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A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone
In this paper, a novel antioxidant analysis is proposed using a simple minimized device based on moving drops as solution handling and a smartphone as a detector. This approach is based on the colorimetric determination of the scavenging activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH(•...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510342/ https://www.ncbi.nlm.nih.gov/pubmed/34641288 http://dx.doi.org/10.3390/molecules26195744 |
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author | Apichai, Sutasinee Thajee, Kajorngai Pattananandecha, Thanawat Saenjum, Chalermpong Grudpan, Kate |
author_facet | Apichai, Sutasinee Thajee, Kajorngai Pattananandecha, Thanawat Saenjum, Chalermpong Grudpan, Kate |
author_sort | Apichai, Sutasinee |
collection | PubMed |
description | In this paper, a novel antioxidant analysis is proposed using a simple minimized device based on moving drops as solution handling and a smartphone as a detector. This approach is based on the colorimetric determination of the scavenging activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH(•)), expressed as the half-maximal inhibitory concentration (IC(50)), vitamin C equivalent antioxidant capacity (VCEAC), and Trolox equivalent antioxidant capacity (TEAC). A small drop of the positive control or the samples moves by eluting an ethanol drop down by the force of gravity to react with a DPPH(•) drop in the detection zone. The color change of DPPH(•) is monitored by a smartphone camera, and the color signals are processed using Adobe Photoshop software. The magenta-to-yellow ratio was successfully applied to evaluate the percentage of DPPH(•) inhibition with no significant difference compared with the reference spectrophotometric method at a confidence level of 95%. The total phenolic content (TPC) was measured using the Folin–Ciocalteu assay. An application to Miang (fermented tea leaf extract) showed the consonant relationship between the scavenging activity of DPPH(•) and TPC. |
format | Online Article Text |
id | pubmed-8510342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85103422021-10-13 A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone Apichai, Sutasinee Thajee, Kajorngai Pattananandecha, Thanawat Saenjum, Chalermpong Grudpan, Kate Molecules Article In this paper, a novel antioxidant analysis is proposed using a simple minimized device based on moving drops as solution handling and a smartphone as a detector. This approach is based on the colorimetric determination of the scavenging activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH(•)), expressed as the half-maximal inhibitory concentration (IC(50)), vitamin C equivalent antioxidant capacity (VCEAC), and Trolox equivalent antioxidant capacity (TEAC). A small drop of the positive control or the samples moves by eluting an ethanol drop down by the force of gravity to react with a DPPH(•) drop in the detection zone. The color change of DPPH(•) is monitored by a smartphone camera, and the color signals are processed using Adobe Photoshop software. The magenta-to-yellow ratio was successfully applied to evaluate the percentage of DPPH(•) inhibition with no significant difference compared with the reference spectrophotometric method at a confidence level of 95%. The total phenolic content (TPC) was measured using the Folin–Ciocalteu assay. An application to Miang (fermented tea leaf extract) showed the consonant relationship between the scavenging activity of DPPH(•) and TPC. MDPI 2021-09-22 /pmc/articles/PMC8510342/ /pubmed/34641288 http://dx.doi.org/10.3390/molecules26195744 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Apichai, Sutasinee Thajee, Kajorngai Pattananandecha, Thanawat Saenjum, Chalermpong Grudpan, Kate A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone |
title | A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone |
title_full | A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone |
title_fullStr | A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone |
title_full_unstemmed | A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone |
title_short | A Simple Minimized System Based on Moving Drops for Antioxidant Analysis Using a Smartphone |
title_sort | simple minimized system based on moving drops for antioxidant analysis using a smartphone |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510342/ https://www.ncbi.nlm.nih.gov/pubmed/34641288 http://dx.doi.org/10.3390/molecules26195744 |
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