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In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system

A drug delivery system based on mesoporous particles MCM-41 was post-synthetically modified by photo-sensitive ligand, methyl-(2E)-3-(4-(triethoxysilyl)-propoxyphenyl)-2-propenoate (CA) and the pores of MCM-41 particles were loaded with Naproxen sodium salt (NAP). The CA was used as a photoactive mo...

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Autores principales: Almáši, Miroslav, Matiašová, Anna Alexovič, Šuleková, Monika, Beňová, Eva, Ševc, Juraj, Váhovská, Lucia, Lisnichuk, Maksym, Girman, Vladimír, Zeleňáková, Adriana, Hudák, Alexander, Zeleňák, Vladimír
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511123/
https://www.ncbi.nlm.nih.gov/pubmed/34642409
http://dx.doi.org/10.1038/s41598-021-99678-y
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author Almáši, Miroslav
Matiašová, Anna Alexovič
Šuleková, Monika
Beňová, Eva
Ševc, Juraj
Váhovská, Lucia
Lisnichuk, Maksym
Girman, Vladimír
Zeleňáková, Adriana
Hudák, Alexander
Zeleňák, Vladimír
author_facet Almáši, Miroslav
Matiašová, Anna Alexovič
Šuleková, Monika
Beňová, Eva
Ševc, Juraj
Váhovská, Lucia
Lisnichuk, Maksym
Girman, Vladimír
Zeleňáková, Adriana
Hudák, Alexander
Zeleňák, Vladimír
author_sort Almáši, Miroslav
collection PubMed
description A drug delivery system based on mesoporous particles MCM-41 was post-synthetically modified by photo-sensitive ligand, methyl-(2E)-3-(4-(triethoxysilyl)-propoxyphenyl)-2-propenoate (CA) and the pores of MCM-41 particles were loaded with Naproxen sodium salt (NAP). The CA was used as a photoactive molecule that can undergo a reversible photo-dimerization by [2π + 2π] cycloaddition when irradiated with UV light of specific wavelengths. Thus, it has a function of gate-keeper that is responsible for opening/closing the pores and minimizing premature release of NAP. The physicochemical properties of the prepared system were studied by infrared spectroscopy (IR), nitrogen adsorption measurements, thermogravimetric analysis (TGA), scanning transmission electron microscopy (STEM) and energy dispersive X-ray spectroscopy (EDX). The mechanism of the opening/closing pores was confirmed by UV measurements. In vitro and in vivo drug release experiments and the concentration of released NAP was determined by UV spectroscopy and high-performance liquid chromatography (HPLC). In vivo drug release in the blood circulatory system of rats has demonstrated the effective photo-cleavage reaction of CA molecules after UV-light stimulation. The localization and morphological changes of the particles were studied in the blood and liver of rats at different time intervals. The particles in the blood have been shown to retain their original rod-like shape, and the particles in the liver have been hydrolysed, which has resulted in spherical shape with a reduced size.
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spelling pubmed-85111232021-10-14 In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system Almáši, Miroslav Matiašová, Anna Alexovič Šuleková, Monika Beňová, Eva Ševc, Juraj Váhovská, Lucia Lisnichuk, Maksym Girman, Vladimír Zeleňáková, Adriana Hudák, Alexander Zeleňák, Vladimír Sci Rep Article A drug delivery system based on mesoporous particles MCM-41 was post-synthetically modified by photo-sensitive ligand, methyl-(2E)-3-(4-(triethoxysilyl)-propoxyphenyl)-2-propenoate (CA) and the pores of MCM-41 particles were loaded with Naproxen sodium salt (NAP). The CA was used as a photoactive molecule that can undergo a reversible photo-dimerization by [2π + 2π] cycloaddition when irradiated with UV light of specific wavelengths. Thus, it has a function of gate-keeper that is responsible for opening/closing the pores and minimizing premature release of NAP. The physicochemical properties of the prepared system were studied by infrared spectroscopy (IR), nitrogen adsorption measurements, thermogravimetric analysis (TGA), scanning transmission electron microscopy (STEM) and energy dispersive X-ray spectroscopy (EDX). The mechanism of the opening/closing pores was confirmed by UV measurements. In vitro and in vivo drug release experiments and the concentration of released NAP was determined by UV spectroscopy and high-performance liquid chromatography (HPLC). In vivo drug release in the blood circulatory system of rats has demonstrated the effective photo-cleavage reaction of CA molecules after UV-light stimulation. The localization and morphological changes of the particles were studied in the blood and liver of rats at different time intervals. The particles in the blood have been shown to retain their original rod-like shape, and the particles in the liver have been hydrolysed, which has resulted in spherical shape with a reduced size. Nature Publishing Group UK 2021-10-12 /pmc/articles/PMC8511123/ /pubmed/34642409 http://dx.doi.org/10.1038/s41598-021-99678-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Almáši, Miroslav
Matiašová, Anna Alexovič
Šuleková, Monika
Beňová, Eva
Ševc, Juraj
Váhovská, Lucia
Lisnichuk, Maksym
Girman, Vladimír
Zeleňáková, Adriana
Hudák, Alexander
Zeleňák, Vladimír
In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system
title In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system
title_full In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system
title_fullStr In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system
title_full_unstemmed In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system
title_short In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system
title_sort in vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511123/
https://www.ncbi.nlm.nih.gov/pubmed/34642409
http://dx.doi.org/10.1038/s41598-021-99678-y
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