehang Nguema, Pierre Philippe Onanga, Richard Ndong Atome, Guy Roger Tewa, Jean Jules Mabika Mabika, Arsène Muandze Nzambe, Jean Ulrich Obague Mbeang, Jean Constant Bitome Essono, Paul Yannick Bretagnolle, François Godreuil, Sylvain |
author_sort | Mbehang Nguema, Pierre Philippe |
collection | PubMed |
description | Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines. The preliminary test for ESBL-producing Enterobacteriaceae was performed by replicating enterobacterial colonies on MacConkey agar supplemented with 2 mg/L cefotaxime (MCA+CTX). Extended-spectrum beta-lactamase (ESBL) production was confirmed with the double-disc synergy test (DDST). The inhibition zone diameters were read with SirScan. Among the 130 bacterial colonies isolated from 125 fecal samples, 90 enterobacterial isolates were identified. Escherichia coli (61%) was the most prevalent, followed by Enterobacter cloacae (8%), Proteus mirabilis (8%), Klebsiella variicola (7%), Klebsiella aerogenes (7%), Klebsiella oxytoca (4%), Citrobacter freundii (3%), Klebsiella pneumoniae (1%) and Serratia marcescens (1%). Acquired resistance was carried by E. coli (11% of all E. coli isolates) and E. cloacae (3% of all E. cloacae) isolates, while intrinsic resistance was detected in all the other resistant isolates (n = 31); K. variicola, K. oxytoca, K. pneumoniae, E. cloacae, K. aerogenes, S. marcescens and P. mirabilis). Our data show that most strains isolated in protected areas in Gabon are wild type isolates and carry intrinsic resistance rather than acquired resistance. |
format | Online Article Text |
id | pubmed-8509864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-85098642021-10-13 High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks Mbehang Nguema, Pierre Philippe Onanga, Richard Ndong Atome, Guy Roger Tewa, Jean Jules Mabika Mabika, Arsène Muandze Nzambe, Jean Ulrich Obague Mbeang, Jean Constant Bitome Essono, Paul Yannick Bretagnolle, François Godreuil, Sylvain PLoS One Research Article Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines. The preliminary test for ESBL-producing Enterobacteriaceae was performed by replicating enterobacterial colonies on MacConkey agar supplemented with 2 mg/L cefotaxime (MCA+CTX). Extended-spectrum beta-lactamase (ESBL) production was confirmed with the double-disc synergy test (DDST). The inhibition zone diameters were read with SirScan. Among the 130 bacterial colonies isolated from 125 fecal samples, 90 enterobacterial isolates were identified. Escherichia coli (61%) was the most prevalent, followed by Enterobacter cloacae (8%), Proteus mirabilis (8%), Klebsiella variicola (7%), Klebsiella aerogenes (7%), Klebsiella oxytoca (4%), Citrobacter freundii (3%), Klebsiella pneumoniae (1%) and Serratia marcescens (1%). Acquired resistance was carried by E. coli (11% of all E. coli isolates) and E. cloacae (3% of all E. cloacae) isolates, while intrinsic resistance was detected in all the other resistant isolates (n = 31); K. variicola, K. oxytoca, K. pneumoniae, E. cloacae, K. aerogenes, S. marcescens and P. mirabilis). Our data show that most strains isolated in protected areas in Gabon are wild type isolates and carry intrinsic resistance rather than acquired resistance. Public Library of Science 2021-10-12 /pmc/articles/PMC8509864/ /pubmed/34637441 http://dx.doi.org/10.1371/journal.pone.0257994 Text en © 2021 Mbehang Nguema et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mbehang Nguema, Pierre Philippe Onanga, Richard Ndong Atome, Guy Roger Tewa, Jean Jules Mabika Mabika, Arsène Muandze Nzambe, Jean Ulrich Obague Mbeang, Jean Constant Bitome Essono, Paul Yannick Bretagnolle, François Godreuil, Sylvain High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks |
title | High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks |
title_full | High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks |
title_fullStr | High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks |
title_full_unstemmed | High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks |
title_short | High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks |
title_sort | high level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in gabonese national parks |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509864/ https://www.ncbi.nlm.nih.gov/pubmed/34637441 http://dx.doi.org/10.1371/journal.pone.0257994 |
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