The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice

Adjuvants are often required to improve the potentially low immunogenicity of vaccines. In this study, it is proposed to use an emulsion based on fluorocarbons as an adjuvant. Since this emulsion adsorbs only a small range of proteins, apolipoprotein A-I (ApoAI) was used as an anchor. Antigen and Ap...

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Autores principales: Zhalimov, Vitaly, Gritsyna, Yuliya, Morenkov, Oleg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Vaccine Society 2021
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511586/
https://www.ncbi.nlm.nih.gov/pubmed/34703809
http://dx.doi.org/10.7774/cevr.2021.10.3.259
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author Zhalimov, Vitaly
Gritsyna, Yuliya
Morenkov, Oleg
author_facet Zhalimov, Vitaly
Gritsyna, Yuliya
Morenkov, Oleg
author_sort Zhalimov, Vitaly
collection PubMed
description Adjuvants are often required to improve the potentially low immunogenicity of vaccines. In this study, it is proposed to use an emulsion based on fluorocarbons as an adjuvant. Since this emulsion adsorbs only a small range of proteins, apolipoprotein A-I (ApoAI) was used as an anchor. Antigen and ApoAI were combined by creating a fusion construct. Results showed that the combined use of a perfluorocarbon emulsion and ApoAI during immunization significantly increases the specific antibody titer in mice and in its effectiveness this system is close to the incomplete Freund's adjuvant.
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spelling pubmed-85115862021-10-25 The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice Zhalimov, Vitaly Gritsyna, Yuliya Morenkov, Oleg Clin Exp Vaccine Res Brief Communication Adjuvants are often required to improve the potentially low immunogenicity of vaccines. In this study, it is proposed to use an emulsion based on fluorocarbons as an adjuvant. Since this emulsion adsorbs only a small range of proteins, apolipoprotein A-I (ApoAI) was used as an anchor. Antigen and ApoAI were combined by creating a fusion construct. Results showed that the combined use of a perfluorocarbon emulsion and ApoAI during immunization significantly increases the specific antibody titer in mice and in its effectiveness this system is close to the incomplete Freund's adjuvant. The Korean Vaccine Society 2021-09 2021-09-30 /pmc/articles/PMC8511586/ /pubmed/34703809 http://dx.doi.org/10.7774/cevr.2021.10.3.259 Text en © Korean Vaccine Society. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Zhalimov, Vitaly
Gritsyna, Yuliya
Morenkov, Oleg
The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice
title The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice
title_full The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice
title_fullStr The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice
title_full_unstemmed The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice
title_short The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice
title_sort effect of apolipoprotein a-i and perfluorocarbon emulsion on the production of specific antibodies in mice
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511586/
https://www.ncbi.nlm.nih.gov/pubmed/34703809
http://dx.doi.org/10.7774/cevr.2021.10.3.259
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