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Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay

Salmonella species are among the major pathogens that cause foodborne illness outbreaks. In this study, we aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Salmonella species. We designed LAMP primers targeting the hilA gene as a univers...

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Autores principales: Chu, Jiyon, Shin, Juyoun, Kang, Shinseok, Shin, Sun, Chung, Yeun-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korea Genome Organization 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510866/
https://www.ncbi.nlm.nih.gov/pubmed/34638177
http://dx.doi.org/10.5808/gi.21048
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author Chu, Jiyon
Shin, Juyoun
Kang, Shinseok
Shin, Sun
Chung, Yeun-Jun
author_facet Chu, Jiyon
Shin, Juyoun
Kang, Shinseok
Shin, Sun
Chung, Yeun-Jun
author_sort Chu, Jiyon
collection PubMed
description Salmonella species are among the major pathogens that cause foodborne illness outbreaks. In this study, we aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Salmonella species. We designed LAMP primers targeting the hilA gene as a universal marker of Salmonella species. A total of seven Salmonella species strains and 11 non-Salmonella pathogen strains from eight different genera were used in this study. All Salmonella strains showed positive amplification signals with the Salmonella LAMP assay; however, there was no non-specific amplification signal for the non-Salmonella strains. The detection limit was 100 femtograms (20 copies per reaction), which was ~1,000 times more sensitive than the detection limits of the conventional polymerase chain reaction (PCR) assay (100 pg). The reaction time for a positive amplification signal was less than 20 minutes, which was less than one-third the time taken while using conventional PCR. In conclusion, our Salmonella LAMP assay accurately detected Salmonella species with a higher degree of sensitivity and greater rapidity than the conventional PCR assay, and it may be suitable for point-of-care testing in the field.
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spelling pubmed-85108662021-10-22 Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay Chu, Jiyon Shin, Juyoun Kang, Shinseok Shin, Sun Chung, Yeun-Jun Genomics Inform Original Article Salmonella species are among the major pathogens that cause foodborne illness outbreaks. In this study, we aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Salmonella species. We designed LAMP primers targeting the hilA gene as a universal marker of Salmonella species. A total of seven Salmonella species strains and 11 non-Salmonella pathogen strains from eight different genera were used in this study. All Salmonella strains showed positive amplification signals with the Salmonella LAMP assay; however, there was no non-specific amplification signal for the non-Salmonella strains. The detection limit was 100 femtograms (20 copies per reaction), which was ~1,000 times more sensitive than the detection limits of the conventional polymerase chain reaction (PCR) assay (100 pg). The reaction time for a positive amplification signal was less than 20 minutes, which was less than one-third the time taken while using conventional PCR. In conclusion, our Salmonella LAMP assay accurately detected Salmonella species with a higher degree of sensitivity and greater rapidity than the conventional PCR assay, and it may be suitable for point-of-care testing in the field. Korea Genome Organization 2021-09-30 /pmc/articles/PMC8510866/ /pubmed/34638177 http://dx.doi.org/10.5808/gi.21048 Text en (c) 2021, Korea Genome Organization https://creativecommons.org/licenses/by/4.0/(CC) This is an open-access article distributed under the terms of the Creative Commons Attribution license(https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chu, Jiyon
Shin, Juyoun
Kang, Shinseok
Shin, Sun
Chung, Yeun-Jun
Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay
title Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay
title_full Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay
title_fullStr Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay
title_full_unstemmed Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay
title_short Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay
title_sort rapid and sensitive detection of salmonella species targeting the hila gene using a loop-mediated isothermal amplification assay
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510866/
https://www.ncbi.nlm.nih.gov/pubmed/34638177
http://dx.doi.org/10.5808/gi.21048
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