Cargando…

Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation

This paper presents an efficient method of microbial-induced calcite precipitation (MICP) for cementation of sand particles. First, the influence of initial pH value of the culture medium on the growth of bacteria was discussed. Then, the compressive strength and calcium carbonate content of cemente...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Lu, Liu, Shuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510110/
https://www.ncbi.nlm.nih.gov/pubmed/34640027
http://dx.doi.org/10.3390/ma14195631
_version_ 1784582496977944576
author Wang, Lu
Liu, Shuhua
author_facet Wang, Lu
Liu, Shuhua
author_sort Wang, Lu
collection PubMed
description This paper presents an efficient method of microbial-induced calcite precipitation (MICP) for cementation of sand particles. First, the influence of initial pH value of the culture medium on the growth of bacteria was discussed. Then, the compressive strength and calcium carbonate content of cemented sand columns with different sand particle sizes were measured to indicate the cementation effectiveness. The microstructure of cemented sand columns as well as the mineral composition and distribution of calcium carbonate were characterised by means of scanning electron microscopy-energy dispersive spectrometer (SEM-EDS) and X-ray diffraction (XRD). The results showed that the urease-producing bacteria S. pasteurii can be cultured at the initial pH values of 7–10, while a higher pH (e.g., 11) would hinder its growth and decrease its urease activity. The injection method of MICP with high standing time can cement sand columns effectively. Small average sand particle size of sand columns and high injection cycles can facilitate the gain of compressive strength, while calcium carbonate content of sand column higher than 8% can promote the increase of compressive strength. XRD results indicate that the fine grains generated on the surface of sand particles are calcite. The distribution of calcite on sand particles’ surface is broad and uniform. First, calcite was precipitated on the surface of sand particles, and then a precipitation layer was formed, which would connect sand particles through its high enough thickness and contribute to the development of compressive strength of the whole sand column.
format Online
Article
Text
id pubmed-8510110
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85101102021-10-13 Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation Wang, Lu Liu, Shuhua Materials (Basel) Article This paper presents an efficient method of microbial-induced calcite precipitation (MICP) for cementation of sand particles. First, the influence of initial pH value of the culture medium on the growth of bacteria was discussed. Then, the compressive strength and calcium carbonate content of cemented sand columns with different sand particle sizes were measured to indicate the cementation effectiveness. The microstructure of cemented sand columns as well as the mineral composition and distribution of calcium carbonate were characterised by means of scanning electron microscopy-energy dispersive spectrometer (SEM-EDS) and X-ray diffraction (XRD). The results showed that the urease-producing bacteria S. pasteurii can be cultured at the initial pH values of 7–10, while a higher pH (e.g., 11) would hinder its growth and decrease its urease activity. The injection method of MICP with high standing time can cement sand columns effectively. Small average sand particle size of sand columns and high injection cycles can facilitate the gain of compressive strength, while calcium carbonate content of sand column higher than 8% can promote the increase of compressive strength. XRD results indicate that the fine grains generated on the surface of sand particles are calcite. The distribution of calcite on sand particles’ surface is broad and uniform. First, calcite was precipitated on the surface of sand particles, and then a precipitation layer was formed, which would connect sand particles through its high enough thickness and contribute to the development of compressive strength of the whole sand column. MDPI 2021-09-28 /pmc/articles/PMC8510110/ /pubmed/34640027 http://dx.doi.org/10.3390/ma14195631 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Lu
Liu, Shuhua
Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation
title Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation
title_full Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation
title_fullStr Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation
title_full_unstemmed Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation
title_short Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation
title_sort mechanism of sand cementation with an efficient method of microbial-induced calcite precipitation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510110/
https://www.ncbi.nlm.nih.gov/pubmed/34640027
http://dx.doi.org/10.3390/ma14195631
work_keys_str_mv AT wanglu mechanismofsandcementationwithanefficientmethodofmicrobialinducedcalciteprecipitation
AT liushuhua mechanismofsandcementationwithanefficientmethodofmicrobialinducedcalciteprecipitation