Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers

In this work, it is shown that surface-enhanced Raman scattering (SERS) measurements can be performed using liquid platforms to perform bioanalysis at sub-pM concentrations. Using magnetic enrichment with gold-coated magnetic nanoparticles, the high sensitivity was verified with nucleic acid and pro...

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Detalles Bibliográficos
Autores principales: Ji, Zhang, Zhang, Chuan, Ye, Yang, Ji, Jiali, Dong, Hongguang, Forsberg, Erik, Cheng, Xiaoyu, He, Sailing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511622/
https://www.ncbi.nlm.nih.gov/pubmed/34660557
http://dx.doi.org/10.3389/fbioe.2021.735711
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author Ji, Zhang
Zhang, Chuan
Ye, Yang
Ji, Jiali
Dong, Hongguang
Forsberg, Erik
Cheng, Xiaoyu
He, Sailing
author_facet Ji, Zhang
Zhang, Chuan
Ye, Yang
Ji, Jiali
Dong, Hongguang
Forsberg, Erik
Cheng, Xiaoyu
He, Sailing
author_sort Ji, Zhang
collection PubMed
description In this work, it is shown that surface-enhanced Raman scattering (SERS) measurements can be performed using liquid platforms to perform bioanalysis at sub-pM concentrations. Using magnetic enrichment with gold-coated magnetic nanoparticles, the high sensitivity was verified with nucleic acid and protein targets. The former was performed with a DNA fragment associated with the bacteria Staphylococcus aureus, and the latter using IgG antibody, a biomarker for COVID-19 screening. It is anticipated that this work will inspire studies on ultrasensitive SERS analyzers suitable for large-scale applications, which is particularly important for in vitro diagnostics and environmental studies.
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spelling pubmed-85116222021-10-14 Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers Ji, Zhang Zhang, Chuan Ye, Yang Ji, Jiali Dong, Hongguang Forsberg, Erik Cheng, Xiaoyu He, Sailing Front Bioeng Biotechnol Bioengineering and Biotechnology In this work, it is shown that surface-enhanced Raman scattering (SERS) measurements can be performed using liquid platforms to perform bioanalysis at sub-pM concentrations. Using magnetic enrichment with gold-coated magnetic nanoparticles, the high sensitivity was verified with nucleic acid and protein targets. The former was performed with a DNA fragment associated with the bacteria Staphylococcus aureus, and the latter using IgG antibody, a biomarker for COVID-19 screening. It is anticipated that this work will inspire studies on ultrasensitive SERS analyzers suitable for large-scale applications, which is particularly important for in vitro diagnostics and environmental studies. Frontiers Media S.A. 2021-09-29 /pmc/articles/PMC8511622/ /pubmed/34660557 http://dx.doi.org/10.3389/fbioe.2021.735711 Text en Copyright © 2021 Ji, Zhang, Ye, Ji, Dong, Forsberg, Cheng and He. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Ji, Zhang
Zhang, Chuan
Ye, Yang
Ji, Jiali
Dong, Hongguang
Forsberg, Erik
Cheng, Xiaoyu
He, Sailing
Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers
title Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers
title_full Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers
title_fullStr Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers
title_full_unstemmed Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers
title_short Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers
title_sort magnetically enhanced liquid sers for ultrasensitive analysis of bacterial and sars-cov-2 biomarkers
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511622/
https://www.ncbi.nlm.nih.gov/pubmed/34660557
http://dx.doi.org/10.3389/fbioe.2021.735711
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